Yıl 2020, Cilt 8 , Sayı 1, Sayfalar 693 - 701 2020-01-31

Endemik Alcea calvertii (Boiss) Boiss. Çiçeklerinin In vitro Antiradikal, Antimikrobiyal ve Antiproliferatif Aktiviteleri ve Fitokimyasal Kompozisyonu
In vitro Antiradical, Antimicrobial and Antiproliferative Activities and Phytochemical Compositions of Endemic Alcea calvertii (Boiss) Boiss. Flowers

Serhat KESER [1] , Fatma KESER [2] , Suat TEKIN [3] , Ömer KAYGILI [4] , İsmail TURKOGLU [5] , Ersin DEMIR [6] , Mustafa KARATEPE [7] , Sevda KIRBAG [8] , Ökkes YILMAZ [9] , Suleyman SANDAL [10] , Semra TURKOGLU [11]


Alcea calvertii (Boiss) Boiss. Malvaceae familyasında yer alan çok yıllık otsu endemik bir bitkidir. Alcea genusuna ait bitkiler bahçelerde süs bitkileri olarak yetiştirilirler. Sunulan çalışmada, Alcea calvertii çiçeklerinin su, etanol, metanol ve aseton ekstraktlarının antiproliferatif, antimikrobiyal, antiradikal aktiviteleri ve fitokimyasal kompozisyonları incelendi. A. calvertii çiçeklerinin su, etanol, metanol ve aseton ekstraktlarının standart antioksidan trolokstan daha düşük oranda DPPH, ABTS ve OH radikali yok ettiği saptandı. A. calvertii çiçeklerinin vitamin, sterol, flavonoit ve fenolik asitler içerdiği ve yüksek miktarda vanillik asit, gallik asit, kateşin, δ-tokoferol, ergosterol ve vitamin D içerdiği belirlendi. A. calvertii su ekstraktının MCF-7 ve HCT-116 kanser hücrelerine karşı diğer ektstraktlardan daha iyi antiproliferatif aktivite gösterdiği gözlendi. LNCaP hücre serilerine karşı ise A. calvertii metanol ekstraktı daha yüksek antiproliferatif aktivite gösterdi.

Alcea calvertii (Boiss) Boiss. is belonged to Malvaceae family, and it is a perennial herbaceous endemic plant. Alcea genus plants are grown as ornamental plants in the gardens. In the present study, the antiproliferative, antimicrobial, antiradical activities and phytochemical compositions of ethanol, water, methanol and acetone extracts of A. calvertii flowers were examined. A. calvertii flowers water, ethanol, methanol and acetone extracts are lower scavenged DPPH, ABTS and OH radicals than standard antioxidant trolox. A. calvertii flowers contain vitamins, sterols, flavonoids and phenolic acids, dominated by vanillic acid, gallic acid, catechin, δ-tocopherol, ergosterol and vitamin D. A. calvertii flowers water extract showed better antiproliferative activities than other extracts against to MCF-7 and HCT-116 cell lines. A. calvertii flowers methanol extract showed higher antiproliferative effect against to LNCaP cell lines.

