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Dorystoechas hastata 'nın Kallus Kültürlerinin Oluşturulmasında Eksplant Tipi ve Bitki Büyüme Düzenleyicilerinin Etkisi

Yıl 2019, Sayı: 16, 367 - 373, 31.08.2019
https://doi.org/10.31590/ejosat.554947

Öz

Bu çalışmanın amacı monotipik endemik Dorystoechas hastata bitkisinin kallus kültürlerinin oluşturulmasında eksplant tipi ve bitki büyüme düzenleyicilerinin etkisinin belirlemektir. Doğal ve in vitro elde edilmiş bitkilerin çeşitli kısımları tek başına BA veya 2,4-D ve kombinasyonlarını içeren ve hiçbir bitki büyume düzenleyici içermeyen 1/2 MS besi ortamlarına alınarak uygun eksplant tipi ve büyüme düzenleyici konsantrasyonu belirlenmiştir. Doğal ortamından toplanan yaprak, yaprak sapı ve in vitro fidelerden elde edilen yaprak sapı eksplantları kültüre cevap vermemiştir. In vitro fidelerden elde edilen yaprak eksplantları sadece 2 mgL-1 BA içeren 1/2 MS ortamında kallus oluşturmuşlardır. Kotiledon eksplantları ise kültüre farklı oranlarda kallus indüksiyon frekansı oluşturarak cevap vermişlerdir. En yüksek kallus indüksiyon frekansı 1 mgL-1 BA ve 2 mgL-1 2,4-D içeren 1/2 MS ortamında elde edilmiştir (% 55). Dorystoechas hastata bitkisinin kallus kültürlerinin oluşturulmasında kotiledonlar en uygun eksplant olarak belirlenmiştir

Destekleyen Kurum

Aydın Adnan Menderes Üniversitesi Bilimsel Araştırma Projeleri Birimi

Proje Numarası

FEF-06014

Teşekkür

Bu çalışmanın gerçekleştirilmesi için tohum temin eden Doç. Dr. Özkan Eren’e ve finansal destek sağlayan Aydın Adnan Menderes Üniversitesi Bilimsel Araştırma Projeleri Birimine (Proje no: FEF-06014) teşekkür ederiz. Bu çalışmanın bir kısmı, 23-27 Haziran 2008 tarihleri arasında Trabzon-Türkiye de düzenlenen 19. Ulusal Biyoloji Kongresi'nde poster (PB 284) olarak sunulmuştur.

