Kuraklığın Ebegümeci (Malva sylvestris L.) Bitkisinin Su Durumu, Büyümesi ve Antioksidan Sistem Üzerine Etkisi

Yıl 2024, Cilt: 20 Sayı: 2, 28 - 40, 28.12.2024

Seda Şahin Hülya Torun

https://doi.org/10.58816/duzceod.1598233

Öz

Ebegümeci (Malva sylvestris L.), Malvaceae familyasına üye, çiçekleri mor çizgili pembe renkli, tıbbi aromatik bir bitki olup gıda ve sağlık alanlarında oldukça yaygın kullanılmaktadır. Bu çalışmanın amacı, kuraklık stresinin ebegümeci bitkisinin büyümesi, su içeriği ve antioksidan savunma sistemi enzimleri üzerine etkilerini incelemektir. Bu doğrultuda, 21 gün kuraklığa maruz bırakılan bitkinin yapraklarında nisbi büyüme, osmotik potansiyel, nisbi su içeriği, lipid peroksidasyonu, hidrojen peroksit içeriği ile süperoksit dismutaz (SOD), peroksidaz (POX), katalaz (CAT), askorbat peroksidaz (APX) ve glutatyon redüktaz (GR) antioksidan enzim aktiviteleri ölçülmüştür. Kuraklık stresi altındaki bitkilerle stres uygulaması olmayan bitkiler kıyaslandığında, büyüme oranı, nisbi su içeriği, osmotik potansiyel önemli ölçüde azalmıştır. Ayrıca, lipid peroksidasyonu ve hidrojen peroksit miktarları kuraklık uygulaması ile artmıştır. Bitkinin savunma sistemi kuraklık stresi altında uyarılmış ve SOD, POX, CAT, APX ve GR aktivitelerinde artış kaydedilmiştir. Bu çalışmada, kuraklık stresine maruz kalan ebgümecinde antioksidan savunma sisteminin etkinliği belirlenmiştir.

Anahtar Kelimeler

Antioksidan enzimler, Ebegümeci, Kuraklık stresi, Malva sylvestris

Etik Beyan

Gerek yoktur

Effect of Drought on Water Status, Growth and Antioxidant System of Mallow (Malva sylvestris L.)

Öz

Mallow (Malva sylvestris L.) is a member of the Malvaceae family, a medicinal aromatic plant with pink flowers with purple stripes and is widely used in food and health fields. The aim of this study was to investigate the effects of drought stress on growth, water content and antioxidant defence system enzymes of mallow. Accordingly, relative Growth rate, osmotic potential, relative water content, lipid peroxidation, hydrogen peroxide content and antioxidant enzyme activities of superoxide dismutase (SOD), peroxidase (POX), catalase (CAT), ascorbate peroxidase (APX) and glutathione reductase (GR) were measured in the leaves of plants exposed to drought for 21 days. When plants under drought stress were compared with non-stressed plants, growth rate, relative water content and osmotic potential were significantly decreased. In addition, lipid peroxidation and hydrogen peroxide levels increased with drought stress. Plant defence system was stimulated under drought stress and SOD, POX, CAT, APX and GR activities were increased. In this study, the efficiency of antioxidant defence system in mallow exposed to drought stress was determined.

