Salvia verticillata subsp. verticillata Bitkisinin Fungal Patojenlerine Karşı Antifungal Aktivitesi

Öz

Amaç: Bu çalışmada, Lamiaceae familyasından Salvia verticillata subsp. verticillata L. (Lamiaceae) bitkisinden elde edilen yaprak, kök ve kombine (yaprak+kök) ekstresinin antifungal aktivitesinin araştırılması amaçlandı. Gereç ve Yöntemler: Bitki örnekleri, Düzce ili, Samandere köyü civarında haziran ayında çiçeklenme döneminde toplanmıştır. Toplanan bitkiler herbaryum tekniklerine göre kurutuldu. Toz haline getirilmiş bitki materyalinin, etil alkol (96%) kullanılarak ekstraksiyonu yapıldı. Tıbbi maya kültürleri (Candida krusei ATCC 20298, C. parapsilosis ATCC 90112, C. guilliermondii ATCC 6260, C. albicans ATCC 10231, C. tropicalis ATCC 13808, C. glabrata ATCC 2001, Cryptococcus laurentii ATCC 34142, C. neoformans ATCC 90112, Geotrichum capitatum ATCC 28576, Debaryomyces hansenii DSM 70238, Rhodotorula rubra DSM 70403 ve Kluyveromyces fragilis ATCC 8608) kullanılarak, görsel sıvı mikrodilüsyon yöntemi ile değerlendirildi. Bulgular: Çalışmada sonuçları karşılaştırmak için antifungal antibiyotik ketokonazol kullanıldı. MİK değerlerinin 1.56 ile 25.00 mg / mL arasında değiştiği saptandı. Ekstreler, maya kültürlerine karşı güçlü antifungal etki sergilemiştir. Ancak bitki (yaprak+kök) ekstre kombinasyonu, Cryptococcus neoformans, C. laurentii ve Geotrichum capitatum maya kültürlerine karşı daha güçlü antifungal etkiye sahip olduğu belirlenmiştir. Sonuç: Sonuçlarımız, Salvia verticillata subsp. verticillata ekstrelerinin mantar enfeksiyonlarının tedavisi için geleneksel tıpta kullanımını destekler niteliktedir.

Anahtar Kelimeler

• Salvia verticillata
• bitki ekstreleri
• antifungal aktivite

Antifungal activity of Salvia verticillata subsp. verticillata against fungal pathogens

Öz

Aim: It was aimed to investigate the antifungal activity of the individual as well as the combine extract (leaf+root) of Salvia verticillata subsp. verticillata L. from Lamiaceae family which has ethnobotanical use. Material and Methods: The plant samples were collected during to flowering stage in June, Samandere village around, Duzce, Turkey. The collected plants were dried according to herbarium techniques. The powdered plant material was extraction using ethyl alcohol (96%). Antifungal effects of the extracts was evaluated for yeasts (Candida krusei ATCC 20298, C. parapsilosis ATCC 90112, C. guilliermondii ATCC 6260, C. albicans ATCC 10231, C. tropicalis ATCC 13808, C. glabrata ATCC 2001, Cryptococcus laurentii ATCC 34142, C. neoformans ATCC 90112, Geotrichum capitatum ATCC 28576, Debaryomyces hansenii DSM 70238, Rhodotorula rubra DSM 70403, and Kluyveromyces fragilis ATCC 8608) by visual broth microdilution method. Antifungal antibiotic ketoconazole was used in the study to compare the results. Results: MIC values ranged from 1.56 to 25.00 mg/mL. The extracts have shown potential antifungal effects against the yeast cultures. Notably, the combine plant extracts (both leaves and rootstock) possessed higher antifungal potency against C. neoformans, C. laurentii and G. capitatum. Conclusion: Our results demonstrated that the extracts of Salvia verticillata subsp. verticillata are used for the treatment of fungal infections as traditional medicine

Anahtar Kelimeler

• Salvia verticillata
• plant extracts
• antifungal activity

Kaynakça

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