Kaynakça

  • Abeda, Z.H.J. 2015. Optimisation de la production d’anthocyanes par culture cellulaire d’oseille de Guinée [Hibiscus sabdariffa var sabdariffa L. (Malvaceae), isolement et étude de quelques activités biologiques. Thèse de Doctorat, Université Nangui Abrogoua.
  • Aliyu, O.M. 2005. Application of tissue culture to cashew (Anacardium occidentale) breeding: an appraisal. African J Biotech 4(13), 1485-1489.
  • Arafeh, R.M., Shibli, R.A., Al-Mahmoud, M., Shatnawi, M.A. 2006.Callusing, cell suspension culture and secondary metabolites production in Persian oregano (Origanum vulgare L.) and Arabian oregano (O. syriacum L.). Jordan J Agric Sci 2, 247-281.
  • Başer, K.H.C. 1994. Essential oil of Labiatae from Turkey-Recent, Lamiales Newsletter 3, 6-11.Baytop T. 1999.Türkiye’de Bitkiler ile Tedavi. Nobel yayınları, İstanbul. Bhojwani, S.S. Razdan, M.K. 1996. Plant Tissue Culture: Theory and Practice, a Revised Edition. Studies in Plant Science , 483-536, Elsevier.
  • Boix, Y.F., Arruda, R.C.O, Defaveri, A.C.A., Sato, A., Lage, C.L.S., Victo´rio, C.P. 2013. Callus in Rosmarinus officinalis L. (Lamiaceae): A morphoanatomical, histochemical and volatile analysis, Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology: Official Journal of the Societa Botanica Italiana, 147 (3), 751-757. Erdağ, B., Emek, Y., Kurt, S. 2010. Clonal propagation of Dorystoechas hastata via axillary shoot proliferation. Turkish Journal of Botany 34, 233-240.
  • Güner, A., Akyıldırım, B., Alkayış, M.F., Çıngay, B., Kanoğlu, S.S., Özkan, A. M., Öztekin, M., Tuğ, G.N. 2012. Güner A, Aslan S, Ekim T, Vural M., Babaç M.T, (eds). Türkiye Bitkileri Listesi (Damarlı Bitkiler). İstanbul: Nezahat Gökyiğit Botanik Bahçesi ve Flora Araştırmaları Derneği Yayını. İstanbul.
  • Hamid, B., Ouiam, C., Dambier, D., Froelicher, Y. 2012. Mise au point des conditions de calogenèse, caulogenèse et rhizogenèse chez les porte-greffes d’agrumes à partir d’épicotyle: cas du citrange Troyer. J Appl Biosci 60, 4375-4387.
  • Harley, R.M., Atkins, S., Budantsev A.L., , P.D., Conn, B.J., Grayer, R., Harley, M.M. 2004. “Labiatae,” in Flowering Plants. Dicotyledons: Lamiales (except Acanthacea including Avicenniaceae), Kadereit, J.W. (eds),167-275, Springer, Berlin, Germany.
  • Hussain, S.M., Fareed, S., Ansari, S., Akhlaquer, R.M., Ahmad, Z.I., Saeed, M. 2012. Current approaches toward production of secondary plant metabolites. J Pharm Bioallied Sci 4(1), 10-20.
  • Karim, R., Chlyah, H., Badoc, A., Douira, A. 2005. Obtention de pieds néoformés suite à l’induction de cals embryogènes d’embryons zygotiques de blés par le borate de sodium et un extrait de Fusarium graminearum. Bull Soc Pharm Bord 144, 195-210.
  • Loredo-Carrillo, S.E., Santos-Díaz, M.L., Leyva, E., Santos-Díaz, M.S. 2013. Establishment of callus from Pyrostegia venusta (Ker Gawl.) Miers and effect of abiotic stress on flavonoids and sterols accumulation. Journal of Plant Biochemistry and Biotechnology 22(3), 312-318.
  • Molina, S.M. 2004. In vitro callus induction and plantsfrom stem and petiole explants of Salvia canariensis L. Plant Tiss Cult 14, 167-172.
  • Murashige, T., Skoog, F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Plant Physiology 15, 473-497.
  • Naghibi, F., Mosaddegh, M., Motamed, S.M., Ghorbani, A. 2005. Labiatae family in folk medicine in Iran: from ethnobotany to pharmacology. Iranian Journal of Pharmaceutical Research 2, 63-79.
  • Özyiğit, I.I., Kahraman, M.V. Ercan, O. 2007. Relation between explant age, total phenols and regeneration response in tissue cultured cotton (Gossypium hirsutum L.). African Journal of Biotechnology 6(1), 3-8.
  • Patnaik, J., Sahoo, S., Debata, B.K. 1997. Somatic embryogenesis and plantlet regeneration from cell suspension cultures of palmarosa grass (Cymbopogon martini). Plant Cell Rep 16, 430-434.
  • Poudyal, B.K. Dı, G., Zhang Y., Liu, J., Shi, Q. 2008. Studies on browning problem and phenols content on shoots of Yali, Aikansui and Abbe Fetel pears for in vitro culture. Front Agric China 2(3), 321-330.
  • Sharifi, S., Sattari, T.N, Zebarjadi, A., Majd, A., Ghasempour, H.R. 2012. Enhanced callus induction and high-efficiency plant regeneration in Tribulus terrestris L., an important medicinal plant. Journal of Medicinal Plants Research 6 (27), 4401-4408.
  • Taji, A.M., Williams, R.R. 1996. Overview of Plant Tissue Culture. In: Tissue Culture of Australian Plants: Past, Present and Future. Armidale, Australia,Taji, A.M. and Williams, R.R. (eds.), 1-15. University of New England Press.
  • Tan, S.H., Musa, R., Ariff, A., Maziah, M. 2010. Effect of plant growth regulators on a callus, cell suspension and cell line selection for flavonoid production from pegaga (Centella asiatica L. urban). Am J Biochem Biotechnol 6, 284-299.
  • Thomas, P., Ravindra, M.B. 1999. Shoot tip culture in mango: Influence of medium, genotype, explant factors, season and decontamination treatments on phenolic exudation, explant survival and axenic culture establishment. J Horticultural Sci 72(5), 713-722.
  • Venturella, P., Venturella, G., Marino, M. L., Mericli, A. H., Çubukcu, B. 1988. Phytochemical investigation of the Labiatea Dorystoechas hastata. Giornale Botanico Italiano 122, 291–294.
  • Vijaya, S.N., Udayasri, P.V., Aswani, K.Y., Ravi, B.B., Phani, K.Y., Vijay, V.M. 2010. Advancements in the production of secondary metabolites. J Nat Prod 3, 112-123.s

Development of Callus due to Explant Type and Plant Growth Regulators in Dorystoechas hastata Plant

Yıl 2019, Sayı: 16, 367 - 373, 31.08.2019
https://doi.org/10.31590/ejosat.554947

Öz

This study was planned to determine the effect of explant type and plant growth regulators in the formation of callus cultures of the monotypic endemic Dorystoechas hastata plant. Various parts of the plants collected from their natural population and obtained in vitro were cultured on 1/2 MS medium containing BA or 2,4-D alone and combinations of these growth regulators. 1/2 MS medium without plant growth regulators was determined as control medium. The explants collected from wild and petiol explants obtained from in vitro seedlings did not respond to culture. Leaf explants obtained from in vitro seedlings formed calli only on 1/2 MS medium with 2 mgL-1 BA. Cotyledon explants obtained from in vitro seedlings were responded to the culture by creating callus induction frequency at different rates. The highest callus induction frequency was obtained on 1/2 MS medium with 1 mgL-1 BA and 2 mgL-1 2,4-D (55%). At the end of the experiments, cotyledons were determined as the most suitable explants in the formation of callus cultures of Dorystoechas hastata.

