Effects of Aerobic Exercise on Oxidant/Antioxidant Status in Obese Boys: A Controlled Trial

Öz

It is unclear how aerobic exercise program effects oxidative stress and antioxidant defense parameters in obese children; therefore, our objective is to examine the effects of aerobic exercise on parameters of oxidative stress and antioxidant in obese boys. Our study included 10 obese boys, aged from 13 to 15, and 10 healthy boys as control group. Before and after exercise, 10 ml blood specimens were taken from the obese boys, who exercised 3 days/week for 12 weeks. 10 ml blood specimens were also taken from the health control group. The oxidative stress and antioxidant defense parameter levels in blood samples were measured for both groups. In our study, it was found that control group and obese children after aerobic exercise had significantly higher superoxide dismutase, catalase, glutathione peroxidase and paraoxonase-1 values than those of obese boys before aerobic exercise. On the other hand, it was found that after aerobic exercise, control group and obese children had significantly lower malondialdehyde, nitric oxide and carbonyl values than those of obese boys before aerobic exercise. When these findings are taken into account, it may be suggested that aerobic exercise regulated oxidative stress and improved the state of antioxidant status in obese boys.

Anahtar Kelimeler

• Childhood obesity
• Oxidative stress
• Antioxidant
• Regular exercises
• Carbonyl

Kaynakça

1. 1. Lm T, Or T. Pre and post-natal risk and determination of factors for child obesity. J Med Life. 2016;9:386-91.
2. 2. Fernández-Sánchez A, Madrigal-Santillán E, Bautista M, et al. Inflammation, oxidative stress, and obesity. Int J Mol Sci. 2011;12;3117-32.
3. 3. Yelinova V, Glazachev Y, Khramtsov V, Kudryashova L, Rykova V, Salganik R. Studies of human and rat blood under oxidative stress: changes in plasma thiol level, antioxidant enzyme activity, protein carbonyl content, and fluidity of erythrocyte membrane. Biochem Biophys Res Commun. 1996;221:300-3.
4. 4. Tomás M, Elosua R, Sentí M, et al. PON1-192 polymorphism modulates the effects of regular and acute exercise on paraoxonase1 activity. J Lipid Res. 2002;43:713-20.
5. 5. Fisher-Wellman K, Bell HK, Bloomer RJ. Oxidative stress and antioxidant defense mechanisms linked to exercise during cardiopulmonary and metabolicdisorders. Oxid Med Cell Longev. 2009;2:43-51.
6. 6. Steinbacher P, Eckl P. Impact of oxidative stress on exercising skeletalmuscle. Biomolecules. 2015;5:356-77.
7. 7. Strijdom H, Chamane N, Lochner A. Nitric oxide in the cardiovascular system: a simple molecule with complex actions. Cardiovasc J Afr. 2009;20:303-10.
8. 8. Kruk J, Duchnik E. Oxidative stress and skin diseases: possible role of physical activity. Asian Pac J Cancer Prev. 2014;15:561–8.

9. 9. Ruiz-Extremera Á, Carazo Á, Salmerón Á, et al. Factors associated with hepatic steatosis in obese children and adolescents. J Pediatr Gastroenterol Nutr. 2011;53:196-201.
10. 10. Julious SA. Sample sizes for clinical trials with normal data. Stat Med. 2004;23:1921-86.
11. 11. Bundak R, Furman A, Gunoz H, Darendeliler F, Bas F, Neyzi O. Body mass index references for Turkish children. Acta Paediatr. 2006;95:194-8.
12. 12. Özer K. Physical Fitness, 2nd Edition, Nobel Offset, Ankara, 2006.
13. 13. Ignaszewski M, Lau B, Wong S, Isserow BS. The science of exercise prescription: Martti Karvonen and his contributions. BCMJ. 2017; 59:38-41.
14. 14. Sun Y, Oberley LW, Li YA. A simple method for clinical assay of superoxide dismutase, Clin Chem. 1988;34:497-500.
15. 15. Yasmineh WG, Kaur TP, Blazar BR, Theologides A. Serum catalase as marker of graft-vs-host disease in allogeneic bone marrow transplant recipients: pilot study. Clin Chem. 1995; 41:1574-80.
16. 16. Paglia, DE, Valentina WN. Studies on quantitative and qualitative characterization of erytrocte glutathione peroxidase. Journal of Laboratory and Clin Med. 1967;70: 158-69.
17. 17. Eckerson HW, Romson J, Wyte C, La Du BN. The human serum paraoxonase polymorphism: identification of phenotypes by their response to salts. Am J Hum Genet. 1983;35:214-27.
18. 18. Evans P, Lyras L, Halliwell B. Measurement of Potein carbonyls in human brain tissue. Metods Enzymol. 1999; 300:145-156.
19. 19. Yoshoiko T, Kawada K, Shimada T. Lipid peroxidation in maternal and cord blood and protective mechanism against actived-oxygen toxicity in the blood. AJOG. 1979;135:372-6.
20. 20. Smárason AK, Allman KG, Young D, Redman CW. Elevated levels of serum nitrate, a stable end product of nitric oxide, in women with pre-eclampsi, BJOG. 1997;104:538-43.
21. 21. Rowicka G, Dylag H, Ambroszkiewicz J, Riahi A, Weker H, Chełchowska M. Total Oxidant and Antioxidant Status in Prepubertal Children with Obesity. Oxid Med Cell Longev. 2017;2017:5621989.
22. 22. Kilic E, Özer ÖF, Erek Toprak A, et al. Oxidative Stress Status in Childhood Obesity: A Potential Risk Predictor. Med Sci Monit. 2016;22: 3673-9. 23. Phillips MD, Patrizi RM, Cheek DJ, Wooten JS, Barbee JJ, Mitchell JB. Resistance training reduces subclinical inflammation in obese, postmenopausal women. Med Sci Sports Exerc. 2012; 44:2099-110.
23. 24. Vincent HK, Bourguignon C, Vincent KR. Resistance training lowers exercise-induced oxidative stress and homocysteine levels in overweight and obese older adults. Obesity (Silver Spring). 2006;14:1921-30.
24. 25. Oh S, Tanaka K, Warabi E, Shoda J. Exercise reduces inflammation and oxidative stress in obesity-related liver diseases. Med Sci Sports Exerc. 2013;45:2214-22.
25. 26. Samjoo IA, Safdar A, Hamadeh MJ, Raha S, Tarnopolsky MA. The effect of endurance exercise on both skeletal muscle and systemic oxidative stress in previously sedentary obese men. Nutr Diabetes. 2013;16;3:88.
26. 27. Radák Z, Apor P, Pucsok J, et al. Aerobic training suppresses exercise-induced lipid peroxidation and inflammation in overweight/obese adolescent girls. Pediatr Exerc Sci. 2015;27:67–76.
27. 28. Kelly AS, Steinberger J, Olson TP, Dengel DR. In the absence of weight loss, exercise training does not improve adipokines or oxidative stress in overweight children. Metabolism. 2007; 56:1005-9.
28. 29. Powers SK, Jackson MJ. Exercise-induced oxidative stress: cellular mechanisms and impact on muscle force production. Physiol Rev. 2008;88:1243-76.
29. 30. Huang CJ, McAllister MJ, Slusher AL, Webb HE, Mock JT, Acevedo EO. Obesity-Related Oxidative Stress: the Impact of Physical Activity and Diet Manipulation. Sports Med Open. 2015;1:32.