Fırat Dikenli Yılan Balığı (Mastacembelus mastacembelus Banks & Solander, 1794) Böbreklerinin Histolojik Yapısı

Öz

Bu araştırmada; daha önce Fırat Üniversitesi Hayvan Deneyleri Yerel Etik Kurulu Başkanlığından onaylanmış olan 2014/113 protokol no’lu projeye ait olan Mastacembelus mastacembelus’dan alınan böbrek dokularına ait parafin bloklar kullanıldı. Bu parafin bloklardan 5-6 mikron kalınlığında alınan kesitlere hematoksilen eosin, Crosman's Mallory's Triple ve periodic acid shiff (PAS) boyamaları uygulandı. Boyamaları yapılan preparatlar ışık mikroskobunda incelendikten sonra mikrofotoğrafi ile görüntülenerek değerlendirilmeleri yapıldı. Mastacembelus mastacembelus’un böbreğinin, omurgaların altında, ince ve uzun iki lop şeklinde kırmızımsı kahverenginde ve ince bir zarla kaplı olduğu belirlendi. Böbreğin en küçük yapı birimi olan er nefronda glomerulus adı verien kılcal damar kütlesi bulunmaktadır ve glomerulusun etrafında aynı zamanda böbrek ünitesinin başlangıcı olan Bowman kapsulu yer alır. Ayrıca boyun segmenti, ardından glomerulusun çıkışından gelen süzülmüş sıvının ilk geçtiği bölüm olan birinci ve ikinci proksimal tubuller sıralanmaktadır. Proksimal tubulleri takiben geri emilim ve idrarın konsantre hale geldiği, distal tubuller ve distal tubullerden gelen idrarın toplandığı toplayıcı toplayıcı tubuller gözlendi.

Anahtar Kelimeler

• Mastacembelus mastacembelus
• böbrek
• histoloji

Histological Structure of Euphrates Spiny Eel (Mastacembelus mastacembelus Banks & Solander, 1794) Kidneys

Öz

In this study, paraffin blocks containing kidney tissues from Mastacembelus mastacembelus, which were obtained as part of project with protocol number 2014/113 approved by the Local Ethics Committee for Animal Experiments at Firat University, were used. Sections with a thickness of 5-6 microns were taken from these paraffin blocks and subjected to hematoxylin-eosin, Crosman's Mallory's Triple, and periodic acid Schiff (PAS) staining. After staining, the prepared specimens were examined under a light microscope, and evaluations were conducted by capturing microphotographs. It was determined that the kidney of Mastacembelus mastacembelus, situated beneath the vertebrae, is characterized by thin and elongated lobes with a reddish-brown color, covered by a delicate membrane. The smallest structural unit of the kidney, the renal corpuscle, contains a capillary mass called the glomerulus, and surrounding the glomerulus is the Bowman's capsule, marking the initiation of the renal unit. Additionally, the neck segment is followed by the first and second proximal tubules, where the filtered fluid from the glomerulus exit initially passes. Following the proximal tubules, there is reabsorption, leading to the concentration of urine, and distal tubules are observed follewed by collecting tubules where urine from the distal tubules is gathered.

Anahtar Kelimeler

• Mastacembelus mastacembelus
• kidney
• histology

Kaynakça

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