Anahtar Kelimeler

Antioxidant enzymes, Drought stress, Mallow, Malva sylvestris

Kaynakça

• Abbas, G., Murtaza, B., Amjad, M., Saqib, M., Akram, M., Naeem, M. A., & Ahmed, K. (2024). Heat stress resulting from late sowing impairs grain yield and quality of quinoa genotypes facing drought and salt stress under field conditions. Journal of Agronomy and Crop Science, 210(4), e12717.
• Abdalla, M., Ahmed, M. A., Cai, G., Wankmüller, F., Schwartz, N., Litig, O., Javaux, M., & Carminati, A. (2022). Stomatal closure during water deficit is controlled by below-ground hydraulics. Annals of Botany, 129, 161-170.
• Aebi, H. (1984). Catalase in vitro. In: Methods in Enzymology. (eds) Colowick. S. P., Kaplan, N. O., Orlando: Academic Press, 114-121.
• Agriculture| UN World Water Development Report. (2022). https://www.unesco.org/reports/wwdr/2022/en/agriculture. (Erişim Tarihi: 21.11.2024).
• Ahmad, Z., Anjum, S., Waraich, E. A., Ayub, M. A., Ahmad, T., Tariq, R. M. S., Ahmad, R., & Iqbal, M. A. (2018). Growth, physiology, and biochemical activities of plant responses with foliar potassium application under drought stress – A review. Journal of Plant Nutrition, 41, 1734-1743.
• Ahanger, M. A., & Agarwal, R. M. (2017). Potassium up-regulates antioxidant metabolism and alleviates growth inhibition under water and osmotic stress in wheat (Triticum aestivum L). Protoplasma, 254, 1471-1486.
• Akash, M. S. H., Rehman, K., & Chen, S. (2013). Role of inflammatory mechanisms in pathogenesis of type 2 diabetes mellitus. Journal of Cellular Biochemistry, 114, 525-531.
• Akash, M. S. H., Shen, Q., & Rehman, K. (2012). Interleukin-1 receptor antagonist: a new therapy for type 2 diabetes mellitus. Journal of Pharmaceutical Sciences, 101, 1647-1658.
• Atta, K., Sen, J., Chettri, P., & Pal, A. K. (2022). Antioxidant responses of ricebean [Vigna umbellata (Thunb.) Ohwi and Ohashi] seedling under iso-osmotic potential of salinity and drought stress. Legume Research-An International Journal, 45(4), 429-434.
• Aydın, S. S., Gökçe, E., Büyük, İ., & Aras, S. (2012). Characterization of stress induced by copper and zinc on cucumber (Cucumis sativus L.) seedlings by means of molecular and population parameters. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 746(1), 49-55.
• Bayar, E., & Deligöz, A. (2019). Cedrus libani ve Pinus nigra subsp. pallasiana ağaçlandırma alanında kurak dönemde ağaç-su ilişkisi değişimleri. Türkiye Ormancılık Dergisi, 20(4), 317-323.
• Bailey-Serres, J., & Mittler, R. (2006). The roles of reactive oxygen species in plant cells. Plant Physiology, 141(2), 311.
• Barzotto, G. R., Cardoso, C. P., Jorge, L. G., Campos, F. G., & Boaro, C. S. F. (2023). Hydrogen peroxide signal photosynthetic acclimation of Solanum lycopersicum L. cv Micro-Tom under water deficit. Scientific Reports, 13, 13059.
• Beauchamp, C., & Fridovich, I. (1971). Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Analytical Biochemistry, 44, 276-287.
• Bettaieb, I., Hamrouni Sellami, I., Bourgou, S., Limam, F., & Marzouk, B. (2011). Drought effects on polyphenol composition and antioxidant activities in aerial parts of Salvia officinalis L. Acta Physiologiae Plantarum, 33(4), 1103-1111.
• Bradford, M. M. (1976). A rapid and sensitive method for the quantization of microgram quantities of protein utilizing the principle of the protein-dye binding. Analytical Biochemistry, 72, 248–254.
• Bijanzadeh, E., Naderi, R., & Barati, V. (2023). Water uptake, anatomical, and biochemical changes of sorghum genotypes subjected to polyethylene glycol-induced drought stress. Arid Land Research and Management, 37(4), 554-576.
• Doyotte, A., Cossu, C., Jacquin, M.C., Babutb, M., & Vaseural, P. (1997). Antioxidant enzymes, glutathione and lipid peroxidation as relevant biomarkers of experimental or field exposure in the gills and the digestive gland of the freshwater bivalve unio tumidus. Aquatic Toxicology, 39, 93-110
