Foeniculum Vulgare Mill'in Uçucu Yağı İle Biyolojik Kontrol

Öz

Bu çalışmanın amacı, Cezayir'in Güney-Doğu bölgesinde hasat edilen Rezene'den (Foeniculum vulgare Mill.) hidrodistilasyon yoluyla elde edilen uçucu yağın kimyasal bileşimini araştırmak ve antifungal ve allelopatik aktivitelerini değerlendirmektir. Gaz kromatografisi-kütle spektrometrisi F. vulgare uçucu yağının GC-MS analizi, 11 bileşenin varlığını göstermiş ve alfa.-phellandrene (%29.44) ana bileşen olarak bulunmuştur. Uçucu yağ, 2 mahsulün (Chenopodium quinoa Willd.) Çeşit Q102 ve (Triticum durum Desf.) çeşidinin vitron tohumları üzerinde test edilmiştir. Kontrol amaçlı karşılaştırıldığında F. vulgare esansiyel yağı ile nihai çimlenme yüzdeleri ve fide sürgün ve kök uzunlukları önemli ölçüde azaltılmıştır. Ayrıca, Aspergillus niger ve Aspergillus flavus mantarları üzerinde yapılan antifungal çalışma önemli sonuçlar bildirmiştir. Yağın etkinliği, iki suşa karşı mantar öldürücü bir etkiye sahip olduğunu göstermiştir. Bu bulgular, F. vulgare esansiyel yağının biyolojik mücadelede çevre dostu bir kaynak olarak kullanılabileceğini düşündürmektedir.

Anahtar Kelimeler

• Allelopathie
• Antifungal aktivite
• Foeniculum vulgare Mill.
• Uçucu yağ.

Biological control with essential oil of Foeniculum vulgare Mill

Öz

The present study was aimed to investigate, the chemical composition of the essential oil extracted by hydrodistillation from Fennel (Foeniculum vulgare Mill.) harvested in South-Est, Algeria, and to evaluate its antifungal and allelopathic activities. Gas chromatography-mass spectrometry GC-MS analysis of F. vulgare essential oil showed the presence of 11 components, alpha.-phellandrene (29.44%) was found as the main component. The essential oil was tested on the seeds of 2 crops (Chenopodium quinoa Willd.) Variety Q102 and (Triticum durum Desf.) variety vitron, The final germination percentages and the seedling shoot and root lengths were significantly reduced by the F. vulgare essential oil as compared to the control. Furthermore, the antifungal study on the fungi of Aspergillus niger and Aspergillus flavus reported significant results. The effectiveness of the oil showed that it has a fungicidal effect against the two strains. These findings suggest that the F. vulgare essential oil can be used as a source of environmental-friendly in bilogical control.

Anahtar Kelimeler

• Allelopathie
• Antifungal activity
• Foeniculum vulgare Mill.
• Essential oil.

Destekleyen Kurum

(CRAPC), Ouargla

Teşekkür

The autors thank all the staff working in the technical floor for physical and chemical analyzes (CRAPC), Ouargla

