Effects of Metoprolol on Experimental Spinal Cord Ischemia-Reperfusion Injury in Rats

Öz

Aim: The aim of this study was to investigate the neuroprotective effect of metoprolol and its efficacy in reducing lipid peroxidation levels in the spinal cord ischemia-reperfusion model in rats. Material and Methods: Twenty (20) Sprague-Dawley female rats weighing between 220 gr and 280 gr were randomly divided into 3 groups. Only laparotomy was performed in the control group, and the aorta abdominalis was revealed. In the groups other than the control group, clip compression was applied to the aorta abdominalis for 45 minutes. The ischemia group was not given any medication. Metoprolol was administered intraperitoneally at 0.5 mg/kg to the metoprolol group. Motor examination was made according to Tarlov scale at the 1st and 24th hours and then, spinal cords of all rat models were removed. Spinal cord tissue samples were collected for histopathological examination and for determining malondialdehyde (MDA) level. All rats were sacrificed by draining blood after their motor examinations. Results: According to motor examination findings at the 1st and 24th hours, metoprolol resulted in a statistically significant improvement in recovery (p=0.045). Histopathological examinations revealed that metoprolol contributed to neurological recovery by reducing neuronal necrosis. MDA levels, which is an indicator of lipid peroxidation, were significantly lower in the metoprolol group when compared to the ischemia group (p=0.001). Conclusion: Metoprolol was found to be significantly effective in reducing and/or preventing spinal cord ischemia-reperfusion injury.

Anahtar Kelimeler

• beta-blocker
• ischemia/reperfusion injury
• metoprolol
• metoprolol
• metoprolol
• metoprolol
• metoprolol
• metoprolol
• metoprolol
• metoprolol
• metoprolol

Sıçanlarda Metoprololün Deneysel Omurilik İskemisi/Reperfüzyon Hasarı Üzerine Etkileri

Öz

Amaç: Bu çalışmanın amacı, metoprololün nöroprotektif etkisini ve sıçanlarda omurilik iskemisi/reperfüzyon modelinde lipid peroksidasyon düzeylerini azaltmadaki etkinliğini araştırmaktır. Gereç ve Yöntemler: 220 gr ve 280 gr ağırlığında yirmi (20) Sprague-Dawley dişi sıçan rastgele 3 gruba ayrıldı. Kontrol grubunda sadece laparotomi yapıldı ve aort abdominalis ortaya konuldu. Kontrol grubu dışındaki gruplarda, aort abdominalise 45 dakika boyunca klips kompresyon uygulandı. İskemi grubuna herhangi bir ilaç verilmedi. Metoprolol grubuna intraperitonal olarak 0,5 mg/kg metoprolol uygulandı. Motor muayene 1. ve 24. saatlerde Tarlov ölçeğine göre yapıldı ve daha sonra tüm sıçanların omurilikleri çıkarıldı. Histopatolojik inceleme ve malondialdehit (MDA) seviyesini belirlemek için omurilik doku örnekleri toplandı. Tüm sıçanlar motor muayenelerinden sonra kansızlaştırma yoluyla sakrifiye edildi. Bulgular: 1. ve 24. saatteki motor muayene bulgularına göre, metoprolol istatistiksel olarak anlamlı bir iyileşme sağladı (p=0,045). Histopatolojik incelemeler, metoprololün nöronal nekrozu azaltarak nörolojik iyileşmeye katkıda bulunduğunu ortaya koydu. Lipid peroksidasyonunun bir göstergesi olan MDA düzeyleri, metoprolol grubunda iskemi grubuna göre anlamlı olarak daha düşüktü (p=0,001). Sonuç: Metoprololün omurilik iskemi/reperfüzyon hasarını azaltmada ve/veya önlemede önemli ölçüde etkili olduğu ortaya konulmuştur.

Anahtar Kelimeler

• İskemi-reperfüzyon hasarı
• omurilik
• metoprolol

Kaynakça

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