• Dündar, Y., & Aslan. R. (2000). Hekimlikte oksidatif stres ve antioksidanlar. Afyon Kocatepe Üniversitesi Yayınları, Afyonkarahisar.
• Dvojković, K., Plavšin, I., Novoselović, D., Šimić, G., Lalić, A., Čupić, T., Horvat, D., & Viljevac Vuletić, M. (2023). Early antioxidative response to desiccant-stimulated drought stress in field-grown traditional wheat varieties. Plants (Basel), 12(2), 249.
• Elbasan, F. (2019). ‘Çoklu streslere maruz bırakılan çeltik (Oryza sativa L.) yapraklarında eksojen skandiyum (sc)'un reaktif oksijen türleri (ROS) ve antioksidan savunma sistemi üzerine etkileri’. Yüksek Lisans Tezi, Selçuk Üniversitesi, Fen Bilimleri Enstitüsü, Konya.
• Foyer, C. H., & Halliwell, B. (1976). The presence of glutathione and glutathione reductase in chloroplasts: A proposed role in ascorbic acid metabolism. Planta, 133, 21–25.
• Fukase, E., & Martin, W. (2020) Economic growth, convergence, and world food demand and supply. World Development, 132, 104954.
• Gitz, V., Meybeck, A., Lipper, L., Young, C. D., & Braatz, S. (2016). Climate Change and Food security: Risks and Responses. Food and Agriculture Organization of the United Nations (FAO) Report.
• Greve, P., Orlowsky, B., Mueller, B., Sheffield, J., Reichstein, M., & Seneviratne, S. I. (2014). Global assessment of trends in wetting and drying over land. Nature Geoscience, 7, 716–721.
• Hasanuzzaman, M., & Fujita, M. (2022). Plant oxidative stress: biology, physiology and mitigation. Plants (Basel), 11(9), 1185.
• Hideg, E., Jansen, M. A. K., & Strid, A. (2013). UV-B exposure, ROS, and stress: Inseparable companions or loosely linked associates? Trends in Plant Science, 18, 107-115.
• Hunt, R., Causton, D. R., Shipley, B., & Askew, A. P. (2002). A modern tool for classical plant growth analysis. Annals of Botany, 90, 485-488.
• Hura, T., Hura, K., & Ostrowska, A. (2022). Drought-stress induced physiological and molecular changes in plants. International Journal of Molecular Sciences, 23(9), 4698.
• Hussain, H. A., Hussain, S., Khaliq, A., Ashraf, U., Anjum, S. A., Men, S., & Wang. L. (2018). Chilling and drought stresses in crop plants: implications, cross talk, and potential management opportunities. Frontiers in Plant Science, 9, 393.
• Lee, S., & Park, C. M. (2012). Regulation of reactive oxygen species generation under drought conditions in Arabidopsis. Plant Signaling & Behavior, 7(6), 599-601.
• Liu, J., Lu, B., & Xun, A. L. (2000). An improved method for the determination of hydrogen peroxide in leaves. Progress in Biochemistry and Biophysics, 27, 548–551.
• Madhava-Rao, K. V., & Sresty, T. V. S. (2000). Antioxidative parameters in the seedlings of pigeonpea (Cajanus cajan L. Millspaugh) in response to Zn and Ni stresses. Plant Science, 157, 113-128.
• Marouane, W., Soussi, A., & Murat, J. C. (2011). The protective effect of Malva sylvestris on rat kidney damaged by vanadium. Lipids in Health and Disease, 10, 65.
• Mazhar, M. W., Ishtiaq, M., Maqbool, M., Ullah, F., Sayed, S. R., & Mahmoud, E. A. (2023). Seed priming with iron oxide nanoparticles improves yield and antioxidant status of garden pea (Pisum sativum L.) grown under drought stress. South African Journal of Botany, 162, 577-587.
• Mika, A., & Lüthje, S. (2003). Properties of guaiacol peroxidase activities isolated from corn root plasma membranes. Plant Physiology, 132, 1489–1498.
• Mustafa, A., & Ali, M. (2011). New steroidal lactones and homomonoterpenic glucoside from fruits of Malva sylvestris L. Acta Poloniae Pharmaceutica, 68(3), 393–401.
• Nakano, Y., & Asada, K. (1981). Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplasts. Plant and Cell Physiology, 22, 867–880.
