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Physicochemical and Antioxidant Responses of St. John’s Wort (Hypericum perforatum L.) under Drought Stress

Yıl 2021, Cilt: 9 Sayı: 1, 40 - 50, 31.01.2021

Hülya Torun Engin Eroğlu Vesile Yalçın Elmas Usta

https://doi.org/10.29130/dubited.847860

Öz

This study investigated the effects of drought stress on the physiological and biochemical responses of the medicinal and aromatic plant Hypericum perforatum (St. John’s Wort). Changes were determined in leaf length, relative water content (RWC), osmotic potential, chlorophyll fluorescence (Fv/Fm), lipid peroxidation (TBARS), hydrogen peroxide (H2O2), and proline content as well as in the antioxidant system enzyme activities of superoxide dismutase (SOD), catalase (CAT), peroxidase (POX), ascorbate peroxidase (APX), and glutathione reductase (GR). These responses were examined in relation to the tolerance of drought stress in H. perforatum. Ninety-day-old seedlings were subjected to drought for three weeks. The physiological parameters of leaf length, RWC, Fv/Fm, and osmotic potential were reduced under drought. The H2O2, TBARS, and proline levels were increased significantly under drought stress. Moreover, the proline content increase was greatly pronounced (25.9-fold) compared to the control groups. The high accumulation of proline may have resulted from the 83.8% leaf RWC still remaining under drought stress. On the other hand, the SOD, CAT, and GR enzyme activities were enhanced, whereas the POX and APX activities were reduced. The results indicate that improved tolerance to drought stress in H. perforatum plants may be accomplished through increased capacity of the antioxidative defense system

Anahtar Kelimeler

Antioxidant defense Drought stress Hypericum perforatum St. John’s wort

Destekleyen Kurum

Duzce University

Proje Numarası

2019.02.01.970

Teşekkür

This work was supported by funding from the Düzce University Research Foundation (Grant number 2019.02.01.970).

Kaynakça

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Kuraklık Stresi Altında Sarı Kantaronun (Hypericum perforatum L.) Fizikokimyasal ve Antioksidan Tepkileri

Yıl 2021, Cilt: 9 Sayı: 1, 40 - 50, 31.01.2021

Hülya Torun Engin Eroğlu Vesile Yalçın Elmas Usta

https://doi.org/10.29130/dubited.847860

Öz

Bu çalışmada, kuraklık stresinin Hypericum perforatum'daki (St. John's Wort) fizyolojik ve biyokimyasal tepkileri üzerine olan etkileri araştırılmıştır. Kuraklık stresine karşı toleransla ilişkili olarak bu tıbbi ve aromatik bitkide yaprak uzunluğu, bağıl su içeriği (RWC), ozmotik potansiyel, klorofil floresan (Fv/Fm), lipid peroksidasyonu (TBARS), hidrojen peroksit (H2O2), prolin içeriği ve antioksidan sistemdeki (süperoksit dismutaz (SOD), katalaz (CAT), peroksidaz (POX), askorbat peroksidaz (APX) ve glutatyon redüktaz (GR) enzim aktiviteleri) değişimler belirlenmiştir. Doksan günlük fidanlar 3 hafta süreyle kuraklığa maruz bırakılmıştır. Kuraklık altında fizyolojik parametrelerden uzunluk, RWC, Fv/Fm ve ozmotik potansiyel azalmıştır. Kuraklık stresi altında H2O2, TBARS ve prolin seviyeleri önemli ölçüde artmıştır; ancak kontrol grupları ile kıyaslandığında bu artış prolin içeriğinde (25.9 kat) daha belirgindir. Yüksek prolin birikimi, yapraktaki RWC'nin kuraklık stresi altında hala %83,8 olarak kalmasının bir sonucu olabilir. Diğer taraftan, SOD, CAT ve GR enzim aktiviteleri artarken, POX ve APX aktiviteleri azalmıştır. Sonuçlar, H. perforatum bitkisinde kuraklık stresine karşı geliştirilmiş toleransın, artan antioksidatif savunma sistemi kapasitesi ile başarılabileceğini göstermektedir.

Anahtar Kelimeler

Antioksidan savunma Kuraklık stresi Hypericum perforatum Sarı kantaron

Proje Numarası

2019.02.01.970

Kaynakça

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Toplam 90 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Mühendislik
Bölüm Makaleler
Yazarlar

Hülya Torun 0000-0002-1118-5130

Engin Eroğlu 0000-0002-1777-8375

Vesile Yalçın 0000-0003-1293-732X

Elmas Usta Bu kişi benim 0000-0001-8877-1106

Proje Numarası 2019.02.01.970
Yayımlanma Tarihi 31 Ocak 2021
Yayımlandığı Sayı Yıl 2021 Cilt: 9 Sayı: 1

Kaynak Göster

APA Torun, H., Eroğlu, E., Yalçın, V., Usta, E. (2021). Physicochemical and Antioxidant Responses of St. John’s Wort (Hypericum perforatum L.) under Drought Stress. Düzce Üniversitesi Bilim Ve Teknoloji Dergisi, 9(1), 40-50. https://doi.org/10.29130/dubited.847860
AMA Torun H, Eroğlu E, Yalçın V, Usta E. Physicochemical and Antioxidant Responses of St. John’s Wort (Hypericum perforatum L.) under Drought Stress. DÜBİTED. Ocak 2021;9(1):40-50. doi:10.29130/dubited.847860
Chicago Torun, Hülya, Engin Eroğlu, Vesile Yalçın, ve Elmas Usta. “Physicochemical and Antioxidant Responses of St. John’s Wort (Hypericum Perforatum L.) under Drought Stress”. Düzce Üniversitesi Bilim Ve Teknoloji Dergisi 9, sy. 1 (Ocak 2021): 40-50. https://doi.org/10.29130/dubited.847860.
EndNote Torun H, Eroğlu E, Yalçın V, Usta E (01 Ocak 2021) Physicochemical and Antioxidant Responses of St. John’s Wort (Hypericum perforatum L.) under Drought Stress. Düzce Üniversitesi Bilim ve Teknoloji Dergisi 9 1 40–50.
IEEE H. Torun, E. Eroğlu, V. Yalçın, ve E. Usta, “Physicochemical and Antioxidant Responses of St. John’s Wort (Hypericum perforatum L.) under Drought Stress”, DÜBİTED, c. 9, sy. 1, ss. 40–50, 2021, doi: 10.29130/dubited.847860.
ISNAD Torun, Hülya vd. “Physicochemical and Antioxidant Responses of St. John’s Wort (Hypericum Perforatum L.) under Drought Stress”. Düzce Üniversitesi Bilim ve Teknoloji Dergisi 9/1 (Ocak 2021), 40-50. https://doi.org/10.29130/dubited.847860.
JAMA Torun H, Eroğlu E, Yalçın V, Usta E. Physicochemical and Antioxidant Responses of St. John’s Wort (Hypericum perforatum L.) under Drought Stress. DÜBİTED. 2021;9:40–50.
MLA Torun, Hülya vd. “Physicochemical and Antioxidant Responses of St. John’s Wort (Hypericum Perforatum L.) under Drought Stress”. Düzce Üniversitesi Bilim Ve Teknoloji Dergisi, c. 9, sy. 1, 2021, ss. 40-50, doi:10.29130/dubited.847860.
Vancouver Torun H, Eroğlu E, Yalçın V, Usta E. Physicochemical and Antioxidant Responses of St. John’s Wort (Hypericum perforatum L.) under Drought Stress. DÜBİTED. 2021;9(1):40-5.
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