  • [1] N.M. Ammar, E.A. El-Kashoury, L.T. Abou El-Kassem, and R.E. Abd El-Hakeem, “Evaluation of the phenolic content and antioxidant potential of Althaea rosea cultivated in Egypt,” Journal of The Arab Society for Medical Research, vol. 8, pp. 48–52, 2013.
  • [2] P.H. Davis, Flora of Turkey and the East Aegean Islands (vol. 10), Edinburgh, United Kingdom: Edinburgh University Press, 1988.
  • [3] A.O. Sevinç, “Investigation of the Antimicrobial and Cytotoxic Effects of the Extracts Obtained from Alcea heldreichii (Boiss.) Boiss. (Malvaceae)” M.S. thesis (in Turkish), Institute of Science and Technology, Akdeniz University, Antalya, Turkey, 2014.
  • [4] H. Fersahoglu, “Bioactive Properties of Hollyhocks (Alcea rosea) Flowers in Different Colors,” M.S. thesis (in Turkish), Institute of Science and Technology, Yildiz Technical University, İstanbul, Turkey, 2016.
  • [5] M.E. Uzunhisarcikli, and M. Vural, “Yetersiz veri (DD) kategorisinde bulunan iki Alcea L. (Malvaceae) türünün yeni IUCN kategorileri ve taksonomisi,” Biological Diversity and Conservation, vol. 2, pp. 90–95, 2009
  • [6] M. Zakizadeh, S.F. Nabavi, S.M. Nabavi, and M.A. Ebrahimzadeh, “In vitro antioxidant activity of flower, seed and leaves of Alcea hyrcana Grossh,” European Review for Medical and Pharmacological Sciences, vol. 15, pp. 406–412, 2011.
  • [7] R. Re, N. Pellegrini, A. Proteggente, A. Pannala, M. Yang, and C. Rice-Evans, “Antioxidant activity applying an improved ABTS radical cation decolorization assay,” Free Radical Biology and Medicine, vol. 26, pp. 1231–1237, 1999.
  • [8] B. Halliwell, J.M.C. Gutteridge, and O. Aruoma, “The deoxyribose method: a simple test tube assay for determination of rate constants for reactions of hydroxyl radicals,” Analytical Biochemistry, vol. 165, pp. 215–219, 1987.
  • [9] W. Brand-Williams, M.E. Cuvelier, and C. Berset, “Use of a free radical method to evaluate antioxidant activity,” LWT-Food Science and Technology, vol. 28, pp. 25–30, 1995.
  • [10] K. Slinkard, and V.L. Singleton, “Total phenol analysis-automation and comparison with manual methods,” American Journal of Enology and Viticulture, vol. 28, pp. 49–55, 1977.
  • [11] D.O. Kim, O.K. Chun, Y.J. Kim, H.Y. Moon, and C.Y. Lee, “Quantification of polyphenolics and their antioxidant capacity in fresh plums,” Journal of Agricultural and Food Chemistry, vol. 51, pp. 6509–6515, 2003.
  • [12] O.U. Amaeze, G.A. Ayoola, M.O. Sofidiya, A.A. Adepoju-Bello, A.O. Adegoke, and H.A.B. Coker, “Evaluation of antioxidant activity of Tetracarpidium conophorum (Mull. Arg) Hutch & Dalziel leaves,” Oxidative Medicine and Cellular Longevity, Article ID 976701, 2011.
  • [13] Y.G. Zu, C.Y. Li, Y.J. Fu, and C.J. Zhao, “Simultaneous determination of catechin, rutin, quercetin, kaempferol and isorhamnetin in the extract of sea buckthorn (Hippophae rhamnoides L.) leaf by RP-HPLC with DAD,” Journal of Pharmaceutical and Biomedical Analysis, vol. 41, pp. 714–719, 2006.
  • [14] W.W. Christie, Gas chromatography and lipids, Glasgow, Scotland: The Oil Press, 1992.
  • [15] J. López-Cervantes, D.I. Sánchez-Machado, and N.J. Ríos-Vázquez, “High performance liquid chromatography method for the simultaneous quantification of retinol, α-tocopherol, and cholesterol in shrimp waste hydrolysate,” Journal of Chromatography A, vol. 1105, pp. 135–139, 2006.
  • [16] D.I. Sanchez-Machado, J. Lopez-Hernandez, and P. Paseiro-Losado, “High performance liquid chromatographic determination of alpha-tocopherol in macroalgae,” Journal of Chromatography A, vol. 976, pp. 277–284, 2002.
  • [17] C.M. Collins, and P.M. Lyne, Microbiological Methods, Buttermorths-Heinemann, London, England, 1989.
  • [18] T. Mosmann, “Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays,” Journal of Immunological Methods, vol. 65, pp. 55–63, 1983.
  • [19] F. Denizot, and R. Lang, “Rapid colorimetric assay for cell growth and survival modifications to the tetrazolium dye procedure giving improved sensitivity and reliability,” Journal of Immunological Methods, vol. 89, pp. 271–277, 1986.
  • [20] C. Anlas, O. Ustuner, F.U. Alkan, T. Bakirel, M.N. Aydogan, and S.B. Erel, “A comparative study on the antioxidant activities and phenolic contents of different extracts of Achillea nobilis subsp. sipylea and Alcea apterocarpa (Fenzl) Boiss, endemic plants in Turkey,” Fresenius Environmental Bulletin, vol. 26, pp. 1423–1430, 2017.
  • [21] A. Ertas, M. Boga, I. Gazioglu, Y. Yesil, N. Hasimi, C. Ozaslan, H. Yilmaz, and M. Kaplan, “Fatty acid, essential oil and phenolic compositions of Alcea pallida and Alcea apterocarpa with antioxidant, anticholiesterase and antimicrobial activities,” Chiang Mai Journal of Science, vol. 43, pp. 89–99, 2016.
  • [22] M. Benli, K. Gunay, U. Bingol, F. Guven, and N. Yigit, “Antimicrobial activity of some endemic plants species from Turkey,” African Journal of Biotechnology, vol. 6, pp. 1774–1778, 2007.
  • [23] S.M. Seyyednejad, H. Koochak, E. Darabpour, and H. Motamedi, “A survey on Hibiscus rosa-sinensis, Alcea rosea L. and Malva neglecta Wallr. as antibacterial agents,” Asian Pacific Journal of Tropical Medicine, vol. 3, pp. 351–355, 2010.
  • [24] T. Mert, T. Fafal, B. Kivcak, and H.T. Ozturk, “Antimicrobial and cytotoxic activities of extracts obtained from flowers of Alcea rosea L.,” Hacettepe University Journal of the Faculty of Pharmacy, vol. 30, pp. 17–24, 2010.
  • [25] N.A. Abdel-Salam, N.M. Ghazy, S.M. Sallam, M.M. Radwan, A.S. Wanas, M.A. El Sohly, M.A. El-Demellawy, N.M. Abdel-Rahman, S. Piacente, and M.L. Shenouda, “Flavonoids of Alcea rosea L. and their immune stimulant, antioxidant and cytotoxic activities on hepatocellular carcinoma HepG-2 cell line,” Natural Product Research, vol. 32, pp. 702–706, 2018.
  • [26] A.S. Yaglioglu, F. Eser, S. Tekin, and A. Onal, “Antiproliferative activities of several plant extracts from Turkey on rat brain tumor and human cervix carcinoma cell lines,” Frontiers in Life Science, vol. 9, pp. 69–74, 2016.
Birincil Dil en
Konular Mühendislik
Bölüm Makaleler
Yazarlar

