Role of Neopestalotiopsis australis in Needle Necrosis Blight of Stone Pine Saplings in Türkiye

Deniz Cakar

doi:10.58816/duzceod.1472798

EN TR

Role of Neopestalotiopsis australis in Needle Necrosis Blight of Stone Pine Saplings in Türkiye

Öz

The stone pine (Pinus pinea L.) is a significant forest tree species in Türkiye. Its cultivation takes place in forest nurseries, and it is used in reforestation areas. The objective of this study to determine the causes of blight occurring in stone pine seedling in the Hendek Forest Nursery. Blight symptoms were observed on the needles of 1-2-years-old stone pine seedlings in the Hendek Forest Nursery. Isolates obtained as a result of isolation were identified as Neopestalotiopsis australis based on morphology and by combining sequence data from the LSU, ITS, tef1, and tub2 gene regions. A Maximum Likelihood phylogenetic tree was constructed for two isolates of N. australis based on four loci. Neopestalotiopsis australis was isolated from 25 out of 60 necrotic needles examined. The pathogenicity of N. australis was examined on healthy stone pine saplings by spraying spore suspension. After 28 days following the inoculation with spore suspensions, N. australis caused necrosis on needles. The pathogen was isolated from symptomatic needles in the stone pine the first time in the Türkiye. Neopestalotiopsis australis was proven to be a pathogen on stone pine. It has been concluded that N. australis could pose a threat to stone pine sapling. Further research should be conducted on this fungal pathogen for more detailed insights.

Anahtar Kelimeler

Stone pine, Fungus, Needle blight, Forest Nursery

Hendek Orman Fidanlığındaki Fıstıkçamı Fidanlarının İbre Kurumalarında Neopestalotiopsis australis’in Etkisi

Öz

Türkiye’de fıstık çamı (Pinus pinea L.) önemli bir orman ağacıdır. Orman fidanlıklarında üretimi yapılmakta ve ağaçlandırma sahalarında kullanılmaktadır. Bu çalışmanın amacı, Hendek Orman Fidanlığında fıstık çamı fidanlarında tespit edilen kurumaların nedenlerini belirlemektir. Hendek Orman Fidanlığında 1-2 yaşlı fıstık çamı fidanlarının ibrelerinde kurumalar görülmüştür. Morfolojik ve LSU, ITS, tef1 ve tub2 gen bölgelerinden elde edilen sekans verileri kombinlenerek, izolasyon sonucunda elde edilen izolatlar Neopestalotiopsis australis olarak tanılanmıştır. Neopestalotiopsis australis’in dört gen bölgesine dayalı iki izolatı için bir Maximum Likelihood filogenetik ağaç oluşturulmuştur. İncelenen 60 nekrotik ibrenin, 25 adedinden N. australis izole edilmiştir N. australis’in patojenitesi sağlıklı fıstıkçamı fidanlarına spor süspansiyonunu püskürtme yöntemi kullanılarak test edilmiştir. Spor süspansiyonu uygulamasından 28 gün sonra, N. australis ibrelerde nekrozlara neden olmuştur. Patojen, Türkiye’de fıstık çamı fidanlarında semptomatik ibrelerden ilk kez izole edilmişir. Fıstık çamı fidanlarında patojen olduğu tespit edilen Neopestalotiopsis australis’in ciddi bir tehdit oluşturabileceği öngörülmektedir. Gelecek çalışmalarda bu fungal etmenle ilgili daha detaylı araştırmalar yapılması önerilmektedir.

Anahtar Kelimeler

Fıstık çamı, Fungus, İbre kurumaları, Orman Fidanlığı

Teşekkür

I am thankful to the staff of the General Directory of Forestry and İsmail Yörük, The Chief of Nursery of Sakarya Forest Regional Directorate. I am also thankful to the helps of Dr. Seçil Akıllı Şimşek and Dr. Göksel Özer, who suggested valuable information on the current study.