Kaynakça

1. Anwar F., Ali M., Hussain AI, Shahid M., Antioxidant and antimicrobial activities of essential oil and extracts of fennel (Foeniculum vulgare Mill.) seeds from Pakistan. Flavour and Fragrance Journal 24, 170-176, 2009.
2. Alves, M.C.S.; Medeiros Filho, S.; Manoel Neto, A.; Brito, R.C.; Araujo, R.C. Allelopathic effect of essential oils of medicinal plants in Bidens pilosa L., Rev. Bras. Pl. Med., Campinas, v.16, n.3, supl. I, p.731-736, 2014.
3. Asma El Ayeb, Hichem Ben Jannet , Fethia Harzallah-Skhiri, Effects of Acacia cyanophylla Lindl. extracts on seed germination and seedling growth of four crop and weed plants. Turk J Biol. 37: 305-314,2014.
4. Bahmani, K., Darbandi, A.I., Ramshini, et al., Agro-morphological and phytochemical diversity of various Iranian fennel landraces. Ind. Crops Prod. 77, 282–294, 2015.
5. Chung IM, Ahn JK, Yun SJ. Assessment of allelopathic potential of barnyard grass (Echinochloa crus-galli) on rice (Oryza sativa L.) cultivars. Crop Prot 20: 921–928, 2001.
6. Farukh Sharopov, Abdujabbor Valiev, Prabodh Satyal et al., Cytotoxicity of the Essential Oil of Fennel (Foeniculum vulgare) from Tajikistan, Foods, 6, 73; doi:10.3390/foods6090073 ,2017. Gilani SA, Fujii Y, Shinwari ZK, Adnan M, et al., Phytotoxic studies of medicinal plant species of Pakistan. Pakistan Journal of Botany 42, 986-996, 2010.
7. Gholami Zali, A., Ehsanzadeh, P., Szumny, A., et al., Genotype-specific response of Foeniculum vulgare grain yield and essential oil composition to proline treatment under different irrigation conditions. Ind. Crops Prod. 124, 177–185, 2018.
8. Kucukboyac N, Ozkan S, Adıguzel N et al. Characterisation and antimicrobial activity of Sophora alopecuroides L. var. alopecuroides alkaloid extracts. Turk J Biol 35: 379–385, 2011.

9. Liman R, Eren Y, Akyıl D et al. Determination of mutagenic potencies of aqueous extracts of Thermopsis turcica by Ames test. Turk J Biol 36: 85–92, 2012.
10. Maryam Nourimand, Sasa Mohsenzadeh, Jaime A., Allelopathic Potential of Fenn el (Foeniculum vulgare Mill.), Medicinal and Aromatic Plant Science and Biotechnology 5 (1), 54-57, 2011.
11. Marotti, M., Piccaglia, R., Giovanelli et al. Effects of variety and ontogenic stage on the essential oil composition and biological activity of fennel (Foeniculum vulgare Mill.). Journal of Essential OilResearch, 6(1), 57-62, 1994.
12. Mimica-Dukic, N.; Kujundyic, S.; Sokovic, M. Et al., Essential oil composition and antifungal activity of Foeniculum vulgare Mill. Obtained by different distillation conditions. Phytother. Res., 17, 368–371, 2003. Mohamed Bilal Goudjil, Souad Zighmi, Djamila Hamada et al. Biological activities of essential oils extracted from Thymus capitatus (Lamiaceae), South African Journal of Botany 128: 1-9,2020.
13. Mustapha Abdellaoui, Eimad dine Tariq Bouhlali, Mgal Derouich, Essential oil and chemical composition of wild and cultivated fennel (Foeniculum vulgare Mill.): A comparative study, South African Journal of Botany 135 93-100, 2020.
14. Muckensturm, B., Foechterlen, D., Reduron, J. P., et al. Phytochemical and chemotaxonomic studies of Foeniculum vulgare. Biochemical systematics and ecology, 25(4), 353-358,1997.
15. Piccaglia, R., & Marotti, M. Characterization of some Italian types of wild fennel (Foeniculum vulgare Mill.). Journal of Agricultural and Food Chemistry, 49(1), 239-244, 2001.
16. Rahmani, M., [Investigation on the essential oil from the moroccan bitter fennel (Foeniculum vulgare Miller, var. Vulgare Miller thellung)]. [French]. Actes de l'Institut Agronomique et Veterinaire Hassan II 6, 5–11, 1986.
17. Shahat, A.A., Hammouda, F.M., Shams, K.A., et al., Comparative chemical analysis of the essential oil of wild and cultivated Fennel (Foeniculum vulgare Mill). J. Essent. Oil Bear. Plants 15, 314–319, 2012.
18. Singh HP., Batish DR., Kaur S., Kohli RK., Phytotoxic interference of Ageratum conyzoides with wheat (Triticum aestivum). Journal of Agronomy and Crop Science 189, 341-346, 2003.