Türkiye'de Yetişen Üç Ahlat Türünün Arbutin İçeriğinin Değerlendirilmesi

Yıl 2020, Cilt: 8 Sayı: 1, 81 - 93, 31.01.2020

Rukiye Kayhan Safiye Elif Korcan İbrahim Bulduk Mustafa Kargıoğlu Emrah Şelli

https://doi.org/10.29130/dubited.556150

Cited By: 2

Öz

Armut (Pyrus spp.) Dünya çapında en önemli meyvelerden biridir. Pyrus cinsi, Rosaceae ailesinin Pomoideae alt ailesine aittir. Pyrus türlerinin farklı organları farklı oranlarda arbutin içerir. Arbutin birçok tıbbi bitkide bulunan ve doğal olarak oluşan glikozittir. Arbutin, Lamiaceae, Ericaceae, Saxifragaceae ve Rosaceae gibi farklı familyalardan gelen farklı bitki türlerinde bulunur. Tirosinazı önleme kabiliyetinden dolayı beyazlatıcı bir madde olarak kullanılır. Bu çalışmanın amacı, Afyonkarahisar ilinde yetişen Pyrus Species (PEL (PEL), PAM (PAM) ve PAN (PAN)) 'nin arbutin içeriğini tespit etmektir. Örnekler oda sıcaklığında onbeş gün boyunca kurutuldu. Tüm yabani pyrus türlerinin arbutin içerikleri, ters faz HPLC ile belirlendi. UV dedektörü ile donatılmış bir Agilent 1260 HPLC sistemi üzerinde miktar tayini yapılmıştır. Bir C18 (5 um, 250 mm x 4.6 mm) analitik kolon kullanıldı. Mobil faz% 93 deiyonize su ve% 7 metanoldü. İzokratik elüsyon, 1 mL / dak akış hızında kullanıldı. Kolon sıcaklığı 25 ° C'de tutuldu ve tespit dalga boyu arbutin için 280 nm'de ayarlandı. Analitik yöntem, ICH Q7A Kılavuzuna göre doğrulandı. Çalışmamızın sonuçları üç pyrus türünün de arbutin içerdiğini göstermiştir. Yaprakların arbutin içeriği, üç yabani armutun meyvelerinden daha yüksektir. Her üç türün de yapraklarında yüksek oranda arbutin içeriği bulunmuştur. PAN, diğer türlerden daha yüksek oranda arbutin oranına sahiptir (Yaprak: 21,23 mg / g. Dal: 8,92 mg / g. Meyve: 0,81 mg / g).

Anahtar Kelimeler

Yabani ahlat türleri, Arbutin, Hplc

Evaluation of Arbutin Content of Three Wild Pear Species Growing in Turkey

Öz

Pear (Pyrus spp.) is one kind of the most important worldwide fruit. The genus Pyrus belongs to the subfamily Pomoideae of family Rosaceae. Different organs of the Pyrus species contain arbutin in different proportions. Arbutin is a naturally occurring glycoside found in many medicinal plants. Arbutin is found in different plant species from different families such as Lamiaceae, Ericaceae, Saxifragaceae and Rosaceae. Due to its ability to inhibit tyrosinase, it is used as a whitening agent. The aim of this study was to determine arbutin content of Pyrus Species (PEL(PEL), PAM(PAM) and PAN(PAN)) growing in Afyonkarahisar province of Turkey. Samples were dried at room temperature for fifteen days. Arbutin contents of all wild pyrus species were determined by reversed phase HPLC. Quantification was performed on an Agilent 1260 HPLC system equipped with a UV detector. A C18 (5 μm, 250 mm x 4.6 mm) analytical column was used. Mobile phase was 93% deionized water and 7% methanol. İsocratic elution was used at a flow rate of 1 mL / min. The column temperature was maintained at 25 0C and the detection wavelength was set at 280 nm for arbutin. The analytical method was validated according to ICH Q7A Guideline. The results of our study showed that all three pyrus species contain arbutin. The arbutin content in the leaves is higher than the fruits of all three wild pears. A high proportion of arbutin content was found in the leaves of all three types. PAN has a higher proportion of arbutin than the other species (Leaf: 21,23 mg / g. Brunch: 8,92 mg / g. Fruit: 0,81 mg / g).

