MIR-145'in Meme Kanserinin İlerlemesinde Düzenleyici Rolü

Yıl 2023, Cilt: 2 Sayı: 3, 110 - 117, 30.11.2023

Boran Can Dinçer

https://doi.org/10.59312/ebshealth.1367068

Öz

Meme kanseri tanısı geç konulduğunda tedavi ve sağkalım oranları azalmaktadır. Her yıl milyonlarca kadın meme kanseri teşhisi almakta ve bu hastalık nedeniyle birçok insan hayatını kaybetmektedir. Ancak, erken teşhisi sağlayacak biyobelirteçlerin keşfi ile tedavi süreçlerinin daha başarılı olacağı ve sağkalım sürelerinin arttırılması hedeflenmektedir. Son yıllarda, mikroRNA'ların biyobelirteç olarak meme kanseri erken teşhisinde kullanımı ile ilgili çalışmalar yapılmaktadır. Araştırmalar, mikroRNA'ların meme kanseri teşhisi ve prognoz tahmini için kullanılabilecek biyobelirteçler olabileceğini göstermektedir. mikroRNA'ların meme kanseri erken tanısında biyobelirteç olarak potansiyel kullanımı üzerine devam eden araştırmalarla birlikte, çalışmamızda da miR-145'in meme kanserinde biyobelirteç olarak kullanılabilme potansiyeli analiz edildi. Çalışmamızda, 200 meme kanseri hastasının ve sağlıklı kontrol grubunda yer alan 100 kişinin kan-serum numunelerinden RNA örnekleri izole edilerek, mir-145 özgün ekspresyon seviyeleri RT-qPCR tekniği ile tespit edildi. Bu analizler sonucunda, miR-145'in meme kanseri hastalarında sağlıklı kontrol grubuna göre daha düşük düzeylerde ekspresyon seviyeleri bulundu. miR-145'in biyobelirteç olabilme niteliğinin daha yüksek sayılı meme kanseri hasta kohortunda araştırıldıktan sonra meme kanseri erken teşhisinde hedef biyobelirteç olarak kullanılabileceği hedeflenmektedir.

Anahtar Kelimeler

Meme kanseri, mikroRNA, miR-145

MIR-145's Regulatory Role in Breast Cancer Progression

Öz

Delayed diagnosis of breast cancer results in lower treatment success rates and lower survival rates. Every year millions of women are diagnosed with breast cancer and many of them die from this disease. However, the discovery of biomarkers that enable early diagnosis should increase the success of treatment processes and extend survival times. In recent years, studies have been carried out to use microRNAs as biomarkers in the early detection of breast cancer. Research shows that microRNAs can serve as potential biomarkers for the diagnosis and prognosis of breast cancer. In addition to ongoing research on the possible use of microRNAs as biomarkers for early breast cancer diagnosis, our study also analyzed the possible use of miR-145 as a biomarker in breast cancer. In our study, RNA samples were isolated from blood serum samples of 200 breast cancer patients and 100 people in the healthy control group, and the specific expression levels of miR-145 were determined using RT-qPCR technique. As a result of these analyses, it was found that miR-145 had lower expression levels in breast cancer patients than in healthy controls. After further investigation of the biomarker potential of miR-145 in a larger group of breast cancer patients, it will be used as a target biomarker for early breast cancer diagnosis.

Anahtar Kelimeler

Breast cancer, microRNA, miR-145

Kaynakça

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