Spektrofotometrik Yöntemlerle Tabletlerde Metimazol Tayini

Yıl 2019, , 904 - 910, 31.01.2019

Saadet Dermiş , Sinan Beyaztaş Zehra Ceren Ertekin , Erdal Dinç

https://doi.org/10.29130/dubited.449055

Öz

Bu çalışma doğrudan absorbans ölçüm ve türev spektrofotometrik yöntemlerini kullanarak tabletlerdeki
methimazolün spektrofotometrik miktar tayinini açıklamaktadır. Yapılan ön çalışmalarda sıfırıncı derece ve
birinci türev spektrofotometri yöntemlerinde doğrusal çalışma aralığı 2,0-24,0 µg/mL olarak bulunmuştur.
Sıfırıncı derece spektrofometri yönteminde 260,0 nm, birinci derece türev spektrofotometri yönteminde ise 269,0
nm dalga boylarında ölçümler yapılarak kalibrasyon eğrileri elde edilmiştir. Geri kazanım ve bağıl standart
sapma değerleri; doğrudan absorbans ölçüm yöntemi için % 103,3 ve % 2,18 ve birinci derece türev
spektrofotometri için % 98,0 ve % 0,64 olarak bulunmuştur. Daha sonra önerilen teknikler tablet preparatlarının
analizi için uygulanmıştır. Bu çalışmada metodların uygulanabilirliği, methimazol içeren test örneklerinin
validasyon çalışmalarıyla doğrulanmıştır. Analiz sonuçları, ilacın miktar tayini için ilgili metodların
uygulanabilir olduğunu göstermektedir. 

Anahtar Kelimeler

Methimazol, UV-GB Spektrofotometri, Tablet

Determination of Methimazole in Tablets Using Spectrophotometric Methods

Öz

This study describes the spectrophotometric quantification of methimazole in tablets using zero-order absorption
spectrophotometry and first derivative spectrophotometry methods. In preliminary experiments, linearity range
was found between 2.0-24.0 µg/mL in zero-order spectrophotometry and first-order derivative
spectrophotometry. Calibration curves were obtained by measurements of the zero-order absorbances and their
first derivative values at 260.0 and 269.0 nm for the zero-order spectrophotometry and first derivative
spectrophotometry, respectively. The recovery and relative standard deviations were calculated as 103.3 % and
2.18 % for UV absorption spectrophotometry and 98.0% and 0.64% for first derivative spectrophotometry,
respectively. The proposed techniques were used for the analysis of tablets preparations. The methods proposed
in this study were verified by analyzing validation test samples containing methimazole. Determination results
showed that the applied methods were found to suitable for the quantitation of the related drug. 

Anahtar Kelimeler

Methimazole, UV-VIS Spectrophotometry, Tablet

Kaynakça

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