Anahtar Kelimeler

Pyrus spp, Arbutin, Hplc

Kaynakça

• [1] P. H. Davis (eds), “Flora of Turkey and the East Aegean Islands,” Edinburgh University, Edinburgh, vol. 4, 1972.
• [2] H. I. Guk, K. H. Young, W. K. Sik, L. S. Hoon, L. Junsoo and J. H. Sang, “ Isolation and Identification of the Antioxidant DDMP from Heated Pear (Pyrus pyrifolia Nakai),” Prev. Nutr. Food Sci., 18(1):76-79, 2013.
• [3] P. Dwıvedı and P. Prakash, “Evaluation of Antioxidant Activity of Pyrus Pyrifolia Fruit Peel Extracts Using Different Extraction Methods,” Chemical Science Transactions, 3(4):1511-1515, 2014.
• [4] J. Y. Cho, C. M. Kim, H. J. Lee, S. H. Lee, J. A. Cho, W. S. Kim, et al., “ Caffeoyl triterpenes from pear (Pyrus pyrifolia Nakai) fruit peels and their antioxidative activities against oxidation of rat blood plasma,” Journal of Agricultural and Food Chemistry, 61(19):4563–4569, 2013.
• [5] K. Ioku, J. Terao, N. Nakatani, “Antioxidative activity of arbutin in a solution and liposomal suspension,” Biosci Biotechnol Biochem, 56:1658–1659, 1992.
• [6] S. H. Bang, S.J. Han, D. H. Kim, “Hydrolysis of arbutin to hydroquinone by human skin bacteria and its effect on antioxidant activity,” J Cosmet Dermatol, 7:189–193, 2008.
• [7] K. Jurica, I. Gobin, D. Kremer, D. V. Cepo, R. J. Grubesic, I. B. Karaconji, I. Kosalec, “Arbutin and its metabolite hydroquinone as the main factors in the antimicrobial effect of strawberry tree (Arbutus unedo L.) leaves,” J Herb Med, 8:17–23, 2017. [8] D. Jiri, "Pyrus spinosa und ihre Hybriden in Südwestbulgarien,” Folia Geobotanica & Phytotaxonomica (in German), 15 (1): 59–73, 1980.
• [9] İ. Bulduk, M. D. Sahin, S. Sanli, “Arbutin Analysis in Leaves, Fruit and Branches of Pyrus Anatolica, Method Optimization,” Eurasian Journal of Analytical Chemistry, 11(5):233–244, 2016.
• [10] N. J. Rivas, J. R. Whitaker, “Purification and Some Properties of Two Polyphenol Oxidases from Bartlett Pears,” Plant Physiology, 52:501–507, 1973.
• [11] A. Chisvert, J. Sisternes, A. Balaguer, A. Salvado, “A gas chromatography-mass spectrometric method to determine skin-whitening agents in cosmetic products,” j.talanta, Talanta, 81:530-6, 2010.
• [12] European Medicines Agency Assessment report on Arctostaphylos uva-ursi (L.) Spreng., folium, displayed 14 September 2015, Available at http://www.ema.europa.eu/ docs/en GB/document_library/Herbal_-HMPCsessment_report/2011/07/WC500108750.pdf.
• [13] M. Blaut, A. Braune, S. Wunderlich, P. Sauer, H. Schneider, H. Glatt, “Mutagenicity of arbutin in mammalian cells after activation by human intestinal bacter,” Food Chem Toxicol, 44:1940-7, 2006.
• [14] D.C Hildebrand, CC. Jr. Powell, S. M. Schroth, “Fire blight resistance in Pyrus: localization of arbutin and betaglucosidase,” Phytopathology, 59:1534-9, 1969.
• [15] G. Schindler, U. Patzak, B. Brinkhaus, A. von Niecieck, J. Wittig, N. Krähmer, I. Glöckl, M. Veit, “Urinary excretion and metabolism of arbutin after oral administration of Arctostaphylos uvae ursi extract as film-coated tablets and aqueous solution in healthy humans,” J Clin Pharmacol, 42:920-7, 2002.
• [16] S. G. Arriba, B. Naser, K-U. Nolte, “Risk assessment of free hydroquinone derived from Arctostaphylos uva-ursi folium herbal preparations,” Int J Toxicol, 32:442-53, 2014.
• [17] B. Lukas, C. Schmiderer, U. Mitteregger, J. Novak, “Arbutin in marjoram and oregano,” Food Chem, 121:185–90, 2010.
• [18] I. Parejo, F. Viladomat, J. Bastida, C. Codina, “ A single extraction step in the quantitative analysis of arbutin in bearberry (Arctostaphylos uva-ursi) leaves by high-performance liquid chromatography,” Phytochem Anal, 12:336-9, 2001.
• [19] R. D. PavloviC, B. LakuSiC, Z. DoSlov-Kokorus, N. Kovacevic, “ Arbutin content and antioxidant activity of some Ericaceae species,” Pharmazie, 64:656-9, 2009.
