Genişletilmiş Kapı Alan Etkili Transistör Tabanlı Mikrosensör ile pH ve Üre Tespiti

Öz

Burada, pH ve ürenin yüksek hassasiyette tespiti için genişletilmiş kapı alan-etkili transistör tabanlı (EGFET) mikrosensörler geliştirilmiştir. EGFET, bir mikroelektrot ile entegre edilerek oluşturulmuştur. Kısaca, EGFET-tabanlı pH mikrosensörü, mikroelektrot yüzeyine elektropolimerizasyon yoluyla polipirol (PPy) modifiye edilerek ve buna küçük, basit ve ucuz yarı iletken metal oksit alan-etkili transistör (MOSFET) entegre edilmesiyle geliştirilmiştir. EGFET-tabanlı üre mikrosensöründe ise, üreaz enzimi PPy içerisine mobilize edilmiştir. pH ve üre ölçümleri sırasıyla farklı pH değerlerine ve üre konsantrasyonlarına sahip solüsyonlarla yapılmıştır. Sonuçlar pH mikrosensörünün pH 5-12 aralığında 67 mV/pH gibi çok iyi bir hassasiyete sahip olduğunu göstermiştir. Benzer şekilde, EGFET-tabanlı üre mikrosensörü, 35.5 mV/decade üre ve 10 µA/decade üre hassasiyetleriyle birlikte 10-9 - 10-5 M üre aralığında doğrusal bir yanıt gösterdi. Bildirilen EGFET pH ve üre mikrosensörleri, biyomedikal alanda, özellikle yerel analizin gerekli olduğu uygulamalarda kullanım için büyük potansiyele sahiptir.

Anahtar Kelimeler

• EGFET
• pH
• EGFET
• pH
• üre
• üreaz
• polipirol
• elektropolimerizasyonu
• mikrosensör

Detection of pH and Urea with an Extended Gate Field-Effect Transistor Based Microsensor

Abstract

Here, extended gate field-effect transistor-based (EGFET) microsensors have been developed for high sensitive detection of pH and urea. EGFET was made by integrating with a microelectrode. Briefly, the EGFET-based pH microsensor was developed by modifying the surface of a microelectrode with polypyrrole (PPy) via electropolymerization and integrating it with a small, simple and inexpensive metal oxide semiconductor field-effect transistor (MOSFET). As for the EGFET-based urea microsensor, urease enzyme was immobilized in PPy. The measurements of pH and urea were made in solutions at different pH values and urea concentrations, respectively. The results showed that the pH microsensor had a very good sensitivity of 67 mV/pH in a pH range of 5-12. Similarly, the EGFET-based urea microsensor showed a linear response range of 10-9 to 10-5 M urea with sensitivities of 35.5 mV/decade urea and 10 µA/decade urea. The reported EGFET-based pH and urea microsensors have great potential for use in the biomedical field, especially in applications where local analysis is required.

Keywords

• EGFET
• pH
• urea
• urease
• polypyrrole
• electropolymerization
• microsensor.

Kaynakça

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