In vitro study on antioxidant, antibacterial and DNA interaction activities of extracts from Arbutus andrachne L.

Abstract

Traditionally, the fruits and leaves of Arbutus andrachne are well known and used in for applications in antimicrobial, antioxidant and anticancer activity. This study was aimed to determine the in vitro antibacterial, antioxidant and DNA interaction activities of the different extracts of Arbutus andrachne.

Arbutus andrachne was collected from Amasya Province during May 2018. Dried leaves powder were mixed with of suitable solution. Then, the mixtures were extracted with the soxhlet apparatus for 4 hours. The antibacterial effects of extracts were researched on pathogens, namely Staphylococcus aureus ATCC 25923, Staphylococcus aureus ATCC 25953, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Pseudomonas aeruginosa ATCC 9027, Bacillus cereus ATCC 7064, Bacillus subtilis ATCC 6633, and Salmonella enteritidis ATCC 13076 using disc diffusion methods. Gentamicin and cefotaxime were used as control for bacteria. To try to understand each plant extracts effect on the antioxidant methods, we studied the different methods as named radical scavenging activity (DPPH), metal chelating activity, ferric reducing antioxidant power assays. In the plant extracts, total phenolic and total flavonoid contents were determined by spectrophotometric methods due to investigating the effect of secondary metabolites on antioxidant activity. To explore the beneficial effect of the extracts on hydroxyl radical-mediated DNA damage plasmid DNA pUC18 was used.

As a result, Arbutus andrachne extracts had antibacterial effect when they compared with control group antibiotics. According to disc diffusion methods, the highest antibacterial effect was identified in ethanol, chloroform and hexane extract, respectively. Ethanol extracts of Arbutus andrachne showed the best antioxidant activity. Moreover, extracts of Arbutus andrachne had repair effects on plasmid DNA in H₂O₂ condition.

The investigated Arbutus andrachne extract showed significant bioactivities. These extracts have been a promising candidate for the preparation of new natural products. However, future studies should be carried out to verify such actions in different matrices.

Keywords

• Arbutus andrachne L. antioxidant,antimicrobial,DNA interaction

Kaynakça

1. Apak, R., Özyürek, M., Güçlü, K., Çapanoğlu, E. (2016). Antioxidant activity/capacity measurement. 1. Classification, physicochemical principles, mechanisms, and electron transfer (ET)-based assays. Journal of agricultural and food chemistry, 64(5): 997-1027.
2. Ayvaz, M.Ç., Ömür, B., Ertürk, Ö., Kabakçı, D. (2018). Phenolic profiles, antioxidant, antimicrobial, and DNA damage inhibitory activities of chestnut honeys from Black Sea Region of Turkey. Journal Food Biochemistry, 2018: 1-10.
3. Asmae, E., Amina, C.H., Hakima, S., Abdellatif, H., Abdeslam, E., Saad, I., Mohamed, L., Mohammed, L. (2012). Extra- and intracellular antimycobacterial activity of Arbutus unedo L. African Journal of Microbiolgy Research 6: 1283–1290.
4. Baytop, T. (1999). Therapy with Medicinal Plants in Turkey (Past and Present), Nobel Medical Press, Istanbul.
5. Beattie, J., Crozier. A., Duthie, G.G. (2005). Potential health benefits of berries. Current Nutrition & Food Science. 1: 71.
6. Bilto, Y.Y., Alabdallat, N.G., Salim, M. (2015). Antioxidant properties of twelve selected medicinal plants commonly used in Jordan. British Journal of Pharmaceutical Research, 6(2): 121-130.
7. Bouyahya, A., Moussaoui, N.E., Abrini, J., Bakri, Y., Dakka, N. (2016). Determination of Phenolic Contents, Antioxidant and Antibacterial Activities of Strawberry Tree (Arbutus unedo L.) Leaf Extracts. British Biotechnology Journal, 14(3): 1-10.
8. Brand-Williams, W., Cuvelier, M.E., Berset, C. (1995). Use of a Free Radical Method to Evaluate Antioxidant Activity. LWT—Food Science Technology, 28: 25–30.

