Isolation and Characterization of Growth Hormone I Gene from Antalya Culture Population of Rainbow Trout (Oncorhynchus mykiss, Walbaum 1792)

Abstract

In this study, the growth hormone I gene was isolated and characterized from the rainbow trout culture population reared in Antalya Province. For this purpose, 10 individuals from the culture population were sampled. Total DNA was isolated from the muscle tissue of fish. Primers used in the polymerase chain reaction were designed and the target gene region was amplified. Sequence analysis of the isolated gene was performed and it was determined that the gene consisted of 4591 nucleotides. Compared to the reference sequence, 54 nucleotide differences were found corresponding to 2 transitional, 14 transversional and 38 insertional bases. All nucleotide variations are located in the intron regions of the gene. When the putative hormone response elements (HRE) were evaluated, they were seen to be completely compatible with the previously reported sequence. The data obtained in this study can be used in phylogenetic and gene transfer studies.

Keywords

• Growth hormone gene
• rtghI
• HRE
• Rainbow trout
• Oncorhynchus mykiss

Gökkuşağı Alabalığı (Oncorhynchus mykiss, Walbaum 1792) Antalya Kültür Popülasyonu Büyüme Hormonu I Geni İzolasyonu ve Karakterizasyonu

Öz

Bu çalışmada, Antalya İli’nde yetiştirilen gökkuşağı alabalığı kültür popülasyonundan büyüme hormonu I geni izole ve karakterize edilmiştir. Bu amaçla kültür popülasyonundan 10 birey örnek olarak alınmıştır. Örneklenen balıkların kas dokularından toplam DNA izole edilmiştir. Polimeraz zincir reaksiyonunda kullanılmak üzere primerler dizayn edilmiş ve hedef gen bölgesi çoğaltılmıştır. İzole edilen genin dizi analizi yapılmış ve 4591 nükleotitten oluştuğu tespit edilmiştir. Referans diziye kıyasla 2 transisyonel, 14 transversiyonel ve 38 insersiyonel baza karşılık gelen 54 nükleotitten oluşan mutasyon bulunmuştur. Tüm nükleotit farklılıkları genin intron bölgelerinde tespit edilmiştir. Genin intron bölgelerinde yer alan putatif hormon yanıt elemanları (HRE) değerlendirildiğinde ise daha önce raporlanan dizi ile tamamen uyumlu olduğu görülmüştür. Elde edilen veriler filogenetik ve gen aktarım çalışmalarında kullanılabilecek niteliktedir.

Anahtar Kelimeler

• Büyüme hormonu geni
• rtghI
• HRE
• Gökkuşağı alabalığı
• Oncorhynchus mykiss

Kaynakça

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