İnvestigation of the Efficacy of Aminoguanidine in an Experimental Rat Model with Isolated Bilateral Pulmonary Contusion Due To Blunt Thoracic Trauma

Öz

İntroduction: In severe thoracic trauma pulmonary contusions are almost inevitable are associated with high morbidity and mortality. In this study we aimed to evaluate the antioxidant activity of aminoguanidine in pulmonary contusion.

Method: Sixty-three Sprague-male rats were used. Sham and aminoguanidine groups were exposed to isolated blunt thoracic trauma with a force of 1,512 joules. Aminoguanidine was administered intraperitoneally at a dose of 100 mg/kg 3 hours before the trauma and on the 1. and 2. day after the trauma. The contusion group was exposed to blunt thoracic trauma only. In all groups, arterial blood gas analysis and catalase and NO levels were done on the 0th, 1st, 2nd and 3rd days.

Results: PO2 levels were higher in the sham group compared to the contusion group, without statistical significance. On the third day, SaO2 levels were higher in the AG group compared to the contusion group. SaO2 levels were comparable in the AG and sham groups on days 1, 2 and 3. There was no difference between the PaO2 levels of the contusion and sham groups on the 2nd and 3rd days. There was no difference between the PaO2 levels of the AG and sham groups on the 1st, 2nd and 3rd days. We found no difference between the PaCO2 levels of the contusion and sham groups on the 0-3 days. There was no difference between the PaCO2 levels of the AG and sham groups on the 1st, 2nd and 3rd days. No difference was observed between the PaCO2 levels of the AG and contusion groups on the 1st, 2nd and 3rd days. No significant difference was found between the NO levels of the sham and the contusion groups on day 0. There was a significant difference between the sham and contusion groups on the 1st, 2nd and 3rd days. There was no statistically significant difference between the catalase enzyme activities of the sham and AG groups.

Conclusion: In our study, we showed that the use of aminoguanidine did not significantly reduce the severity of pulmonary contusion and the inflammatory reaction induced by thoracic trauma in rats.

Anahtar Kelimeler

• Blunt Thoracic Trauma,Aminoguanidine,Catalase activity

Kaynakça

1. 1. Byun CS, Park IH, Oh JH, Bae KS, Lee KH, Lee E. Epidemiology of trauma patients and analysis of 268 mortality cases: trends of a single center in Korea. Yonsei Med J 2015; 56: 220– 6.
2. 2. Pehlivanlar Küçük M, Küçük AO, Aksoy İ, Aydın D, Ülger F. Prognostic evaluation of cases with thoracic trauma admitted to the intensive care unit: 10-year clinical outcomes. Ulus Travma Acil Cerrahi Derg 2019; 25(1): 46- 54.
3. 3. Alisha C, Gajanan G, Jyothi H. Risk Factors Affecting the Prognosis in Patients with Pulmonary Contusion Following Chest Trauma. J Clin Diagn Res 2015; 9(8): 17- 9.
4. 4. Rendeki S, Molnar. Pulmonary contusion. TFJ Thorac Dis 2019 ; 11:141- 151.5. Rus A, Peinado MA, Castro L, Del Moral ML. Lung eNOS and iNOS are reoxygenation time-dependent upregulated after acute hypoxia. Anat Rec (Hoboken) 2010; 293(6): 1089- 98.
5. 6. Kubes P, McCafferty DM. Nitric oxide and intestinal inflammation. Am J Med 2000; 109: 150- 58.
6. 7. Nieves C Jr, Langkamp-Henken B. Arginine and immunity: a uniqueper spective. Biomed Pharmacother 2002; 56: 471- 82
7. 8. Topcu K, Kırıcı DÖ, Evcil MS. Catalase activity in healthy and inflamed pulp tissues of permanent teeth in young people. Niger J Clin Pract 2016; 19(5): 600- 29. Tovar J.A., Vazquez J.J. Management of chest trauma in children. Paediatr. Respir. Rev 2013; 14: 86– 91.
8. 10. Tignanelli CJ, Hemmila MR, Rogers MAM, Raghavendran K. Nationwide cohort study of independent risk factors for acute respiratory distress syndrome after trauma. Trauma Surg Acute Care Open 2019; 15; 4(1): 249.

9. 11. Durham RM, Moran JJ, Mazuski JE, Shapiro MJ, Baue AE, Flint LM. Multiple organ failure in trauma patients J Trauma 2003; 55: 608– 1612. Akkuş I, Gültekin F, Aköz M, Çağlayan O, Bahçeci S, Can UG, Ay M, Gürel A. Effect of moderate alcohol intake on lipit peroxidation in plasma, erythrocyte and leukocyte and on some antioxidant enzymes. Clin Chim Acta 1997; 31: 266, 141-47
10. 13. Bayir H, Marion DW, Puccio AM, Wisniewski SR, Janesko KL, Clark RS, Kochanek PM. Marked gender effect on lipid peroxidation after severe traumatic brain injury in adult patients. J Neurotrauma. 2004; 21: 1– 8.
11. 14. Scholpp J, Schubert JK, Miekisch W, Noeldge-Schomburg GF. Lipid peroxidation early after brain injury. J Neurotrauma. 2004; 21(6): 667- 77. 15. Al Drees A, Salah Khalil M, Soliman M. Histological and Immunohistochemical Basis of the Effect of Aminoguanidine on Renal Changes Associated with Hemorrhagic Shock in a Rat Model. Acta Histochem Cytochem. 2017; 28; 50(1): 11- 19.
12. 16. Giri SN, Biring I, Nguyen T, et al. Abrogation of bleomycin-induced lung fibrosis by nitric oxide synthase inhibitor, aminoguanidine in mice. Nitric Oxide 2002; 7(2): 109- 18.
13. 17. Jang AS, Choi IS, Lee S, et al. Nitric oxide metabolites in induced sputum; a marker of airway inflammation in asthmatic subjects. Clin Exp Allergy 1999; 29: 1139- 42.
14. 18. Krishnan R., Bruce A., Jadwiga D., Cristi J., Patricia M., James A., Paul R. A rat model of ısolated bilateral lung contusion from blunt chest trauma. Anest analg. 2005; 1001: 1482- 89