9. Clinical and Laboratory Standards Institute (CLSI) (2010). Performance Standards for Antimicrobial Susceptibility Testing; Twentieth Informational Supplement. M100-S20, Vol:30 No: 1.
10. Decker, E.A., Welch, B. (1990). Role of Ferritin as a Lipid Oxidation Catalyst in Muscle. Food Journal Agriculture Food Chemistry, 38: 674–677.
11. Delgado-Pelayo, R., Gallardo-Guerrero, L., Hornero-Méndez, D. (2016). Carotenoid composition of strawberry tree (Arbutus unedo L.) fruits Food Chemistry, 199: 165-175.
12. Ergun, N., Okmen, G., Yolcu, H., Cantekin, Z., Ergun, Y., Isık, D., Sengul, P. (2014). The enzymatic and non-enzymatic antioxidant activities of Arbutus andrachne L. leaf and flower and its antibacterial activities against mastitis pathogens. European Journal of Experimental Biology, 4(1): 227-232.
13. Erkekoglou, I., Nenadis, N., Samara, E., Mantzouridou, F.T. (2017). Functional teas from the leaves of Arbutus unedo: phenolic content, antioxidant activity, and detection of efficient radical scavengers. Plant Foods Human for Nutrition, 72: 176-183.
14. Ertabaklar, H., Kivçak, B., Mert, T., Ozensoy, T.S. (2009). In vitro activity of Arbutus unedo leaf extracts against Trichomonas vaginalis trophozoites. Acta Parasitology, 33: 263–265.
15. Ferreira, S., Santos, J., Duarte, A., Duarte, A.P., Queiroz, J.A., Domingues, F.C. (2012). Screening of antimicrobial activity of Cistus ladanifer and Arbutus unedo extracts. Natural Product Research, 26: 1558–1560.
16. Fonseca, D.F.S., Salvador, Â.C., Santos, S.A.O., Vilela, C., Freire, C.S.R., Silvestre, A.J.D., Rocha, S.M. (2015). Bioactive phytochemicals from wild Arbutus unedo L. Berries from different locations in portugal: quantification of lipophilic components. International Journal of Molecular Science, 16: 14194-14209.
17. Fortalezas, S., Tavares, L., Pimpão, R., Tyagi, M., Pontes, V., Alves, P., Santos, C. (2010). Antioxidant properties and neuroprotective capacity of strawberry tree fruit (Arbutus unedo). Nutrients, 2(2): 214-229.
18. Gül, M., Cali, İ.Ö., Cansaran, A., İdil, Ö., Kulu, İ., Çelikoğlu, U. (2017). Evaluation of phytochemical content, antioxidant, antimicrobial activity and DNA cleavage effect of endemic Linaria corifolia Desf. (Plantaginaceae). Cogent Chemistry, 3: 1-14.
19. Malheiroa, R., Saa, O., Pereiraa, E., Aguiarb, C., Baptistaa, P., Pereiraa, J.A. (2012). Arbutus unedo L. leaves as source of phytochemicals with bioactive properties. Industrial Crops and Products, 37: 473-478.
20. Morgado, S., Morgado, M., Plácido, A.I., Roque, F., Duarte, A.P. (2018). Arbutus unedo L. From traditional medicine to potential uses in modern Pharmacotherapy. Journal of Ethnopharmacology, 225: 90-102.
21. Oliveira, I., Coelho, V., Baltasar, R., Pereira, J.A., Baptista, P. (2009). Scavenging capacity of strawberry tree (Arbutus unedo L.) leaves on free radicals. Food and Chemical Toxicology, 47(7): 1507-1511.
22. Oyaizu, M. (1986). Studies on Product of Browning Reaction Prepared from Glucose Amine. The Japanese Journal of Nutrition and Dietetics, 44: 307–315.
23. Özgen, M., Torun, A.A., Ercişli, S., Serçe, S. (2009). Changes in chemical composition, antioxidant activities and total phenolic content of Arbutus andrachne fruit at different maturation stages. Italian Journal of Food Science, 21(1): 65-71.
24. Pallauf, K., Rivas-Gonzalo, J.C., Del Castillo, M.D., Cano, M.P., de Pascual-Teresa, S. (2008). Characterization of the antioxidant composition of strawberry tree (Arbutus unedo L.) fruits. Journal of Food Composition and Analysis, 21(4): 273-281.
25. Park, Y.S., Jung, S.T., Kang, S.G., Heo, B.K.H., Arancibia-Avila, P., Toledo, F., Drzewiecki, J., Namiesnik, J., Gorinstein, S. (2008). Antioxidants and Proteins in Ethylenetreated Kiwifruits. Food Chemistry, 107: 640–648.
26. Saklani, A., Kutty, S.K. (2008). Plants-derived compounds inclinical trials. Drug Discovery Today, 13: 161–171.
27. Serçe, S., Özgen, M., Torun, A.A., Ercişli, S. (2010). Chemical composition, antioxidant activities and total phenolic content of Arbutus andrachne L. (Fam. Ericaceae) (the Greek strawberry tree) fruits from Turkey. Journal of Food Composition and Analysis, 23(6): 619-623.
28. Sıcak, Y., Eliuz, A.E.R. (2019). Determination of the phytochemical profile, in vitro the antioxidant and antimicrobial activities of essential oil from Arbutus andrachne L. wood growing in Turkey. Turkish Journal of Forestry, 20(1): 57-61. Slinkard, K., Singleton, V.L. (1977). Total Phenol Analyses: Automation and Comparison with Manual Methods. American Journal of Enology and Viticulture, 28: 49–55.
29. Tenuta, M.C., Tundis, R., Xiao, J., Loizzo., M.R., Dugay, A., Deguin, B. (2019). Arbutus species (Ericaceae) as source of valuable bioactive products. Critical Reviews in Food Science and Nutrition, 59(6): 864-881.
30. Wendakoon, C.N., Sakaguchi, M. (1995). Inhibition of Amino Acid Decarboxylase Activity of Enterobacter aerogenes by Active Components in SpicesJ. Food. Prot., 58, 280-283.
31. World Health Organization (WHO). 2002–2005. WHO medicine strategy. Geneva.