Molecularly Imprinted Chitosan Modified Quartz Tuning Fork Sensors for Real Time Biosensing in Liquid Environment

Gizem Kaleli Can

doi:10.29130/dubited.1351846

EN TR

Molecularly Imprinted Chitosan Modified Quartz Tuning Fork Sensors for Real Time Biosensing in Liquid Environment

Abstract

Several new sensing technologies have emerged to meet the escalating demand for accurate and rapid diagnosis. We present an overview of the development of highly sensitive and selective Quartz Tuning Fork (QTF)-based sensors in a liquid environment, which will be critically important for contemporary diagnostic methods reliant on sensing technologies. The purpose of this study is to modify QTF prongs using molecularly imprinted chitosan, in combination with the operation of a quartz tuning fork as a piezoelectric crystal for biomedical applications. Through real-time data acquisition, we evaluate QTF resonance frequency shifts in dry and liquid environments using a model protein, BSA. As a result, the QTF-based sensor fails to detect BSA in dry conditions. It is however possible to measure frequency shifts ranging from 5 to 25 µg /mL within a liquid matrix. There is a rapid equilibration response time of 2 to 10 minutes depending on the concentration of BSA in the sensor. With the developed QTF-based sensor, a sensitivity of 1.1069 Hz/ µg has been achieved within the liquid matrix. As a result of the excellent properties of molecularly imprinted chitosan, it has been possible to develop a QTF-based biosensor capable of acquiring real-time data even when it is in liquid solutions.

Keywords

Quartz tuning fork, Mass sensitive sensor, Molecularly imprinted polymer, chitosan, bovine serum albumin

Sıvı Ortamda Gerçek Zamanlı Biyoalgılama için Moleküler Baskılı Kitosan Modifiye Kuvars Ayar Çatalı Sensör Geliştirilmesi

Abstract

Yüksek hassasiyet ve seçicilikle çalışan, doğru ve hızlı teşhis talebini karşılamak üzere bir dizi yeni duyusal teknoloji ortaya çıkmıştır. Bu çalışma kapsamında, sensör teknolojilerine dayalı çağdaş teşhis yöntemleri için kritik önem taşıyan sıvı ortamda çalışan Kuvars Ayar Çatal (QTF) tabanlı sensörler tasarımı üzerine detaylı bilgi sunmaktadır. Bu çalışma, biyomedikal uygulamalar için bir çevirici olarak kuvars ayar çatalın piezoelektrik kristal olarak kullanılması ile, QTF çatallarını moleküler baskılı kitosan ile modifiye etmeyi amaçlamaktadır Gerçek zamanlı veri toplama ile, model bir protein olan BSA kullanarak QTF rezonans frekansındaki değişimleri hem kuru hem de sıvı ortamlarda takip edilmiştir. Elde edilen sonuçlara göre, QTF tabanlı sensör, kuru koşullarda BSA tespiti yapamamaktadır. Ancak sıvı matris içinde 5 ila 25 µg/mL aralığında frekans değişimleri ölçülebildiği gösterilmiştir. Sensördeki BSA konsantrasyonuna bağlı olarak, tepki süresi BSA konsantrasyonuna bağlı olarak 2 ila 10 dakika arasında değişmektedir. Geliştirilen QTF tabanlı sensörle, sıvı matris içinde 1.1069 Hz/ µg hassasiyette ölçüm yapabilmektedir. Moleküler baskılı kitosanın üstün özellikleri sayesinde, sıvı çözeltilerde bile gerçek zamanlı veri toplama yeteneğine sahip bir QTF tabanlı biyosensör geliştirilmiştir.

Keywords

Kuvars ayar çatalı, Kütle hassas sensör, Moleküler baskılı polimer, Kitosan, Sığır serum albumin

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Details

Primary Language

English

Subjects

Sensor Technology

Journal Section

Research Article

Authors

Gizem Kaleli Can ^*
0000-0002-4411-622X
Türkiye

Publication Date

January 26, 2024

Submission Date

August 29, 2023

Acceptance Date

October 9, 2023

Published in Issue

Year 2024 Volume: 12 Number: 1

DOI

https://doi.org/10.29130/dubited.1351846

IZ

https://izlik.org/JA92TJ94HW

APA

Kaleli Can, G. (2024). Molecularly Imprinted Chitosan Modified Quartz Tuning Fork Sensors for Real Time Biosensing in Liquid Environment. Duzce University Journal of Science and Technology, 12(1), 337-347. https://doi.org/10.29130/dubited.1351846

AMA

1.Kaleli Can G. Molecularly Imprinted Chitosan Modified Quartz Tuning Fork Sensors for Real Time Biosensing in Liquid Environment. DUBİTED. 2024;12(1):337-347. doi:10.29130/dubited.1351846

Chicago

Kaleli Can, Gizem. 2024. “Molecularly Imprinted Chitosan Modified Quartz Tuning Fork Sensors for Real Time Biosensing in Liquid Environment”. Duzce University Journal of Science and Technology 12 (1): 337-47. https://doi.org/10.29130/dubited.1351846.

EndNote

Kaleli Can G (January 1, 2024) Molecularly Imprinted Chitosan Modified Quartz Tuning Fork Sensors for Real Time Biosensing in Liquid Environment. Duzce University Journal of Science and Technology 12 1 337–347.

IEEE

[1]G. Kaleli Can, “Molecularly Imprinted Chitosan Modified Quartz Tuning Fork Sensors for Real Time Biosensing in Liquid Environment”, DUBİTED, vol. 12, no. 1, pp. 337–347, Jan. 2024, doi: 10.29130/dubited.1351846.

ISNAD

Kaleli Can, Gizem. “Molecularly Imprinted Chitosan Modified Quartz Tuning Fork Sensors for Real Time Biosensing in Liquid Environment”. Duzce University Journal of Science and Technology 12/1 (January 1, 2024): 337-347. https://doi.org/10.29130/dubited.1351846.

JAMA

1.Kaleli Can G. Molecularly Imprinted Chitosan Modified Quartz Tuning Fork Sensors for Real Time Biosensing in Liquid Environment. DUBİTED. 2024;12:337–347.

MLA

Kaleli Can, Gizem. “Molecularly Imprinted Chitosan Modified Quartz Tuning Fork Sensors for Real Time Biosensing in Liquid Environment”. Duzce University Journal of Science and Technology, vol. 12, no. 1, Jan. 2024, pp. 337-4, doi:10.29130/dubited.1351846.

Vancouver

1.Gizem Kaleli Can. Molecularly Imprinted Chitosan Modified Quartz Tuning Fork Sensors for Real Time Biosensing in Liquid Environment. DUBİTED. 2024 Jan. 1;12(1):337-4. doi:10.29130/dubited.1351846

Molecularly Imprinted Chitosan Modified Quartz Tuning Fork Sensors for Real Time Biosensing in Liquid Environment

Abstract

Keywords

Sıvı Ortamda Gerçek Zamanlı Biyoalgılama için Moleküler Baskılı Kitosan Modifiye Kuvars Ayar Çatalı Sensör Geliştirilmesi

Abstract

Keywords

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

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