Tryptamine Biosensor Based on Amino-Functionalized Multiwalled Carbon Nanotubes, Tin Oxide Nanoparticles and Diamine Oxidase

Abstract

In this study, amperometric tryptamine biosensor based on amino functionalized multiwalled carbon nanotubes (NH₂-MWCNT) and tin oxide nanoparticles (SnO₂) modified screen-printed carbon electrode (SPCE) was developed. Diamine oxidase (DAO) enzyme was covalently immobilized onto NH₂-MWCNT-SnO₂/SPCE surface via (1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide) (EDC) ve N-hydroxysuccinimide (NHS) chemistry. The resulting electrode surface was finally covered with Nafion in order to prevent enzyme leakage from the surface and minimize the effect of interferences. The surface morphology, electrochemical bahaviour and analytical performance of the biosensor was investigated by scanning electron microscopy (SEM), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and chronoamperometry methods. Linear working range, limit of detection and sensitivity of the developed biosensor was found to be 2.0×10^-6 ‒ 2.5×10^-3 M, 6.0×10^-7 M ve 6.52 µA mM^-1, respectively. Biosensor also showed high repeatability and reproducibility.

Keywords

• Biogenic amine,Tryptamine,Biosensor,Carbon nanotube

Amin Fonksiyonlu Karbon Nanotüp, Kalay Oksit Nanopartikül ve Diamin Oksidaz Temelli Triptamin Biyosensörü

Abstract

Bu çalışmada amino fonksiyonlu çok duvarlı karbon nanotüp (NH₂-MWCNT) ve kalay oksit nanopartikül (SnO₂) ile modifiye edilmiş perde baskılı karbon elektrotlara (SPCE) dayanan amperometrik triptamin biyosensörü geliştirildi. Diamin oksidaz (DAO) enzimi NH₂-MWCNT-SnO₂/SPCE yüzeyine N-etil-N′-(3-dimetilaminopropil) karbodiimit (EDC) ve N-hidroksi süksinimit (NHS) kullanılarak kovalent bağlama yöntemi ile immobilize edildi. Hazırlanan elektrot yüzeyi, enzimlerin yüzeyden uzaklaşmasını engellemek ve girişim etkilerini azaltmak amacıyla son olarak Nafyon ile kaplandı. Biyosensörün yüzey morfolojisi, elektrokimyasal özellikleri ve analitik performansı taramalı elektron mikroskobu (SEM), dönüşümlü voltammetri (CV), elektrokimyasal empedans spektroskopi (EIS) ve kronoamperometri yöntemleri kullanılarak incelendi. Geliştirilen biyosensör ile triptamin için elde edilen doğrusal çalışma aralığı, gözlenebilme sınırı ve duyarlık sırası ile 2,0×10^-6 ‒ 2,5×10^-3 M, 6,0×10^-7 M ve 6,52 µA mM^-1 olarak bulundu. Hazırlanan biyosensörün tekrar kullanılabilirlik ve tekrar üretilebilirliğinin oldukça iyi olduğu belirlendi.

Keywords

• Biyojenik amin,Triptamin,Biyosensör,Karbon nanotüp,Kalay oksit nanopartikül

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