• Nawaz, M., Sun, J., Shabbir, S., Khattak, W. A., Ren, G., Nie, X., Bo, Y., Javed, Q., Du, D., & Sonne, C. (2023). A review of plants strategies to resist biotic and abiotic environmental stressors. Science of The Total Environment, 900, 65832.
• Newman, D. J., & Cragg, G. M. (2016). Natural products as sources of new drugs over the period 1981-2014. Journal of Natural Products, 79(3), 629-661.
• Osakabe, Y., Osakabe, K., Shinozaki, K., & Tran, L. S. P. (2014). Response of plants to water stress. Frontiers in Plant Science, 5, 86.
• Rady, M. M., Belal, H. E. E., Gadallah, F. M., & Semida, W. M. (2020). Selenium application in two methods promotes drought tolerance in Solanum lycopersicum plant by inducing the antioxidant defense system. Scientia Horticulturae, 266, 109290.
• Ramakrishna, A., & Ravishankar, G. A. (2011). Influence of abiotic stress signals on secondary metabolites in plants. Plant Signaling & Behavior, 6, 720-1731.
• Raza, A., Razzaq, A., Mehmood, S. S., Zou, X., Zhang, X., Lv, Y., & Xu, J. (2019). Impact of climate change on crops adaptation and strategies to tackle its outcome: A Review. Plants, 8, 34.
• Razavi, S. M., Zarrini, G., & Molavi, G. (2011). Bioactivity of Malva sylvestris L., a medicinal plant from Iran. Iranian Journal of Basic Medical Sciences, 14, 574-579.
• Rojas, O. (2020). Agricultural extreme drought assessment at global level using FAO-Agricultural Stress Index System (ASIS). Weather and Climate Extremes, 27, 100184.
• Sabir, S. M., & Rocha, J. B. T. (2008). Water-extractable phytochemicals from Phyllanthus niruri exhibit distinct in vitro antioxidant and in vivo hepatoprotective activity against paracetamol-induced liver damage in mice. Food Chemistry, 111, 845–851.
• Santa-Cruz, A., Martinez-Rodriguez, M. M., Perez-Alfocea, F., Romero-Aranda, R., & Bolarin M.C. (2002). The rootstock effect on the tomato salinity response depends on the shoot genotype. Plant Science, 162, 825–831.
• Shahimoghadam, M., Asghari, A., Moharramnejad, S., Dehghanian, Z., Singh, S. K., Sivalingam, K. M., & Marisennayya, S. (2024). Variation in oxidative defense system and physiological traits in maize under drought stress. Plant Science Today, 11(2).
• Smart, R. E., & Bingham, G.E. (1974). Rapid estimates of relative water content. Plant Physiology, 53, 258-260.
• Suhail, F., Afzal, A., Naseer, L., Pervaiz, A., Ikram, M., Shaheen, S., & Khan, N. (2023). Influence of phosphate solubilizing bacteria on the growth of mustard grown under drought stress conditions. Agricultural Research, 12(4), 375-386.
• Tabaraki, R., Yosefi, Z., & Asadi Gharneh, H. A. (2012). Chemical composition and antioxidant properties of Malva sylvestris L. Journal of Research in Agricultural Science, 8(1), 59-68.
• Teker, M. (2017). ‘Mercimek bitkisi Orobanche crenata Forsk. interaksiyonunda eksojen glisin betainin antioksidan savunma sistemi üzerine etkisi’. Yüksek Lisans Tezi, Çanakkale Onsekiz Mart Üniversitesi, Fen Bilimleri Enstitüsü, Çanakkale.
• Tian, H., Zhou, Q., Liu, W., Zhang, J., Chen, Y., Jia, Z., & Wang, H. (2022). Responses of photosynthetic characteristics of oat flag leaf and spike to drought stress. Frontiers in Plant Science, 13, 917528.
• Upadhyay, D., Budhlakoti, N., Singh, A. K., Bansal, R., Kumari, J., Chaudhary, N., Padaria, J. C., Sareen, S., & Kumar, S. (2020). Drought tolerance in Triticum aestivum L. genotypes associated with enhanced antioxidative protection and declined lipid peroxidation. BioTech, 10(6), 281.
• Xiao, M., Yu, Z., Kong, D., Gu, X., Mammarella, I., & Montagnani, L. (2020). Stomatal response to decreased relative humidity constrains the acceleration of terrestrial evapotranspiration. Environmental Research Letters, 15, 094066.
• Zhang, H., Zhao, Y., & Zhu, J. K. (2020). Thriving under stress: how plants balance growth and the stress response. Developmental Cell, 55(5), 529-543.