Development of New SSR Markers in Lentil by Scanning AG and AC Enriched Libraries with TG and TC Repeats

Abstract

Simple sequence repeats (SSRs) markers are important molecular tools widely used in genetic and genomic research in plants. However, the number of SSR markers developed in lentils (Lens culinaris Medik.) so far, which is an economically important cool season legume plant, has been very limited. The lack of SSR markers is among the main factors limiting lentil molecular breeding studies. In this study, 288 clones belonging to the enriched libraries with AC and AG repeats were screened by TG and TC repeats to develop SSR markers in lentil plants and 15 new SSR markers were developed. A total of 18 alleles were generated from markers showing polymorphism, and the Lc-MCu54 marker was identified as the most polymorphic marker. These developed markers are capable of contributing to many molecular-based studies in lentil plants.

Keywords

• SSRs
• Lentil
• Marker development
• Enriched library

Mercimekte AG ve AC Tekrarları ile Zenginleştirilmiş Kütüphanelerin TG ve TC Tekrarları ile Taranarak Yeni SSR Markörlerin Geliştirilmesi

Öz

Simple sequence repeats (SSRs) markörler, bitkilerde genetik ve genomik araştırmalarda yaygın olarak kullanılan önemli moleküler gereçlerdir. Ancak ekonomik öneme sahip serin iklim baklagil bitkilerinden mercimekte (Lens culinaris Medik.) bitkisinde bugüne kadar geliştirilen SSR markörü sayısı oldukça sınırlı kalmıştır. SSR markörlerin bu eksikliği, mercimek moleküler ıslah çalışmalarını sınırlayan başlıca faktörler arasında yer almaktadır. Bu çalışmada, mercimek bitkisinde SSR markörü geliştirmek için AC ve AG tekrarlarınca zenginleştirilmiş kütüphanelere ait 288 klon TG ve TC tekrarlarınca taranarak yeni 15 adet SSR markörü geliştirilmiştir. Polimorfizm gösteren markörlerden toplamda 18 allel üretilmiş ve Lc-MCu54 markörü en polimorfik markör olarak belirlenmiştir. Geliştirilen bu markörler mercimek bitkisinde moleküler temelli pek çok çalışmaya katkı sağlayacak niteliktedir.

Anahtar Kelimeler

• SSRs
• Mercimek
• Markör geliştirme
• Zenginleştirilmiş kütüphane

Kaynakça

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