Bazı Gernik Buğdayların iPBS Markörleri Kullanılarak Moleküler Karakterizasyonu

Öz

Toplam 21 buğday genotipi (emmer buğdayı olarak adlandırılan 16 Triticum dicoccum ve durum buğdayı olarak adlandırılan 5 Triticum durum) arasındaki genetik çeşitlilik 7 iPBS primeri kullanılarak araştırılmıştır. Mevcut çalışmada kullanılan iPBS'ler, buğday genotipleri için 136 banttan 134’ünü polimorfik olarak üretmiştir. Bu çalışmanın sonucunda genetik çeşitlilik parametrelerinin ortalaması; polimorfizm bilgi içeriği (PIC = 0.19), heterozigotluk (H = 0.23), polimorfizm oranı (P% = 97.92) ve polimorfizm (19.14) belirlenmiştir. Jaccard'ın çeşitlilik değeri, buğday genotipleri için ortalama 0.4677 olarak hesaplanmış ve 0.0222 ile 0.7843 arasında değiştiği saptanmıştır. AMOVA sonucuna göre gernik ve makarnalık buğdaylarındaki genetik çeşitlilik, popülasyon içinde %77 varyasyona sahip olduğu tespit edilmiştir. Öte yandan, popülasyonlar arasındaki genetik varyasyon %23 değeriyle orta düzeydeyde olduğu bulunmuştur. Popülasyonların genetik çeşitliliğini belirlemek için lokus başına ortalama alel sayısı (Na = 1.269), etkili alel sayısı (Ne = 1.31), Shannon bilgi indeksi (I = 0.29), Nei'nin genetik çeşitlilik seviyesi (h = 0.19) gibi parametrelerle hesaplanmıştır. Ayrıca, Nei'nin tarafsız genetik çeşitlilik seviyesi (uh) 0.218 olarak saptanmıştır. Bu çalışmadan elde edilen sonuçlara göre iPBS moleküler markörlerinin buğday genotipleri arasındaki genetik ilişkilerin belirlenmesinde faydalı olabileceğini göstermiştir.

Anahtar Kelimeler

• Gernik Buğday
• Ekmeklik Buğday
• Triticum dicoccum
• Triticum durum
• Genetik İlişki
• iPBS

Genetic Diversity of Emmer Wheats Using iPBS Markers

Öz

The genetic diversity among totally 21 wheat genotypes (16 Triticum dicoccum called emmer wheat and 5 Triticum durum called durum wheat) were investigated using 7 iPBS primers. iPBSs used in the current study generated 134 polymorphic from 136 bands for wheat genotypes. The mean of genetic diversity parameters were calculated such as; polymorphism information content (PIC= 0.19), heterozygosity (H= 0.23), polymorphism ratio (P%= 97.92) and average polymorphism (19.14). Jaccard's diversity index varied between 0.0222 and 0.7843 with a mean 0.4677 for wheat genotypes. According to the sum of AMOVA, genetic diversity in emmer and durum wheats was significantly high with 77% variation within the population. On the other hand, genetic variation among populations was moderate with 23% value. Genetic diversity of populations were calculated by parameters such as the mean number of alleles per locus (Na=1.269), effective allele number (Ne=1.31), Shannon information index (I=0.29), Nei’s genetic diversity level (h=0.19), and Nei’s unbiased genetic diversity level (uh=0.218). Results obtained from this study showed that iPBS molecular markers could be useful in determination of genetic relationships among wheat genotypes.

Anahtar Kelimeler

• Emmer Wheat
• Durum Wheat
• Triticum dicoccum
• Triticum durum
• Genetic Diversity
• iPBS

Kaynakça

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