Farmasötik İlaçta İzoprenalin Tayini İçin Potansiyometrik Sensör Geliştirilmesi

Yıl 2020, Cilt: 7 Sayı: 1, 211 - 222, 31.01.2020

Gülşah Saydan Kanberoğlu Oktay Özarslan

https://doi.org/10.31202/ecjse.616663

Öz

Bu
çalışmada, farmasötik etken madde olan izoprenalinin tayini için potansiyometrik
iyon seçici bir elektrot (İSE) geliştirildi. Bu amaçla, ilk olarak, İP-FM,
İP-TSA, İP-TPB, İP-REY, İP-FTA iyon çiftleri sentezlendi. Sentezlenen iyon
çiftleri elektrot membranının yapısında iyonofor olarak kullanıldı. Membran
optimizasyonunu sağlamak için sentezlenen iyon çiftleri kullanılarak çeşitli
bileşimlerde PVC membran elektrotlar üretildi. Bu elektrotların potansiyometrik
performans özellikleri araştırıldı. En iyi potansiyometrik performans
özelliklerinin % 3.0 İzoprenalin-Tetrafenilborat iyon çifti, % 64
Nitrofeniloktileter (NPOE), % 32.0 Polivinilklorür (PVC), % 1 Potasyum tetrakis
(4-klorofenil) borat (KTpClPB) bileşimine sahip membran kullanılarak elde
edildi. Bu elektrodun doğrusal çalışma aralığının 5.0x 10^-6 M-1.0x
10^-1 M ve 10 katlık konsantrasyon değişimindeki eğimi, 45,3 mV;
tayin limiti, 5.0x10^-6 M; pH çalışma aralığı 2.6-3.6 ve 5.7-7.9;
cevap zamanı < 5 sn olarak belirlendi. Elektrot oldukça tekrarlanabilir bir
potansiyometrik cevap sergilemiştir. İzoprenalin içeriği, izoprenalin seçici elektrot
kullanılarak ilaçta tayin edildi.  

Anahtar Kelimeler

İzoprenalin, Potansiyometrik sensör

Destekleyen Kurum

van yüzüncü yıl üniversitesi bilimsel araştırma projesi başkanlığı

Proje Numarası

FYL-2018-7184

Development of Potentiometric Sensor for Determination of Isoprenaline in Pharmaceutical Drug

Öz

In this study, a potentiometric
ion-selective electrode (ISE) was developed for the determination of isoprenaline,
a pharmaceutical active substance. For this purpose, initially, IP-PM, IP-TCA,
IP-TPB, IP-REY, IP-PTA ion pairs were synthesized. The synthesized ion pairs
were used as the ionophore in the structure of the electrode membrane. In order
to ensure membrane optimization, PVC membrane ion selective electrodes were
produced in various compositions using the synthesized ion pair and the
potentiometric performance characteristics of these electrodes were
investigated. It was determined that the best potentiometric performance
characteristics were obtained with the PVC membrane electrode in the composition
of 3.0% isoprenaline-tetrafenylborate ion pair, %64.0 nitrophenyloctylether,
32.0% polyvinylchloride and %1.0 Tetrakis (4-chlorophenyl) boron potassium
compound. The linear operating range of this electrode is 5.0x 10^-6
M - 1.0x 10^-1 M and the slope at the 10-fold concentration change is
45.3 mV; determination limit, 5.0x10^-6 M, pH working range, 2.6-3.6
and 5.7-7.9; response time < 5s. The electrode exhibited a reproducible
potentiometric response. Isoprenaline content was determined in drug by using
the isoprenaline selective electrode. 

Anahtar Kelimeler

Isoprenaline, potentiometric sensor

Proje Numarası

FYL-2018-7184

Kaynakça

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