Cardaria draba'nın Farklı Kısımlarının Fenolik Bileşimi, İn-Vitro Antioksidan ve Enzim İnhibisyon Aktiviteleri

Öz

Cardaria draba (L.) Desv. subsp. draba (Brassicaceae) bitkisi geleneksel olarak farklı kültürlerde çeşitli rahatsızlıklar için kullanılmaktadır. C. draba'nın çiçek, yaprak, gövde ve köklerinden elde edilen metanol, etanol ve su gibi farklı solvent ekstrelerinin kimyasal bileşimleri ile antioksidan aktivitesi ve asetilkolinesteraz (AChE), butirilkolinesterazın (BChE), α-glukosidaz, α-amilaz ve tirozinaz enzimleri üzerindeki inhibisyon aktivitelerini araştırdık. Her ekstrenin fenolik profilini belirlemek için RP-HPLC tekniği kullanıldı. Ekstrelerin antioksidan aktiviteleri ise DPPH ve ABTS yöntemi, toplam fenol ve flavonoid içerik analizi ve demir şelatlama deneyi kullanılarak belirlendi. Enzim inhibitör potansiyeli 96-mikroplaka yöntemi ile değerlendirildi. Sonuçlara göre, çiçek ekstresi en yüksek toplam fenolik içeriği (64.32 µgGAE/ mg) gösterirken, yaprak etanol ekstresi ise tüm özütler arasında en yüksek toplam flavonoid içeriğine (141.47 µgQE/ mg) sahip olduğu tespit edilmiştir. Çiçeklerin metanol ve su ekstraktları en etkili DPPH süpürücü iken, çiçeklerin sulu ekstraktı ABTS süpürmede en aktifti. Ekstraktların α-glukosidaza karşı α-amilaz enziminden daha umut verici aktiviteye sahip olduğu gösterilmiştir. Çiçeğin metanolik özütü, anti-asetilkolinesteraz aktivitesi gösterdi ve gövdenin etanolik özü, 12 özüt arasında en iyi anti-bütirilkolinesteraz aktivitesi göstermiştir. Anti-tirozinaz aktivitesine gelince, kök ve yaprakların sulu ekstraktları için daha yüksek kojik asite eşdeğer değerleri bulundu. Sonuç olarak, bu çalışmadan elde edilen veriler, aktif bileşiklerin biyo-aktivite rehberli izolasyonu ve Cardaria draba'dan yeni ilaçların geliştirilmesi için temel niteliği taşımaktadır.

Anahtar Kelimeler

• Cardaria draba
• Antioksidan aktivite
• Enzim inhibisyon
• YBSK

Phenolic Composition, In-vitro Antioxidant and Enzyme Inhibition Activities of Cardaria draba Different Parts

Abstract

The plant Cardaria draba (L.) Desv. subsp. draba (Brassicaceae), is traditionally used for various ailments in different cultures. We investigated different solvent extracts such as methanol, ethanol, and water extracts obtained from flowers, leaves, stems, and roots of C. draba for their chemical composition, as well as antioxidant and enzyme inhibition activities of acetylcholinesterase (AChE), butyrylcholinesterase (BChE), α-glucosidase, α-amylase, and tyrosinase). RP-HPLC technique was used to determine the phenolic profile of each extracts. The antioxidant activities of the extracts were determined by using DPPH and ABTS method, total phenol and flavonoid content analysis, and iron chelating assay. Enzyme inhibitory potential was evaluated by 96-microplate method. According to the results, aqueous extract of flower demonstrated the highest total phenolic content (64.32 µgGAE/ mg), while leaf ethanol extract showed the highest total flavonoid content (141.47 µgQE/ mg) among all extracts. The methanol, and water extracts of flowers were the most effective DPPH scavenger while aqueous extract of flower was the most active on ABTS scavenging. It is shown that the extracts possess promising activity against α-glucosidase than α-amylase enzyme. The methanolic extract of flower showed anti-acetylcholinesterase activity, and the ethanolic extract of the stem exhibited the best anti-butyrylcholinesterase activity among the 12 extracts. As for the anti-tyrosinase activity, higher kojic acid equivalent values were found for the aqueous extracts of roots and leaves. In conclusion, the data obtained from this study may serve as the basis for the bioassay-guided isolation of active compounds and the development of novel drugs from Cardaria draba.

Keywords

• Cardaria draba
• Antioxidant activity
• Enzyme inhibition
• HPLC

Kaynakça

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