Determination of Oxidative Stress in Pleopodal Eggs of Propolis-Feeding Crayfish

Yıl 2023, Cilt: 6 Sayı: 2, 76 - 82, 31.12.2023

Serpil Mişe Yonar

https://doi.org/10.56728/dustad.1377353

Öz

Propolis is a sticky, resinous substance that has a unique smell and can vary in color from light brown to black, collected by bees from the leaves, buds, branches and bark of trees to protect themselves from cold and diseases. Propolis, an immunostimulant substance with properties such as antimicrobial, antibacterial, anticarcinogenic, antifungal, antiviral, antitumor, antiparasitic, antiprotozoan, anti-inflammatory, anesthetic, antioxidant and antiseptic, has attracted a lot of attention in recent years. In this study, changes in malondialdehyde (MDA) and glutathione (GSH) levels were investigated in pleopodal eggs taken from crayfish to which propolis was added to the feed at different rates. For this purpose, trial feeds containing 0% (control), 0.5% (D1), 1% (D2) and 2% (D3) propolis were created. At the end of 90 days of feeding, pleopodal egg samples were taken from crayfish. MDA and GSH levels were investigated in the samples taken. It was determined that MDA levels decreased in the pleopodal eggs of the propolis applied groups (p < 0.05). It was observed that GSH levels in pleopodal eggs increased (p < 0.05). It can be said that propolis has a protective effect against oxidative stress in crayfish eggs and can reduce stress during the reproductive period.

Anahtar Kelimeler

crayfish, glutathione, malondialdehyde, oxidative stress, propolis

Propolisle Beslenen Kerevitlerin Pleopodal Yumurtalarında Oksidatif Stresin Belirlenmesi

Öz

Propolis, arıların kendilerini soğuktan ve hastalıklardan korumak için ağaçların yaprak, tomurcuk, dal ve kabuklarından topladığı yapışkan, kendine özgü kokusu olan, açık kahverengiden siyaha kadar rengi değişebilen reçinemsi karakterdeki maddedir. Antimikrobiyal, antibakteriyel, antikanserojen, antifungal, antiviral, antitümör, antiparaziter, antiprotozoan, antiinflamatuar, anestetik, antioksidan, antiseptik gibi özelliklere sahip immünostimulan yapıda bir madde olan propolis son yıllarda oldukça fazla dikkat çekmektedir. Bu çalışmada, farklı oranlarda yeme katılan propolisin uygulandığı kerevitlerden alınan pleopodal yumurtalarda malondialdehit (MDA) ve glutatyon (GSH) düzeylerindeki değişimler araştırıldı. Bu amaçla % 0 (kontrol), % 0.5 (D1), % 1 (D2) ve % 2 (D3) oranlarında propolis içeren deneme yemleri oluşturuldu. 90 günlük besleme sonunda, kerevitlerden pleopodal yumurta örnekleri alındı. Alınan örneklerde MDA ve GSH düzeyleri araştırıldı. Propolis uygulanan grupların pleopodal yumurtalarında MDA düzeylerinin azaldığı belirlendi (p < 0, 05). Pleopodal yumurtalardaki GSH düzeylerinin ise arttığı (p < 0,05) gözlemlendi. Propolisin kerevit yumurtalarında oksidatif strese karşı koruyucu bir etki gösterdiği ve üreme döneminde stresi azaltabileceği söylenebilir.

