Cytotoxic Activity of Carrageenan on Malignant MCF-7 Breast Cancer and the non-Malignant SVCT Breast Epithelial Cell Lines

Öz

Objective: Recent studies have shown that sulfated polysaccharides obtained from marine algae have many biological and physiological activities. Kappa-carrageenan, a sulfated polysaccharide obtained from red marine algae, is known to have anti-proliferative effects in various cancer cell lines. In this study, the cytotoxic effect of kappa-carrageenan on two different cell lines, namely the malignant MCF-7 breast cancer cell line and the non-malignant SVCT breast epithelial cell lines, was investigated in an in vitro model.

Materials & Methods: Three different concentrations of carrageenan were prepared for each cell in its own medium, with an initial dose of 1000 µg/ml (Dilution I: 1000 µg/ml; Dilution II: 250 µg/ml; Dilution III: 62.5 µg/ml). Cells were incubated with carrageenan at the doses set for each experimental group. Cells incubated in a carrageenan-free medium comprised the control group.

Results: To measure cell viability, 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) analysis was performed. Cell morphology was investigated by the acridine orange (AO)/propidium iodide (PI) fluorescent staining method. The present results indicated that carrageenan caused cytotoxic effects on both malignant and non-malignant cell lines.

Conclusion: Considering that the different phenotypic features of the subtypes of the MCF-7 cell line may affect cell viability and cell proliferation, cell selection should be performed very carefully in cytotoxicity studies. We suggest that using the MCF-7 cell line for cytotoxicity experiments needs to contemplate this important phenomenon for further experimental setups.

Anahtar Kelimeler

• Carrageenan
• cytotoxicity
• MCF-7
• SVCT
• MTT

Karragenanın Malign MCF-7 Meme Kanseri ve Malign Olmayan SVCT Meme Epitel Hücre Dizileri Üzerindeki Sitotoksik Aktivitesi

Öz

Amaç: Son yapılan çalışmalar deniz alglerinden elde edilen sülfatlanmış polisakkaritlerin birçok biyolojik ve fizyolojik aktivitesi olduğunu göstermektedir. Kırmızı deniz alglerinden elde edilen sülfatlanmış bir polisakkarit olan kappa-karragenanın da çeşitli kanser hücre hatlarında anti-proliferatif etki gösterdiği bilinmektedir. Bu çalışmada kappa-karragenanın, iki farklı hücre hattı, malignant özellikte MCF-7 meme kanseri hücre hattı ve non-malignant SVCT meme epiteli hücre hatları üzerindeki sitotoksik etkisi in vitro modelde incelenmiştir.

Gereç ve Yöntem: Karragenanın 1000 µg/ml başlangıç dozu olacak şekilde ve her hücre için kendi besiyerinde üç farklı konsantrasyonu hazırlanmıştır (Dilution I: 1000 µg/ml; Dilution II: 250 µg/ml; Dilution III: 62,5 µg/ml). Hücreler deney gruplarında belirlenen dozlarda karregenan ile inkübe edilmiştir. Karragenan içermeyen besiyeinde inkübe edilen hücreler de kontrol grubu olarak alınmıştır.

Bulgular: Hücre canlılığının ölçülmesi için, (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) (MTT) analizi yapılmıştır. Hücre morfolojisi, acridine orange (AO)/propidium iodide (PI) floresan boyama yöntemi ile incelenmiştir. Çalışmamızda elde ettiğimiz sonuçlar, karragenanın hem malignant hem de non-malignant hücre hatları üzerinde sitotoksik etkiye neden olduğunu göstermektedir.

Sonuç: MCF-7 hücre hattının alt tiplerinin gösterdiği farklı fenotipik özelliklerin hücre canlılığı ve hücre proliferasyonunu etkileyebildiği de göz önünde bulundurularak sitotoksite çalışmalarında hücre seçiminin çok dikkatli yapılması ve MCF-7 hücre hattının kullanılması halinde bu durumun önemle üzerinde durulması gerektiği düşünülmektedir.

Anahtar Kelimeler

• Karragenan
• sitotoksite
• MCF-7
• SVCT
• MTT

Kaynakça

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