ANALYSIS OF THIOLE/DISULFIDE HOMEOSTASIS IN PATIENTS WITH DECUBITUS ULCER

Yıl 2022, Cilt: 3 Sayı: 2, 345 - 353, 22.08.2022

Hamit Yıldız Deniz Yıldız Pehlivan

Öz

Aim: There is a growing body of literature that recognises the importance of decubitus ulcer. Decubitus ulcer is tissue necrosis and ulceration that inevitably occurs in tissues exposed to pressure for a long time. Reoxygenation of ischemic tissue results in the formation of reactive oxygen metabolites, during ischemia/reperfusion. Thiols are antioxidant substances belonging to the mercaptan group. This study aims to contribute to this growing area of research by exploring thiol/disulfide levels, a new oxidative stress parameter, in patients with decubitus ulcer.
Materials and Method: This study consists of two groups, one is the control group and the other is the patient group. While the control group consists of 50 healthy individuals, the patient group consists of 50 patients with decubitus ulcer. Native thiol and total thiol levels were measured by spectrophotometric method. Data management and analysis were performed using SPSS software (version 22).
Results: While the average native thiol, total thiol level, reduced thiol ratio, and thiol oxidation reduction ratio levels in the patient group were lower than the control group; disulfide and oxidized thiol levels were higher than the control group
Conclusion: Total thiols, native (free) thiols and disulfide levels were significantly changed in the patient group compared to healthy individuals. The most striking result to emerge from the data is that plasma dynamic thiol/disulfide levels alter and shift to the disufide side in patients with decubitus ulcers.

Anahtar Kelimeler

Thiol/disulfide homeostasis, decubitus ulcer, oxidative stress, tissue damage

Kaynakça

• 1. Liao X, Ju Y, Liu G, Zhao X, Wang Y, Wang Y. Risk factors for pressure sores in hospitalized acute ischemic stroke patients. Journal of stroke and cerebrovascular diseases. 2019;28(7):2026–30.
• 2. Kumar N, Rao S. Ayurvedic management of dusta vrana w.s.r. decubitus ulcer: a case study. Journal of ayurveda and integrated medical sciences. 2020;5(4):434–7.
• 3. Yarkony GM. Pressure ulcers: a review. Arch phys bled rehabil. 1994;75:908–17.
• 4. Salcido R, Popescu A, Ahn C. Animal models in pressure ulcer research. J spinal cord med. 2007;30:107–16.
• 5. Halliwell B. Reactive oxygen species in living systems: source, biochemistry, and role in human disease. The american journal of medicine. 1991;91:14–22. 6. Latha B, Babu M. The involvement of free radicals in burn injury: a review. Burns. 2001;27:309–17.
• 7. Sen CK, Packer L. Thiol homeostasis and supplements in physical exercise. American journal of clinical nutrition. 2000;72:653–69.
• 8. Tanrıverdi F, Yüzbasioglu Y, Ercan Haydar FG, Gökhan S, Özhasenekler A, Yıldırım Ç, et al. Evaluation of thiol disulphide homeostasis and neutrophile lymphocyte ratio in carbon monoxide intoxication. Ankara medical journal. 2020;20(1):47–56.
• 9. Elmas B, Karacan M, Dervişoğlu P, Kösecik M, İşgüven ŞP, Bal C. Dynamic thiol/disulphide homeostasis as a novel indicator of oxidative stress in obese children and its relationship with inflammatory-cardiovascular markers. Anatolian journal of cardiology. 2017;18:361–9.
• 10. Elmas B, Yıldız T, Yazar H, İlçe Z, Bal C, Özbek B, et al. New oxidative stress markers useful in the diagnosis of acute appendicitis in children: Thiol/disulfide homeostasis and the asymmetric dimethylarginine level. Pediatric emergency care. 2020;36(8):362–7.
• 11. Yıldız H. Thiol/disulphide homeostasis in intensive care unit patients with sepsis and septic shock. Turkish journal of medical sciences. 2020;50:811–6. 12. Erel O, Neselioglu S. A novel and automated assay for thiol/disulphide homeostasis. Clinical biochemistry. 2014;47(18):326–32.
• 13. Leimkuhler M, Bourgonje A, van Goor H, van Leeuwen B, Bock de G. Systemic oxidative stress and antioxidant capacity in cancer patients. Journal of translational science. 2020;6:1–3.
• 14. Şener G, Sert G, Şehirli AÖ, Arbak S, Uslu B, Gedik N, et al. Pressure ulcer-induced oxidative organ injury is ameliorated by B-glucan treatment in rats. International immunopharmacology. 2006;6:724–32.
• 15. Kumar S, Theis T, Tschang M, Nagaraj V, Berthiaume F. Reactive oxygen species and pressure ulcer formation after traumatic injury to spinal cord and brain. Antioxidants. 2021;10:1–15.
• 16. Şener G, Sert G, Şehirli AÖ, Arbak S, Gedik N, Ayanoğlu-Dülger G. Melatonin protects against pressure ulcer-induced oxidative injury of the skin and remote organs in rats. J pineal res. 2006;40:280–7.
• 17. Castro L, Freeman BA. Reactive oxygen species in human health and disease. Nutrition. 2001;17:161–5.
• 18. Khlifi L, Graiet H, Sahli S, Ben-hadj-mohamed M, Bouzidi N, Miled AE. Evidence of metabolic imbalance and oxidative stress among patients suffering from pressure ulcers. Journal of dermatologıcal treatment. 2019;30(4):414–21.
• 19. Apaydın ZP, Yıldız H, Samim FS, Alaşehirli B. Investigation of dynamic thiol disulfide homeostasis in acute respiratory failure patients in intensive care unit. Experimental and applied medical science. 2021;2(2):164–75.