Anahtar Kelimeler

Glutatyon, Kerevit, Malondialdehit, Oksidatif stres, Propolis

Kaynakça

• Bankova, V.S., Castro, De L.S., & Marcucci, M.C. (2000). Propolis: recent advances in chemistry and plant origin. Apidologie, 31, 3-15.
• Christov, R., Trusheva, B., Popova, M., Bankova, V., & Bertrand, M. (2006). Chemical composition of propolis from Canada, its antiradical activity and plant origin. Nat Prod Res., 20, 531-536.
• Ellman, G.L. 1(959). Tissue sulphydryl groups. Arch. Biochem. Biophys., 82, 70-77.
• Fontagné, S., Bazin, D., Brèque, J., Vachot, C., Bernarde, C., Rouault, T. … Bergot, P. (2006). Effects of dietary oxidized lipid and vitamin A on the early development and antioxidant status of Siberian sturgeon (Acipencer baeri) larvae. Aquaculture, 257, 400-411.
• Harlıoğlu, M.M. (2008). The harvest of the freshwater crayfish Astacus leptodactylus Eschscholtz in Turkey: harvest history, impact of crayfish plague, and present distribution of harvested populations. Aquaculture International, 16, 351-360.
• Harrlıoğlu, M.M., Köprücü, K., Yılmaz, Ö., Çakmak, M.N., Aksu, Ö., Harlıoğlu, A.G. … Özcan, S.( 2012). Kerevit yemine katılan n-3 serisi yağ asitlerinin pleopodal yumurta, hepatopankreas ve kas dokusunda lipid peroksidasyon ve glutatyon düzeylerine etkisi. Karadeniz Fen Bilimleri Dergisi, 2(5), 8-16.
• Hayes, J.D., & McLellan, L.I. (1999). Glutathione and glutathione-dependent enzymes represent a co-ordinately regulated defence against oxidative stres. Free Radical Research, 31, 273-300.
• Isla, M.I., Paredes-Guzman, J.F., Nieva-Moreno, M.I., Koo, H., & Park, Y.K. 2005. Some chemical composition and biological activity of northern Argentine propolis. J. Agric. Food. Chem., 53, 1166-1172.
• Kujumgiev, A., Tsvetkova, I., Serkedjieva, Y., Bankova, V., Christov, R., & Popov, S. (1999). Antibacterial, antifungal and antiviral activity of propolis of different geographic origin. J Ethnopharmacol, 64, 235-240.
• Lowry, O.H., Rosenberough, N.J., Farr, A.L., & Randal, R.J. (1951). Protein measurement with folinphenol reagent. J Biochem., 193, 265-275.
• Mani, F., Damasceno, H.C., Novelli, E.L., Martins, E.A., & Sforcin, J.M. (2006). Propolis: effect of different concentrations, extracts and intake period on seric biochemical variables. J. Ethnopharmacol., 105, 95-98.
• Marquele, F.D., Di Mambro, V.M., Georgetti, S.R., Casagrande, R., Valim, Y.M., & Fonseca, M.J. (2005). Assessment of the antioxidant activities of Brazilian extracts of propolis alone and in topical pharmaceutical formulations. J. Pharm. Biomed. Anal., 39, 455-462.
• Mişe Yonar, S. (2012). Kerevitin rasyonlarına ilave edilen selenyumun pleopodal yumurta ve birinci devre yavru sayısı ile oksidatif stres ve bazı antioksidan enzimler üzerine etkilerinin araştırılması. (Doktora Tezi) Fırat Üniversitesi Fen Bilimleri Enstitüsü.
• Mişe Yonar, S., Köprücü, K., Yonar, M.E., & Silici, S. (2017). Effects of dietary propolis on the number and size of pleopadal egg, oxidative stress and antioxidant status of freshwater crayfish (Astacus leptodactylus Eschscholtz). Animal Reproduction Science, 184, 149-159.
• Mişe Yonar, S., Ural, M.S., Silici, S., & Yonar, M.E. (2014). Malathion-induced changes in the haematological profile, the immune response, and the oxidative/antioxidant status of Cyprinus carpio carpio: protective role of propolis. Ecotoxicol. Environ. Saf., 102, 202-209.
• Morales, A.E., Pèrez-Jimènez, A., Hidalgo, M.C., Abellán, E., & Gabriel C.G. (2004). Oxidative stres and antioxidant defenses after prolonged starvation in Dentex dentex liver. Comporative Biochemistry and Physiology C, 139(1-3), 153-161.
• Placer, Z.A., Cushman, L., & Johnson, B.C. (1966). Estimation of products of lipid peroxidation (Malonyldialdehyde) in biological fluids. Anal. Biochem. 16, 359-364.
• Wang, L., Mineshita, S., Ga, L. (1993). Anti-inflammatory effects of propolis. Jpn J Pharmacol Therapeut 1993; 24, 223-226.
• Yonar, M.E., Mişe Yonar, S., & Silici, S. (2011). Protective effect of propolis against oxidative stress and immunosuppression induced by oxytetracycline in rainbow trout (Oncorhynchus mykiss, W.). Fish and Shellfish Immunology, 31, 318-325.