Developing a Potentiometric Ractopamine-Selective Electrode and Its Application

Öz

In this study, a potentiometric ion-selective electrode (ISE) was developed in order to quantify ractopamine. Ractopamine is a phenylethanolamine with ß2-adrenergic agonist properties; was in fact developed for treating respiratory diseases; and is used as tocolytic, bronchodilator and heart tonic in veterinary and human medicine (1). Ion-pair of Ractopamine-Tetraphenylborate was synthesized. The ion pair that was synthesized was employed as ionophore in the structure of electrode membranes. For the optimization of the membranes, PVC membrane ion-selective electrodes were produced in various compositions using the synthesized ion pairs and potentiometric performance characteristics of these electrodes were explored. It was determined that the electrode with the composition of 5% Rac-TPB+32%PVC+62.6%DBF+0.4% KTpClPB (Potassium tetrakis (4-chlorophenyl borate) demonstrated the best potentiometric performance characteristics. The linear range, slope, limit of quantification, pH range, and response time of this electrode were determined to be 1.0×10-5-1.0×10-1 M, 51.0 mV/decade, 1.0×10-5 M, 3.0-5.0, and< 5s, respectively. The electrode has demonstrated a highlyrepeatable potentiometric response. It was applied in pigswill using ractopamine selective electrode.

Anahtar Kelimeler

• Ion-selective electrode
• quantification of ractopamine
• PVC membrane electrode

Potansiyometrik Raktopamin Seçici Elektrot Geliştirilmesi ve Uygulaması

Öz

Bu çalışmada, raktopamin miktarını belirlemek için potansiyometrik iyon seçici bir elektrot (ISE) geliştirildi. Raktopamin, aslında solunum yolu hastalıklarının tedavisi için geliştirilmiş ve veterinerlikte ve insan tıbbında tokolitik, bronkodilatör ve kalp tonik olarak kullanılmakta olan ß2-adrenerjik agonist özelliklere sahip bir feniletanolamindir (1). Raktopamin-Tetrafenilborat iyon çifti sentezlendi. Sentezlenen iyon çifti, elektrot membranının yapısında iyonofor olarak kullanıldı. Membranların optimizasyonu için, PVC membran iyon seçici elektrotları, sentezlenen iyon çiftleri kullanılarak çeşitli bileşimlerde üretildi ve bu elektrotların potansiyometrik performans özellikleri araştırıldı. % 5 Rak-TPB +% 32 PVC +% 62,6 DBF +% 0,4 KTpClPB (Potasyum tetrakis (4-klorofenil borat) bileşimine sahip olan elektrodun en iyi potansiyometrik performans özelliklerini gösterdiği tespit edildi. Bu elektrodun doğrusal çalışma aralığı, eğimi, tayin limiti, pH aralığı ve cevap zamanı aralığı sırasıyla 1.0 x 10-5-1.0 x 10-1 M; 51.0 mV; 1.0 x 10-5 M, 3.0-5.0 ve <5s olarak belirlendi. Elektrot son derece tekrarlanabilir bir potansiyometrik cevap gösterdi. Raktopamin seçici elektrodu domuz yemine uygulandı.

Anahtar Kelimeler

• İyon seçici elektrot
• raktopamin tayini
• PVC membran elektrot

Kaynakça

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