The effects of Hypericum crenulatum polysaccharides on the proliferation of cancer cells

İşil Aydemir; Oktay Ozkan; Ahmet Savran

doi:10.33713/egetbd.647464

The effects of Hypericum crenulatum polysaccharides on the proliferation of cancer cells

Öz

The goal in the cancer treatment is to induce tumor cell death without damaging healthy cells and to prevent the spread of these cells to other organs. In the case of metastasis, the cure protocols may be inadequate. Alternative methods are used to support treatment. For this purpose, herbal products are widely used. In our experiment, we aimed to investigate the effect of Hypericum crenulatum polysaccharides on the proliferation of breast cancer cell lines. Hypericum crenulatum polysaccharides were isolated and were exposed to the MCF-7 and MDA-MB-231 cells at different dilutions, then IC₅₀ doses for each cells were calculated using MTT assay. Breast cancer cells were treated with IC₅₀ doses of polysaccharides for 24 hours and immunocytochemistry was performed to evaluate the expressions of metastases and proliferation markers, PI3K, Akt-1 and Erk-1/2. The staining results were evaluated by H-score and the difference between the groups was analyzed statistically. Hypericum crenulatum polysaccharides were found to be toxic to both breast cancer cells by MTT method. When PI3K, Akt-1 and Erk-1/2 markers of proliferation and metastasis were evaluated, it was seen that there was a significant decrease compared to the control group. Since MDA-MB-231 cells are ER (-), their invasion ability is over compared to MCF-7 ER (+) cells. For this reason, the toxic effect of polysaccharides was found to be higher in MCF-7 cells. As a result, in vivo studies are needed to be performed following the the antiproliferative effects of herbal products are found in vitro.

Anahtar Kelimeler

Hypericum crenulatum,breast cancer

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Klinik Tıp Bilimleri

Bölüm

Araştırma Makalesi

Yazarlar

İşil Aydemir ^*
0000-0002-4143-7319
Türkiye

Oktay Ozkan Bu kişi benim
0000-0002-9794-8863
Türkiye

Ahmet Savran Bu kişi benim
0000-0003-4609-9547
Türkiye

Yayımlanma Tarihi

21 Nisan 2020

Gönderilme Tarihi

15 Kasım 2019

Kabul Tarihi

25 Aralık 2019

Yayımlandığı Sayı

Yıl 2020 Cilt: 3 Sayı: 1

DOI

https://doi.org/10.33713/egetbd.647464

IZ

https://izlik.org/JA54DE29UA

Kaynak Göster

RIS / Bibtex

APA

Aydemir, İ., Ozkan, O., & Savran, A. (2020). The effects of Hypericum crenulatum polysaccharides on the proliferation of cancer cells. Ege Tıp Bilimleri Dergisi, 3(1), 1-6. https://doi.org/10.33713/egetbd.647464

AMA

1.Aydemir İ, Ozkan O, Savran A. The effects of Hypericum crenulatum polysaccharides on the proliferation of cancer cells. AEGEAN J MED SCI. 2020;3(1):1-6. doi:10.33713/egetbd.647464

Chicago

Aydemir, İşil, Oktay Ozkan, ve Ahmet Savran. 2020. “The effects of Hypericum crenulatum polysaccharides on the proliferation of cancer cells”. Ege Tıp Bilimleri Dergisi 3 (1): 1-6. https://doi.org/10.33713/egetbd.647464.

EndNote

Aydemir İ, Ozkan O, Savran A (01 Nisan 2020) The effects of Hypericum crenulatum polysaccharides on the proliferation of cancer cells. Ege Tıp Bilimleri Dergisi 3 1 1–6.

IEEE

[1]İ. Aydemir, O. Ozkan, ve A. Savran, “The effects of Hypericum crenulatum polysaccharides on the proliferation of cancer cells”, AEGEAN J MED SCI, c. 3, sy 1, ss. 1–6, Nis. 2020, doi: 10.33713/egetbd.647464.

ISNAD

Aydemir, İşil - Ozkan, Oktay - Savran, Ahmet. “The effects of Hypericum crenulatum polysaccharides on the proliferation of cancer cells”. Ege Tıp Bilimleri Dergisi 3/1 (01 Nisan 2020): 1-6. https://doi.org/10.33713/egetbd.647464.

JAMA

1.Aydemir İ, Ozkan O, Savran A. The effects of Hypericum crenulatum polysaccharides on the proliferation of cancer cells. AEGEAN J MED SCI. 2020;3:1–6.

MLA

Aydemir, İşil, vd. “The effects of Hypericum crenulatum polysaccharides on the proliferation of cancer cells”. Ege Tıp Bilimleri Dergisi, c. 3, sy 1, Nisan 2020, ss. 1-6, doi:10.33713/egetbd.647464.

Vancouver

1.İşil Aydemir, Oktay Ozkan, Ahmet Savran. The effects of Hypericum crenulatum polysaccharides on the proliferation of cancer cells. AEGEAN J MED SCI. 01 Nisan 2020;3(1):1-6. doi:10.33713/egetbd.647464

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Anti-cancer Potential of Hypericum spp. with Focus on Hypericum Perforatum: A Review of the Literature

Current Traditional Medicine

https://doi.org/10.2174/2215083808666220822141221