Orcid: 0000-0002-9678-1053
Yazar: Serhat KESER (Sorumlu Yazar)
Kurum: FIRAT UNIVERSITY
Ülke: Turkey


Orcid: 0000-0001-6870-0546
Yazar: Fatma KESER
Kurum: FIRAT UNIVERSITY
Ülke: Turkey


Orcid: 0000-0002-2757-1802
Yazar: Suat TEKIN
Kurum: INONU UNIVERSITY
Ülke: Turkey


Orcid: 0000-0002-2321-1455
Yazar: Ömer KAYGILI
Kurum: FIRAT UNIVERSITY
Ülke: Turkey


Orcid: 0000-0001-7454-7605
Yazar: İsmail TURKOGLU
Kurum: FIRAT UNIVERSITY
Ülke: Turkey


Orcid: 0000-0002-7676-5953
Yazar: Ersin DEMIR
Kurum: DUZCE UNIVERSITY
Ülke: Turkey


Orcid: 0000-0001-6358-5913
Yazar: Mustafa KARATEPE
Kurum: FIRAT UNIVERSITY
Ülke: Turkey


Orcid: 0000-0002-4337-8236
Yazar: Sevda KIRBAG
Kurum: FIRAT UNIVERSITY
Ülke: Turkey


Orcid: 0000-0002-8276-4498
Yazar: Ökkes YILMAZ
Kurum: FIRAT UNIVERSITY
Ülke: Turkey


Orcid: 0000-0002-8916-3329
Yazar: Suleyman SANDAL
Kurum: INONU UNIVERSITY
Ülke: Turkey


Orcid: 0000-0001-7682-0513
Yazar: Semra TURKOGLU
Kurum: FIRAT UNIVERSITY

Destekleyen Kurum TÜBİTAK
Proje Numarası 114Z124
Teşekkür The TUBITAK was supported this study with under grant project number 114Z124.
Tarihler

