Investigation of the Effect of Toluene on Nitric Oxide Production and Protective Properties of Resveratrol

Yıl 2023, Cilt: 2 Sayı: Kongre Özel Sayısı - 3. Uluslararası Multidisipliner Kanser Araştırmaları Kongresi, 91 - 101, 31.10.2023

Egemen Dere , Raissa Soamanjary

https://doi.org/10.59312/ebshealth.1362893

Öz

Aim: Toluene is the most preferred organic solvent. Prolonged exposure to toluene, causes serious health problems. The International Agency for Research on Cancer (IARC) has classified toluene as "possibly carcinogenic to humans" (Group 2B). This classification states that toluene may have potential carcinogenic effects. Toluene exposure has been linked to the formation of reactive nitrogen species and reactive oxygen species, resulting in direct tissue damage and alteration of various antioxidant systems. Resveratrol, which has a polyphenol structure, is a molecule that has important effects on plants. It is known to have positive effects on humans as well. In this study, the effect of exposure to toluene on nitric oxide production, which has an important role as a biological regulator in cardiovascular, neurological, immunological and many other systems, and the protective properties of resveratrol were investigated.

Materials and Methods While 900mg/kg dose of toluene was administered intraperitoneally to male rats (250-350gr Wistar-Albino), 5, 10 and 20mg/kg doses of resveratrol were administered the same way for six days. Nitric oxide levels and Nitric oxide synthase activities were investigated in liver tissue and serum.

Finding: The data showed an increased nitric oxide level in the liver tissue and serum and a high nitric oxide synthase activity following toluene administration. Significant reductions in nitric oxide levels and nitric oxide synthase activity in the liver were observed after the administration of various dosages of resveratrol.

Results: Our results suggested that high doses of toluene induce nitric oxide production, whereas resveratrol possesses protective properties.

Anahtar Kelimeler

Toluene, resveratrol, nitric oxide, nitric oxide synthase

Toluenin Nitrik Oksit Üretimine Etkisinin ve Resveratrolün Koruyucu Özelliklerinin Araştırılması

Öz

Amaç: Organik çözücü olarak en çok tercih edilen Toluendir. Toluene uzun süre maruz kalmak ciddi sağlık sorunlarına neden olur. Uluslararası Kanser Araştırmaları Birliği (IACR), tolüeni "insanlar için muhtemelen kanserojen" (Grup 2B) olarak sınıflandırmıştır. Bu sınıflandırma, toluenin potansiyel kanserojen etkilere sahip olabileceğini belirtir. Toluene maruz kalma, reaktif nitrojen türlerinin ve reaktif oksijen türlerinin oluşumuyla bağlantılı olup, doğrudan doku hasarına ve çeşitli antioksidan sistemlerin değişmesine neden olur. Polifenol yapısında olan resveratrol, bitkilerde önemli etkilere sahip bir moleküldür. İnsanlarda da olumlu etkilere sahip olduğu bilinmektedir. Bu çalışmada, kardiyovasküler, nörolojik, immünolojik ve diğer birçok sistemde biyolojik düzenleyici olarak önemli bir role sahip olan nitrik oksit üretimine toluene maruz kalmanın etkisi ve resveratrolün koruyucu özellikleri araştırılmıştır.

Yöntem: Tolüenin 900mg/kg dozu erkek sıçanlara (250-350gr Wistar-Albino) intraperitoneal yolla uygulanırken resveratrol 5, 10 ve 20mg/kg dozları aynı yolla altı gün boyunca uygulanmıştır. Karaciğer dokusunda ve serumda nitrik oksit seviyeleri ve nitrik oksit sentaz aktiviteleri araştırılmıştır.

Bulgular: Veriler, karaciğer dokusunda ve serumda nitrik oksit seviyesinin arttığını ve toluen uygulamasını takiben yüksek nitrik oksit sentaz aktivitesini gösterdi. Resveratrolün çeşitli dozlarının uygulanmasından sonra karaciğerde nitrik oksit seviyelerinde ve nitrik oksit sentaz aktivitesinde önemli azalmalar gözlendi.

Sonuçlar: Sonuçlarımız yüksek dozda toluenin nitrik oksit üretimini tetiklediğini, resveratrolün ise koruyucu özelliklere sahip olduğunu gösterdi.

Anahtar Kelimeler

Toluene, resveratrol, nitric oxide, nitric oxide synthase

Etik Beyan

Ethics Committee Approval: This study was conducted with the ethics committee decision (2019-04/03) of Bursa Uludağ University’s local ethics committee for animal experiments

Destekleyen Kurum

This work was funded by Bursa Uludağ University Scientific Research Projects Unit (project number QUAP(F)-2019/14).

Teşekkür

We thank Emine Gonca PEKEL, Oğuzhan Akgün and Ahmad Baratov for their valuable contributions to this study.

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