Labellum Micromorphology in Some Orchis Taxa

Abstract

In the study, the micromorphology of labellum in samples belonging to 9 different Orchis taxa [Neotinea tridentata (Scop.) R.M.Bateman, Pridgeon & M.W.Chase (Syn. O. tridentata Scop.), O. punctulata Stev. ex Lindl., O. purpurea Huds., O. simia Lam., Anacamptis morio (L.) R. M. Bateman, Pridgeon & M. W. Chase (Syn. O. morio L. subsp. morio), Orchis picta Raf. (Syn: O. morio L. subsp picta (Lois.) K.Richt., Anacamptis papilionacea (L.) R. M. Bateman, Pridgeon & M. W. Chase (Syn: O. papilionacea L. var. papilionacea), Anacamptis laxiflora (Lam.) R.M.Bateman, Pridgeon & M.W.Chase (Syn: O. laxiflora Lam.), Himantoglossum robertianum (Loisel.) P. Delforge (Syn: O. fragrans Pollini)] were examined by Scanning Electron Microscopy (SEM) method and the results are presented as a diagnostic key. While the samples were collected from the field, they were taken to 70% alcohol, some of them were dried according to the herbarium rules and identified. The labellum samples extracted from alcohol were dissected under the stereomicroscope with a scalpel and needles and placed directly on the iron apparatus of the SEM device with double-sided adhesive tapes. These samples, whose alcohol was completely evaporated by waiting for one night, were examined directly with Scanning Electron Microscope (SEM) and labellum microphotographs were taken. According to our findings; O. fragrans, O. papilionacea, O. purpurea are quite different from all other examined taxa, others generally have conical papillae formations in different derivatives peculiar to them, In some species (O. punctulata, O. purpurea, O. simia, O. laxiflora, O. fragrans) apex and / or spur regions were found to have remarkable differences. The features of these papillae and the regional overlap on the labellum surface were distinctive for some taxa. Especially, whether the epidermal cells in the apex area contain papillae, the cells' flat or different sculptural features, the regular or irregularity of the cells, the characteristics of their lateral walls have created important differences among taxa.

Keywords

• Orchidaceae
• Orchis
• Flower
• Labellum
• Morphology
• Petal

Bazı Orchis Taksonlarında Labellum Mikromorfolojisi

Abstract

Çalışmada 9 farklı Orchis taksonununa [Neotinea tridentata (Scop.) R.M.Bateman, Pridgeon & M.W.Chase (Syn. O. tridentata Scop.), O. punctulata Stev. ex Lindl., O. purpurea Huds., O. simia Lam., Anacamptis morio (L.) R. M. Bateman, Pridgeon & M. W. Chase (Syn. O. morio L. subsp. morio), Orchis picta Raf. (Syn: O. morio L. subsp picta (Lois.) K.Richt., Anacamptis papilionacea (L.) R. M. Bateman, Pridgeon & M. W. Chase (Syn: O. papilionacea L. var. papilionacea), Anacamptis laxiflora (Lam.) R. M. Bateman, Pridgeon & M. W. Chase (Syn: O. laxiflora Lam.), Himantoglossum robertianum (Loisel.) P. Delforge (Syn: O. fragrans Pollini)] ait örneklerde labellum mikromorfolojisi Taramalı Elektron mikroskobu (SEM) yöntemi ile incelenmiş ve sonuçlar teşhis anahtarı halinde sunulmuştur. Örnekler, araziden toplandığı sırada %70 alkole alınmış, bir kısmı da herbaryum kurallarına göre kurutularak teşhisleri yapılmıştır. Alkolden çıkarılan labellum örnekleri, bisturi ve iğnelerle stereomikroskop altında kesip ayrılarak doğrudan, üzeri çift taraflı yapışkan bantlar bulunan ve SEM cihazına ait çelik aparatlara yerleştirilmiştir. Bir gece kadar bekletilerek alkolü tamamıyla uçurulan bu örnekler, doğrudan Taramalı Elektron Mikroskobu (SEM) ile incelenmiş ve labellum mikrofotoğrafları çekilmiştir. Bulgularımıza göre; O. fragrans, O. papilionacea, O. purpurea taksonlarının diğer tüm incelenen taksonlardan oldukça farklı olduğu, diğerlerinde genelde kendilerine özgü değişik türevlerde konikal papilla oluşumlarının bulunduğu, bazı türlerde (O. punctulata, O. purpurea, O. simia, O. laxiflora, O. fragrans) uç ve/veya mahmuz bölgelerinin dikkat çekici farklılıklara sahip olduğu görülmüştür. Söz konusu papillaların özellikleri ve labellum yüzeyindeki bölgesel örtüşü, bazı taksonlar için ayırt edici olmuştur. Bilhassa uç bölgesindeki epidermal hücrelerin papilla içerip içermemesi, hücrelerin düz veya farklı yüzey morfoloji (skülptür) özellikleri, hücrelerin düzenli ya da düzensiz olması, yanal çeperlerinin özellikleri taksonlar arasında önemli farklılıkları oluşturmuştur.

Keywords

• Orchidaceae
• Orchis
• Çiçek
• labellum
• morfoloji
• petal

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