Saponaria officinalis L. Kullanılarak Çinko Oksit Nanopartiküllerin Biyojenik Sentezi, Karakterizasyon ve Antibakteriyel Aktiviteler

Öz

Nanopartiküllerin sergilediği biyoaktivite, dünya çapındaki araştırmacılar tarafından büyük ilgi görmektedir. Çinko oksit nanoparçacıklarının (ZnO NP'ler) fitojenik sentezi, düşük toksisitesi ve biyolojik potansiyeli nedeniyle çevre dostu bir yaklaşım olmuştur. Günümüzde ZnONP'ler; biyomedikal, atık su arıtma, çevre iyileştirme gibi çok yönlü yapısı ve kozmetik ürünlerde uygulanabilirliği nedeniyle araştırmalar da öne çıkmaktadır. Biyosentez yöntemleriyle üretilen ZnO'nun antimikrobiyal, antioksidan ve antikanser özelliklere sahip çeşitli nanoyapılar sergilediği bilinmektedir. Bu nedenle yeşil sentez yöntemleriyle üretilen ZnONP'lerin antioksidan ve antibakteriyel aktivitesi üzerine yapılan çalışmalar her geçen gün artmaktadır. Bu çalışmada, gıda endüstrisinde, ekmek yapımında ve kozmetik sektöründe sabun yapımında kullanılan yüksek polifenol içeriğine sahip Saponaria officinalis bitkisinin kök kısımları değerlendirilerek ZnONP'lerin biyosentezi gerçekleştirilmiştir. Sentezlenen Nanopartiküllerin karakterizasyonu UV-VIS absorpsiyon spektroskopisi, FT-IR, XRD ve SEM analizi ile gerçekleştirilmiştir. Bu Green-ZnO NP'lerinin test edilen bakteri suşlarına karşı antibakteriyel aktiviteye sahip olduğu gösterildi. Elde edilen sonuçlar, Saponaria officinalis bitki ekstraktının çinko nanoparçacıklarının sentezi için iyi bir biyolojik indirgeyici ajan olarak kullanılabileceğini göstermiştir.

Anahtar Kelimeler

• yeşil sentez
• ZnO
• nanopartiküller
• Saponaria officinalis
• antibakteriyel aktivite

Biogenic Synthesis of Zinc Oxide Nanoparticles Using Saponaria officinalis L., Characterisation and Antibacterial Activities

Abstract

The bioactivity exhibited by nanoparticles is of great interest by researchers worldwide. Phytogenic synthesis of zinc oxide nanoparticles (ZnO NPs) has been an environmentally friendly approach due to its low toxicity and biological potential. Today ZnONPs; researches are also prominent due to its versatile structure such as biomedical, wastewater treatment, environmental improvement and also its applicability in cosmetic products. It is known that ZnO produced by biosynthesis methods exhibits various nanostructures with antimicrobial, antioxidant and anticancer properties. For this reason, studies on the antioxidant and antibacterial activity of ZnONPs produced by green synthesis methods are increasing day by day. In this study, the biosynthesis of ZnONPs was carried out by evaluating the root parts of Saponaria officinalis plant with high polyphenol content used in the food industry, bread making and soap making in the cosmetics sector. Characterization of the Nanoparticles synthesiszed were performed by UV-VIS absorption spectroscopy, FT-IR, XRD and SEM analysis. These Green-ZnO NPs were demonstrated to posses antibacterial activity against bacteria strains tested. The results obtained showed that Saponaria officinalis plant extract can be used as a good bioreducing agent for the synthesis of zinc nanoparticles.

Keywords

• green synthesis
• ZnO
• nanoparticles
• Saponaria officinalis
• antibacterial activity

Kaynakça

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