Use of Artemisia campestris (L) on Treatment of Spironucleosis Infection in Rainbow Trout (Oncorhynchus mykiss)

Abstract

The aim of the present study was to determine the in vivo antiparasitic activity of the Artemisia campestris (L) ethanol extracts for control of Spironucleosis (Hexamitiasis) in rainbow trout (Oncorhynchus mykiss). Treated rainbow trout with an average weight of 1.5-2.0 g, were fed with 7 and 21-day periods with the addition of plant extracts of 1.0, 1.5, 2.0, 2.5 and 3.0 g / kg. The composition of ethanol extract of A. campestris (L) was analyzed by GC-MS and the major components were acenaphthylene 1,2–dihydro (62.70%), n-octadecane (4.66%), capillin (4.95%), curcumene (4.45%), 2.4-pentadiynylbenzene (7.28%), benzaldehyde (2.53%), methacrolein (1.35%). The results of the present study showed that cumulative mortalities were decreased in all treatment groups and positive control, compared to the negative control (p<0.05). It was determined that 21-day treatment was more effective than 7-day treatment on the intensity and prevalence of infection. These results suggested that the ethanol extract of A. campestris (L) displayed the antiparasitic activity against S. salmonis.

Keywords

• Artemisia campestris L.,Hexamita salmonis,rainbow trout (Oncorhynchus mykiss),antiparasitic,treatment

Gökkuşağı Alabalığı (Oncorhynchus mykiss) Yetiştiriciliğinde Görülen Spironucleosis Enfeksiyonlarının Tedavisinde Artemisia campestris (L)’in Kullanımı

Öz

Bu çalışmada, Artemisia campestris (L) bitkisi etanol ekstraktının yavru gökkuşağı alabalıklarında görülen Spironucleosis (Hexamitiasis) enfeksiyonları üzerine in vivo antiparazitik aktivitesinin belirlenmesi amaçlanmıştır. Ortalama ağırlıkları 1,5-2,0 g olan enfekte gökkuşağı alabalıklarına, 1,0, 1,5, 2,0 2,5 ve 3,0 g/kg oranlarında bitki ekstraktı ilaveli yemlerle 7 gün ve 21 gün periyotlarda tedavi uygulaması yapılmıştır. A. campestris (L) etanol ekstraktının GC-MS ile analizlenmesi sonucunda major bileşenleri; acenaphthylene 1,2– dihydro (%62,70), n-octadecane (%4,66), capillin (%4,95), curcumene (%4,45), 2.4-pentadiynylbenzene (%7,28), benzaldehyde (%2,53), methacrolein (%1,35) olarak tespit edilmiştir. Tedavi uygulaması sonunda elde edilen sonuçlara göre, deney gruplarında ve pozitif kontrol grubunda kümülatif mortalitenin negatif kontrol grubuna göre azaldığı görülmüştür (p<0,05). Enfeksiyonun yoğunluğu ve oranı üzerine 21 günlük tedavi uygulamasının 7 günlük uygulamadan daha etkili olduğu belirlenmiştir. Araştırma sonuçlarımızda, A. campestris (L) ekstraktının gökkuşağı alabalıklarında Spironucleus salmonis’e karşı antiparazitik etki sağladığı belirlenmiştir. Anahtar kelimeler Artemisia campestris(L), Spironucleus salmonis, Oncorhynchus mykiss, antiparaziter, tedavi. 

Anahtar Kelimeler

• Artemisia campestris L.,Hexamita salmonis,gökkuşağı alabalığı (Oncorhynchus mykiss),antiparaziter,tedavi

Kaynakça

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