Fasulye Genotiplerinde Tuz ve Kuraklık Stresleri Altında VPE Gen Ailesinin Genom Çapında Analizi ve Karakterizasyonu

Öz

Vakuolar işleme enzimleri (Vacoular Processing Enzymes; VPE’ler) önemli sistein proteazlarıdır ve protein öncüllerinin işlenmesi ve olgunlaşmasında, bitki gelişiminde, yaşlanmasında ve bağışıklığında, programlanmış hücre ölümünün yanı sıra şeker birikiminde işlev görür. Ayrıca biyotik ve abiyotik streslere karşı yanıt vermede önemli roller oynarlar. Çalışmanın amacı Phaseolus vulgaris bitkisinde in siliko olarak gerçekleştirilen VPE gen ailesi üyelerinin genom çaplı analizini ve karakterizasyonu sağlamak ve belirlenen gen ailesi üyelerinin abiyotik stresler (tuz ve kuraklık) altında vermiş olduğu yanıtların değerlendirilmesidir. Çeşitli in siliko yöntemler kullanılarak P. vulgaris genomunda 5 adet Pvul-VPE gen ailesi üyesi tespit edildi. Bu gen ailesi üyeleri 13,97 kDa ile 55,11 kDa arasında değişen moleküler ağırlığa sahipken, 123 ile 493 arasında değişen aminoasit sayısına sahip olduğu tespit edildi. İzoelektirik noktaları 5,48 (Pvul-VPE-4) ile 9,16 (Pvul-VPE-3) arasındadır. Ayrıca Pvul-VPE genleri fasulye genomunun 1, 3, 9 ve 11. kromozomları üzerinde konumlandığı tespit edilmiştir. Pvul-VPE gen ailesi üyeleri arasında yapılan ekzon ve intron analizleri sonucunda, toplam 40 ekzon ve 35 intron içerdikleri tespit edildi. Yapılan filogenetik analizler sonucunda Pvul-VPE proteinleri Arabidopsis thaliana ve Glycine max türleri ile 4 ana grupta kümelenmiştir. Pvul-VPE-1 ve Pvul-VPE-3 genleri arasında segmental duplikasyon olduğu tespit edildi. Pvul-VPE genlerinin ifade profilleri ile yapılan analizlerde farklı ifade seviylerine sahip oldukları tespit edildi ve bitkinin büyümesinde, gelişiminde ve olgunlaşmasında, protein öncüllerinin işlenmesinde ve bitkide şeker birikimi gibi görevlerin yanı sıra abiyotik ve biyotik streslere karşı yanıt oluşturmada önemli görevler üstlendiği tespit edildi. Yapılan çalışmanın sonuçları, VPE gen ailesinin P. vulgaris türünde kuraklık ve tuz streslerine karşı yanıtta ilk defa gerçekleştirilmiş olup, bitki ve tarımsal biyoteknoloji ve moleküler biyoloji alanlarında literatür bakımından kaynak ve ilave bilgiler sağlayacaktır.

Anahtar Kelimeler

• VPE
• Genom Çaplı Analiz
• Fasulye
• Biyoinformatik
• Abiyotik Stres

Genome-Wide Analysis and Characterization of the VPE Gene Family under Salt and Drought Stress in Common Bean Genotypes

Abstract

Vacuolar processing enzymes (VPEs) are cysteine proteases which are involved in the processing and maturation of protein precursors, as well as plant growth, senescence, and immunity, programmed cell death, and sugar accumulation. They also play a key role in biotic and abiotic stress responses. The goal of this study is to perform genome-wide analysis and characterization of VPE gene family members in Phaseolus vulgaris in silico, as well as to assess the reactions of the determined gene family members to abiotic stimuli (salt and drought). 5 members of the Pvul-VPE gene family was discovered in the P. vulgaris genome using a variety of in silico approaches. Members of this gene family have molecular weights ranging from 13.97 kDa to 55.11 kDa, and amino acid lengths ranging from 123 to 493 have been discovered. Its isoelectric points range from 5.48 (Pvul-VPE-4) to 9.16 (Pvul-VPE-3). Additionally, the Pvul-VPE genes were discovered on chromosomes 1, 3, 9, and 11 of the common bean genome. Exon and intron analysis of members of the Pvul-VPE gene family revealed that they have a total of 40 exons and 35 introns. Pvul-VPE proteins have been found to be clustered in four main groups with Arabidopsis thaliana and Glycine max species as a result of the phylogenetic analysis. Between the Pvul-VPE-1 and Pvul-VPE-3 genes, segmental duplication was discovered. The results of this study, which was performed for the first time in the response to drought and salt stresses in the VPE gene family's P. vulgaris species, will be a valuable source of information in the disciplines of plant breeding, agricultural biotechnology and molecular biology.

Keywords

• VPE
• Genome Wide Analysis
• Common Bean
• Bioinformatics
• Abiotic Stress

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