• [20] I. Fecka, S. Turek, “Determination of polyphenolic compounds in commercial herbal drugs and spices from Lamiaceae: thyme, wild thyme and sweet marjoram by chromatographic techniques,” Food Chem, 108:1039-53, 2008.
• [21] I. Rychlinska, S. Nowak, “Quantitative determination of arbutin and hydroquinone in different plant materials by HPLC,” Not Bot Horti Agrobo, 40:109-13, 2012.
• [22] C. Pop, L. Vlase, M. Tamas, “Natural resources containing arbutin. Determination of arbutin in the leaves of Bergenia crassifolia (L.) Fritsch. acclimated in Romania,” Not Bot Horti Agrobo, 37:129-32, 2009.
• [23] A. Lamien-Meda, B. Lukas, C. Schmiderer, C. Franz, J. Novak, “Validation of a quantitative assay of arbutin using gas chromatography in Origanum majorana and Arctostaphylos uva-ursi extracts,” Phytochem Anal, 20:416–20, 2009.
• [24] A. Fiorentino, S. Castaldi, B. D’Abrosca, A. Natale, A. Carfora, A. Messere, P. Monaco, “Polyphenols from the hydroalcoholic extract of Arbutus unedo living in a monospecific Mediterranean woodland,” Biochem Syst Ecol, 35:809- 11, 2007.
• [25] A. Pyka, K. Bober, A. Stolarczyk, “Densitometric determination of arbutin in cowberry leaves (Vaccinium vitis idaea),” Acta Pol Pharm, 64:395-400, 2007.
• [26] ICH Q7 Guideline: Good Manufacturing Practice Guide For Active Pharmaceutical Ingredients. Current Version Dated 10 June 2015.
• [27] J. Salta, A. Martins, R. G. Santos, N. R. Neng, M. F. Jose, “Nogueira, Jorge Justino, Ame´lia P. Rauter. Phenolic composition and antioxidant activity of Rocha pear and other pear cultivars – A comparative study,” Journal of Functional Foods, pp. 153-157, 2010.
• [28] S-H Yim, S-H Nam, “Physiochemical, nutritional and functional characterization of 10 different pear cultivars (Pyrus spp.),” Journal of Applied Botany and Food Quality, 89:73-81, 2016.
• [29] I. Rychlınska, S. Nowak, “Quantitative Determination of Arbutin and Hydroquinone in Different Plant Materials by HPLC,” Not Bot Horti Agrobo, 40(2): 109-113, 2012.
• [30] X. Dong, Y. Zheng, Y. Cao, L. Tian, Y. Zhang, D. Qi1, H. Huo, D. Wang, “Evaluation of Phenolic Composition and Content of Pear Varieties in Leaves from China,” Erwerbs-Obstbau, 60:331–340, 2018.
• [31] K. Jurica, I. Brcic Karaconji, A. Mikolic, D. Milojkovic Opsenica, V. Benkovic, N. Kopjar, “In vitro safety assessment of the strawberry tree (Arbutus unedo L.) water leaf extract and arbutin in human peripheral blood lymphocytes,” Cytotechnology, 70:1261–1278, 2018.
• [32] T. Khana, H. G. Kim, Y. P. Hwang, M. J. Kong, M. J. Kang, H. K. Yeo, D. H. Kim, T. C. Jeong, H. G. Jeong, “Role of metabolism by the human intestinal microflora in arbutin-induced cytotoxicity in HepG2 cell cultures,” Biochemical and Biophysical Research Communications, vol. 413, pp. 318-324, 2011.
• [33] A. Badiab, F. Nabbie, M. C. Tettamanzi, N. Patel, N. Jain and B. Peethambaran, “Research Article Specific Cytotoxicity of a Novel Arbutin Derivative from Myrothamnus flabellifolius Against Human Leukemia Cells,” Research Journal of Medicinal Plants, 10:396-402, 2016.
• [34] T. Kundakovic, A. Ciric, T. Stanojkovic, M. Sokovic and N. Kovacevic, “Cytotoxicity and antimicrobial activity of Pyrus pyraster Burgsd. and Pyrus spinosa Forssk. (Rosaceae),” African Journal of Microbiology Research, vol. 8(6), pp. 511-518, 5 February 2014.
• [35] I. Berdowska, B. Zielinski, I. Fecka, J. Kulbacka, J. Saczko, A. Gamian, “Cytotoxic impact of phenolics from Lamiaceae species on human breast cancer cells,” Elsevier Food Chemistry, vol. 141, pp. 1313-1321, 2013.
• [36] J-H. Hong, H-J. Chen, S-J. Xiang, S-W. Cao, B-C. An, S-F. Ruan, B. Zhang, L-D. Weng, H-X Zhu, Q. Liu, “Capsaicin reverses the inhibitory effect of licochalcone A/β-Arbutin on tyrosinase expression in b16 mouse melanoma cells,” vol. 14, pp. 110-115, 2018.