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Orman Entomolojisi ve Orman Koruma

Bölüm

Araştırma Makalesi

Yazarlar

Deniz Cakar ^*
0000-0002-6269-404X
Türkiye

Yayımlanma Tarihi

28 Aralık 2024

Gönderilme Tarihi

24 Nisan 2024

Kabul Tarihi

11 Eylül 2024

Yayımlandığı Sayı

Yıl 2024 Cilt: 20 Sayı: 2

DOI

https://doi.org/10.58816/duzceod.1472798

IZ

https://izlik.org/JA56ZK86HM

APA

Cakar, D. (2024). Role of Neopestalotiopsis australis in Needle Necrosis Blight of Stone Pine Saplings in Türkiye. Düzce Üniversitesi Orman Fakültesi Ormancılık Dergisi, 20(2), 160-176. https://doi.org/10.58816/duzceod.1472798

AMA

1.Cakar D. Role of Neopestalotiopsis australis in Needle Necrosis Blight of Stone Pine Saplings in Türkiye. DÜOD. 2024;20(2):160-176. doi:10.58816/duzceod.1472798

Chicago

Cakar, Deniz. 2024. “Role of Neopestalotiopsis australis in Needle Necrosis Blight of Stone Pine Saplings in Türkiye”. Düzce Üniversitesi Orman Fakültesi Ormancılık Dergisi 20 (2): 160-76. https://doi.org/10.58816/duzceod.1472798.

EndNote

Cakar D (01 Aralık 2024) Role of Neopestalotiopsis australis in Needle Necrosis Blight of Stone Pine Saplings in Türkiye. Düzce Üniversitesi Orman Fakültesi Ormancılık Dergisi 20 2 160–176.

IEEE

[1]D. Cakar, “Role of Neopestalotiopsis australis in Needle Necrosis Blight of Stone Pine Saplings in Türkiye”, DÜOD, c. 20, sy 2, ss. 160–176, Ara. 2024, doi: 10.58816/duzceod.1472798.

ISNAD

Cakar, Deniz. “Role of Neopestalotiopsis australis in Needle Necrosis Blight of Stone Pine Saplings in Türkiye”. Düzce Üniversitesi Orman Fakültesi Ormancılık Dergisi 20/2 (01 Aralık 2024): 160-176. https://doi.org/10.58816/duzceod.1472798.

JAMA

1.Cakar D. Role of Neopestalotiopsis australis in Needle Necrosis Blight of Stone Pine Saplings in Türkiye. DÜOD. 2024;20:160–176.

MLA

Cakar, Deniz. “Role of Neopestalotiopsis australis in Needle Necrosis Blight of Stone Pine Saplings in Türkiye”. Düzce Üniversitesi Orman Fakültesi Ormancılık Dergisi, c. 20, sy 2, Aralık 2024, ss. 160-76, doi:10.58816/duzceod.1472798.

Vancouver

1.Deniz Cakar. Role of Neopestalotiopsis australis in Needle Necrosis Blight of Stone Pine Saplings in Türkiye. DÜOD. 01 Aralık 2024;20(2):160-76. doi:10.58816/duzceod.1472798

DÜOD'da yayımlanan makaleler Creative Commons Atıf-GayriTicari 4.0 (CC BY-NC) kapsamında lisanslanmıştır.

Role of Neopestalotiopsis australis in Needle Necrosis Blight of Stone Pine Saplings in Türkiye

Öz

Anahtar Kelimeler

Hendek Orman Fidanlığındaki Fıstıkçamı Fidanlarının İbre Kurumalarında Neopestalotiopsis australis’in Etkisi

Öz

Anahtar Kelimeler

Teşekkür

Kaynakça

Ayrıntılar

Birincil Dil

Konular

Bölüm

Yazarlar

Yayımlanma Tarihi

Gönderilme Tarihi

Kabul Tarihi

Yayımlandığı Sayı

DOI

IZ

Kaynak Göster