Yayımlanma Tarihi : 31 Ocak 2020

Bibtex @araştırma makalesi { dubited595147, journal = {Düzce Üniversitesi Bilim ve Teknoloji Dergisi}, issn = {}, eissn = {2148-2446}, address = {}, publisher = {Düzce Üniversitesi}, year = {2020}, volume = {8}, pages = {693 - 701}, doi = {10.29130/dubited.595147}, title = {In vitro Antiradical, Antimicrobial and Antiproliferative Activities and Phytochemical Compositions of Endemic Alcea calvertii (Boiss) Boiss. Flowers}, key = {cite}, author = {Keser, Serhat and Keser, Fatma and Tekın, Suat and Kaygılı, Ömer and Turkoglu, İsmail and Demır, Ersin and Karatepe, Mustafa and Kırbag, Sevda and Yılmaz, Ökkes and Sandal, Suleyman and Turkoglu, Semra} }
APA Keser, S , Keser, F , Tekın, S , Kaygılı, Ö , Turkoglu, İ , Demır, E , Karatepe, M , Kırbag, S , Yılmaz, Ö , Sandal, S , Turkoglu, S . (2020). In vitro Antiradical, Antimicrobial and Antiproliferative Activities and Phytochemical Compositions of Endemic Alcea calvertii (Boiss) Boiss. Flowers . Düzce Üniversitesi Bilim ve Teknoloji Dergisi , 8 (1) , 693-701 . DOI: 10.29130/dubited.595147
MLA Keser, S , Keser, F , Tekın, S , Kaygılı, Ö , Turkoglu, İ , Demır, E , Karatepe, M , Kırbag, S , Yılmaz, Ö , Sandal, S , Turkoglu, S . "In vitro Antiradical, Antimicrobial and Antiproliferative Activities and Phytochemical Compositions of Endemic Alcea calvertii (Boiss) Boiss. Flowers" . Düzce Üniversitesi Bilim ve Teknoloji Dergisi 8 (2020 ): 693-701 <https://dergipark.org.tr/tr/pub/dubited/issue/49725/595147>
Chicago Keser, S , Keser, F , Tekın, S , Kaygılı, Ö , Turkoglu, İ , Demır, E , Karatepe, M , Kırbag, S , Yılmaz, Ö , Sandal, S , Turkoglu, S . "In vitro Antiradical, Antimicrobial and Antiproliferative Activities and Phytochemical Compositions of Endemic Alcea calvertii (Boiss) Boiss. Flowers". Düzce Üniversitesi Bilim ve Teknoloji Dergisi 8 (2020 ): 693-701
RIS TY - JOUR T1 - In vitro Antiradical, Antimicrobial and Antiproliferative Activities and Phytochemical Compositions of Endemic Alcea calvertii (Boiss) Boiss. Flowers AU - Serhat Keser , Fatma Keser , Suat Tekın , Ömer Kaygılı , İsmail Turkoglu , Ersin Demır , Mustafa Karatepe , Sevda Kırbag , Ökkes Yılmaz , Suleyman Sandal , Semra Turkoglu Y1 - 2020 PY - 2020 N1 - doi: 10.29130/dubited.595147 DO - 10.29130/dubited.595147 T2 - Düzce Üniversitesi Bilim ve Teknoloji Dergisi JF - Journal JO - JOR SP - 693 EP - 701 VL - 8 IS - 1 SN - -2148-2446 M3 - doi: 10.29130/dubited.595147 UR - https://doi.org/10.29130/dubited.595147 Y2 - 2019 ER -
EndNote %0 Düzce Üniversitesi Bilim ve Teknoloji Dergisi In vitro Antiradical, Antimicrobial and Antiproliferative Activities and Phytochemical Compositions of Endemic Alcea calvertii (Boiss) Boiss. Flowers %A Serhat Keser , Fatma Keser , Suat Tekın , Ömer Kaygılı , İsmail Turkoglu , Ersin Demır , Mustafa Karatepe , Sevda Kırbag , Ökkes Yılmaz , Suleyman Sandal , Semra Turkoglu %T In vitro Antiradical, Antimicrobial and Antiproliferative Activities and Phytochemical Compositions of Endemic Alcea calvertii (Boiss) Boiss. Flowers %D 2020 %J Düzce Üniversitesi Bilim ve Teknoloji Dergisi %P -2148-2446 %V 8 %N 1 %R doi: 10.29130/dubited.595147 %U 10.29130/dubited.595147
ISNAD Keser, Serhat , Keser, Fatma , Tekın, Suat , Kaygılı, Ömer , Turkoglu, İsmail , Demır, Ersin , Karatepe, Mustafa , Kırbag, Sevda , Yılmaz, Ökkes , Sandal, Suleyman , Turkoglu, Semra . "In vitro Antiradical, Antimicrobial and Antiproliferative Activities and Phytochemical Compositions of Endemic Alcea calvertii (Boiss) Boiss. Flowers". Düzce Üniversitesi Bilim ve Teknoloji Dergisi 8 / 1 (Ocak 2020): 693-701 . https://doi.org/10.29130/dubited.595147
AMA Keser S , Keser F , Tekın S , Kaygılı Ö , Turkoglu İ , Demır E , Karatepe M , Kırbag S , Yılmaz Ö , Sandal S , Turkoglu S . In vitro Antiradical, Antimicrobial and Antiproliferative Activities and Phytochemical Compositions of Endemic Alcea calvertii (Boiss) Boiss. Flowers. DÜBİTED. 2020; 8(1): 693-701.
Vancouver Keser S , Keser F , Tekın S , Kaygılı Ö , Turkoglu İ , Demır E , Karatepe M , Kırbag S , Yılmaz Ö , Sandal S , Turkoglu S . In vitro Antiradical, Antimicrobial and Antiproliferative Activities and Phytochemical Compositions of Endemic Alcea calvertii (Boiss) Boiss. Flowers. Düzce Üniversitesi Bilim ve Teknoloji Dergisi. 2020; 8(1): 693-701.