Influence of LED Lighting on Micropropagation of Alternanthera reineckii Briq. in Solid and Liquid Culture Media

Öz

The light is one of the important environmental factors in plant growth. Due to its many advantages, light emitting diodes (LEDs) have become one of the important light sources for regulating the light environment of plants. In this study, the effects of solid and liquid culture media containing Thidiazuron (TDZ) and different LED lighting conditions on the shoot regeneration ability of Alternanthera reineckii Briq. Nodal explants were cultured in Murashige and Skoog (MS) medium including 0.25-1 mg/L TDZ. Single red, blue and white LEDs and different combinations of red and blue LEDs are used for illumination. White fluorescent was used as a control. When the shoot regeneration values were examined, it was seen that LED lights were more efficient than fluorescent light. Higher shoot numbers and longer shoots were obtained in combinations of red and blue LED lights. 0.25 mg/L TDZ and 1R:2B LED light treatments gave the highest number of shoots in solid culture medium (9.03 shoots/explants). The maximum number of shoots in liquid culture medium was obtained in 0.50 mg/L TDZ and 2K:1M LED applications (11.62 shoots/explants). The longest shoots in solid and liquid cultures were determined under 1R:2B LED light and in MS nutrient medium with 0.25 mg/L TDZ. The plants rooted in tissue culture conditions were then successfully acclimatized to aquarium conditions.

Anahtar Kelimeler

• Nodal explant
• tissue culture
• in vitro propagation
• shoot regeneration

Katı ve Sıvı Kültür Ortamlarında LED Aydınlatmanın Alternanthera reineckii Briq.'nin Mikroçoğaltımı Üzerine Etkisi

Öz

Işık, bitki büyümesinde önemli çevresel faktörlerden biridir. Birçok avantajından dolayı ışık yayan diyotlar (LED), bitkilerin ışık ortamının düzenlenmesi için önemli ışık kaynaklarından biri haline gelmiştir. Bu çalışmada, Thidiazuron (TDZ) içeren katı ve sıvı kültür ortamları ve farklı LED aydınlatma koşullarının Alternanthera reineckii Briq.’nin sürgün rejenerasyon yeteneği üzerindeki etkileri incelenmiştir. Boğum eksplantları 0,25-1 mg/L TDZ içeren Murashige ve Skoog (MS) besin ortamında kültüre alınmıştır. Aydınlatma için kırmızı, mavi ve beyaz LED tek olarak ve kırmızı ve mavi LED ışıkların farklı kombinasyonları kullanılmıştır. Kontrol olarak beyaz floresan tercih edilmiştir. Sürgün rejenerasyon değerleri incelendiğinde, LED ışıkların flouresan ışığa göre daha verimli oldukları görülmüştür. Daha fazla sürgün sayısı ve uzun sürgünler kırmızı ve mavi LED ışıkların kombinasyonlarında elde edilmiştir. 0,25 mg/L TDZ ve 1K:2M LED ışık uygulamaları katı kültür ortamında en fazla sayıda sürgünleri vermiştir (9,03 sürgün/eksplant). Sıvı kültür ortamında en fazla sürgün sayısı 0,50 mg/L TDZ ve 2K:1M LED uygulamalarında elde edilmiştir (11,62 sürgün/eksplant). Katı ve sıvı kültürlerde en uzun sürgünler 1K:2M LED ışık altında ve 0,25 mg/L TDZ’li MS besin ortamında tespit edilmiştir. Doku kültürü koşullarında köklendirlen bitkiler ardından akvaryum koşullarına başarışı şekilde alıştırılmıştır.

Anahtar Kelimeler

• Boğum eksplant
• doku kültürü
• in vitro üretim
• sürgün rejenerasyonu

Kaynakça

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