Assesment of PTCH1 promoter methylation in BCC carcinogenesis

Yıl 2014, Cilt: 19 Sayı: 2, 105 - 106, 05.12.2014

Derya Sayın Kocakap

Öz

Basal Cell Carcinoma (BCC) is the most common cancer in humans. It is known that PTCH1 mutations in Sonic Hedgehog pathway play significant role in BCC  pathogenesis. However  PTCH1 (Patched) inactivation through promoter methylation is still under investigation. In this study promoter methylation of the PTCH1 gene was analysed by Combined Bisulphite Restriction Analysis in 10 formalin-fixed paraffin-embedded (FFPE) BCC tissues. There was no methylation in any of the samples. Results suggest that PTCH1 promoter methylation is not effective in BCC carcinogenesis and FFPE tissues are not suitable for methylation analysis since 44 specimens were included in the study and only 10 of them gave result.

Anahtar Kelimeler

Basal Cell Carcinoma, PTCH1, methylation, COBRA

Kaynakça

• Lacour JP. Carcinogenesis of basal cell carcinomas: genetics and molecular mechanisms. Br J Dermatol 2002; 146: 17-19.
• Toyota M, Kopecky KJ, Toyota MO, et al. Methylation profiling in acute myeloid leukemia. Blood 2001; 97: 2823-2829.
• Cretnik M, Musani V, Oreskovic S, Leovic D, Levanat S. The Patched gene is epigenetically regulated in ovarian dermoids and fibromas, but not in basocellular carcinomas. Int J Mol Med 2007; 19: 875-883.
• Pan S, Dong Q, Sun LS, Li TJ. Mechanisms of inactivation of PTCH1 gene in nevoid basal cell carcinoma syndrome: modification of the two-hit hypothesis. Clin Cancer Res 2010; 16: 442-450. Heitzer E, Bambach I, Dandachi N, Horn M, Wolf P. PTCH promoter methylation at low level in sporadic basal cell carcinoma analysed by three different approaches. Exp Dermatol 2010; 19: 926-928.