Klinik Kimya Laboratuvarında Acil Durum Testlerinde Plazma Tüpü Kullanımı

Öz

Laboratuvarlarda gerçekleştirilen hızlı testler için ana hususlar hızlı ve doğru bir test sonucu elde etmektir. Hızlı testlerin serum yerine plazma kullanılarak gerçekleştirilmesini mümkün kılmak için artan sayıda cihaz ve yöntem geliştirilmiştir. Bu çalışmanın amacı, serum ve plazma örnek tüplerinin hızlı test sonuçlarını karşılaştırmaktır. 78 hastanın venöz kan örnekleri BD Vacutainer®SST ™ II Advance serum jeli ve Vacutainer®Barricor ™ plazma tüpüne toplandı. Randomizasyonun sağlanması için kan alma ve tüplerin aletlere yüklenmesi sırasında tüplerin örnekleme sıraları değiştirildi. Kan alma anından itibaren tüplerin ters çevrilmesi, tamamen doldurulması ve serum tüplerinin en az 30 dakika süreyle pıhtılaşması sağlandı. Klorür ve sodyum testlerinin iyi bir uyuma sahip olduğu ve kalan testlerin güçlü bir uyum düzeyine sahip olduğu belirlendi. Albümin, ALT, Amilaz, AST, Bilirubin, Direkt Bilirubin, BUN, Kalsiyum, Klorür, Kreatinin, Mg, Na, CK, CRP, Üre, BNP, troponin-T, D-dimer analizi her biri için benzerdi ve anlamlı bir fark yoktu . Plazma elde etmek için pıhtılaşma süresinın gerekmemesi hızlı testin isüresinin kısalmasına yardımcı olurken, plazma tüpünde serum tüp örneklerinde görülebilen fibrin oluşumunun olmaması ise örnek kalitesini ve test sonuçlarının doğruluğunu destekler. Serum elde etmek için gerekli numune transferinin neden olduğu hemoliz olasılığı nedeniyle serum tüpleri yerine plazma tüpleri kullanılabilir.

Anahtar Kelimeler

• Serum
• Plazma tüpü
• Hızlı test
• Pıhtılaşma

Use of Plasma Tube for the Emergency Tests in Clinical Chemistry Laboratory

Abstract

The major considerations for the STAT testing performed in laboratories are to obtain a fast and accurate test result. Increasing number of instruments and methods have been developed for making it possible to perform the STAT testing on plasma rather than serum. The aim of this study is to compare the STAT test results of the serum and plasma sample tubes. Venous blood samples of 78 patients were collected into BD Vacutainer®SST™II Advance serum gel and Vacutainer®Barricor™ plasma tube. The sampling orders of the tubes were switched during blood collection and loading the tubes into the instruments in order to ensure randomization. It was ensured the tubes were inverted, completely filled, and were allowed serum tubes to clot for a minimum of 30 minutes from the time of blood collection. It was determined that the chloride and sodium tests had a good concordance and the remaining tests had a strong level of concordance. Albumin, ALT, Amylase, AST, Bilirubin, Direct Bilirubin, BUN, Calcium, Chloride, Creatinine, Mg, Na, CK, CRP, Urea, BNP, troponin-T, D-dimer analysis was similar for both, with no significant difference. While it helps to improve TAT since no clotting time is required to obtain plasma it also supports sample quality and accurate test results with the absence of fibrin formation at plasma tube which is possible at serum tube samples. Plasma tubes may be used instead of serum tubes due to the possibility of hemolysis caused by sample transfer required to obtain serum.

Keywords

• Serum
• Plasma tube
• STAT testing
• Clotting

Kaynakça

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