Using the MTT Cell Viability Test and Immunocytochemistry Techniques, Benzimidazole's Cytotoxicity and Apoptosis Activation in Mouse 4T1 Cell Culture

Abstract

Cancer is known as a major health problem globally. Breast cancer is the most common malignant tumor and metastasis continues to be the main cause of poor prognosis. Despite recent advances in cancer treatment, the success of metastatic breast cancer treatment is not at the desired level. Among the anticancer drugs discovered in recent years, various benzimidazole derivatives have attracted attention in the development of anticancer agents due to their various biological activities and clinical applications. The aim of this study was to evaluate the antiproliferative activity of synthesized benzimidazole derivative SA-61 in 4T1 breast cancer cell line in comparison with abemaciclib, which is approved by FDA for the treatment of breast cancer. The antiproliferative activity and apoptotic effect of SA-61 were examined using MTT and immunohistochemical methods, respectively. According to MTT results, compound SA-61 showed antiproliferative activity in 4T1 cells in a dose-dependent manner, but this effect was lower than abemaciclib. Although further studies are needed to specifically identify the compounds involved in its anti-cancer activity, our findings suggest that SA-61 inhibits the proliferation of 4T1 cells and its effect is dose dependent.

Keywords

• Benzimidazole
• Casp-3
• Cytotoxic activity
• 4T1 cell line.

Benzimidazolün Fare 4T1 Hücre Kültüründeki Apoptoz Aktivasyonunun ve Sitotoksisitesinin MTT Hücre Canlılık Testi ve İmmunsitokimya Yöntemleriyle Araştırılması

Öz

Kanser, küresel olarak önemli bir sağlık sorunu olarak bilinmektedir. Meme kanseri en yaygın kötü huylu tümördür ve metastaz kötü prognozun başlıca nedeni olmaya devam etmektedir. Son yıllarda kanser tedavisindeki gelişmelere rağmen, metastatik meme kanseri tedavisinin başarısı istenen düzeyde değildir. Son yıllarda keşfedilen antikanser ilaçları arasında, çeşitli biyolojik aktiviteleri ve klinik uygulamaları nedeniyle çeşitli benzimidazol türevleri antikanser ajanı geliştirmede dikkat çekmiştir. Bu çalışmanın amacı, 4T1 meme kanseri hücre hattında, sentezlenen benzimidazol türevi olan SA-61’in antiproliferatif aktivitesini, FDA tarafından meme kanseri tedavisi için onaylanan abemaciclib ile karşılaştırmalı olarak değerlendirmektir. SA-61’in antiproliferatif aktivitesi ve apoptotik etkisi sırasıyla MTT ve imunohistokimyasal yöntemler kullanılarak incelendi. MTT sonuçlarına göre SA-61 bileşiği 4T1 hücrelerinde doza bağlı bir şekilde antiproliferatif aktivite gösterdi fakat bu etki abemaciclibe kıyasla daha düşüktür. Kanser karşıtı aktivitesinde rol oynayan bileşiklerin spesifik olarak belirlenmesi için daha fazla çalışmaya ihtiyaç duyulmasına rağmen, bulgularımız SA-61’in 4T1 hücrelerinin çoğalmasını engellediğini ve etkisinin doza bağlı olduğunu göstermektedir.

Anahtar Kelimeler

• Benzimidazol
• Casp-3
• Sitotoksik aktivite
• 4T1 hücre hattı.

Kaynakça

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