Proje Numarası

FEF-06014

Kaynakça

  • Abeda, Z.H.J. 2015. Optimisation de la production d’anthocyanes par culture cellulaire d’oseille de Guinée [Hibiscus sabdariffa var sabdariffa L. (Malvaceae), isolement et étude de quelques activités biologiques. Thèse de Doctorat, Université Nangui Abrogoua.
  • Aliyu, O.M. 2005. Application of tissue culture to cashew (Anacardium occidentale) breeding: an appraisal. African J Biotech 4(13), 1485-1489.
  • Arafeh, R.M., Shibli, R.A., Al-Mahmoud, M., Shatnawi, M.A. 2006.Callusing, cell suspension culture and secondary metabolites production in Persian oregano (Origanum vulgare L.) and Arabian oregano (O. syriacum L.). Jordan J Agric Sci 2, 247-281.
  • Başer, K.H.C. 1994. Essential oil of Labiatae from Turkey-Recent, Lamiales Newsletter 3, 6-11.Baytop T. 1999.Türkiye’de Bitkiler ile Tedavi. Nobel yayınları, İstanbul. Bhojwani, S.S. Razdan, M.K. 1996. Plant Tissue Culture: Theory and Practice, a Revised Edition. Studies in Plant Science , 483-536, Elsevier.
  • Boix, Y.F., Arruda, R.C.O, Defaveri, A.C.A., Sato, A., Lage, C.L.S., Victo´rio, C.P. 2013. Callus in Rosmarinus officinalis L. (Lamiaceae): A morphoanatomical, histochemical and volatile analysis, Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology: Official Journal of the Societa Botanica Italiana, 147 (3), 751-757. Erdağ, B., Emek, Y., Kurt, S. 2010. Clonal propagation of Dorystoechas hastata via axillary shoot proliferation. Turkish Journal of Botany 34, 233-240.
  • Güner, A., Akyıldırım, B., Alkayış, M.F., Çıngay, B., Kanoğlu, S.S., Özkan, A. M., Öztekin, M., Tuğ, G.N. 2012. Güner A, Aslan S, Ekim T, Vural M., Babaç M.T, (eds). Türkiye Bitkileri Listesi (Damarlı Bitkiler). İstanbul: Nezahat Gökyiğit Botanik Bahçesi ve Flora Araştırmaları Derneği Yayını. İstanbul.
  • Hamid, B., Ouiam, C., Dambier, D., Froelicher, Y. 2012. Mise au point des conditions de calogenèse, caulogenèse et rhizogenèse chez les porte-greffes d’agrumes à partir d’épicotyle: cas du citrange Troyer. J Appl Biosci 60, 4375-4387.
  • Harley, R.M., Atkins, S., Budantsev A.L., , P.D., Conn, B.J., Grayer, R., Harley, M.M. 2004. “Labiatae,” in Flowering Plants. Dicotyledons: Lamiales (except Acanthacea including Avicenniaceae), Kadereit, J.W. (eds),167-275, Springer, Berlin, Germany.
  • Hussain, S.M., Fareed, S., Ansari, S., Akhlaquer, R.M., Ahmad, Z.I., Saeed, M. 2012. Current approaches toward production of secondary plant metabolites. J Pharm Bioallied Sci 4(1), 10-20.
  • Karim, R., Chlyah, H., Badoc, A., Douira, A. 2005. Obtention de pieds néoformés suite à l’induction de cals embryogènes d’embryons zygotiques de blés par le borate de sodium et un extrait de Fusarium graminearum. Bull Soc Pharm Bord 144, 195-210.
  • Loredo-Carrillo, S.E., Santos-Díaz, M.L., Leyva, E., Santos-Díaz, M.S. 2013. Establishment of callus from Pyrostegia venusta (Ker Gawl.) Miers and effect of abiotic stress on flavonoids and sterols accumulation. Journal of Plant Biochemistry and Biotechnology 22(3), 312-318.
  • Molina, S.M. 2004. In vitro callus induction and plantsfrom stem and petiole explants of Salvia canariensis L. Plant Tiss Cult 14, 167-172.
  • Murashige, T., Skoog, F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Plant Physiology 15, 473-497.
  • Naghibi, F., Mosaddegh, M., Motamed, S.M., Ghorbani, A. 2005. Labiatae family in folk medicine in Iran: from ethnobotany to pharmacology. Iranian Journal of Pharmaceutical Research 2, 63-79.
  • Özyiğit, I.I., Kahraman, M.V. Ercan, O. 2007. Relation between explant age, total phenols and regeneration response in tissue cultured cotton (Gossypium hirsutum L.). African Journal of Biotechnology 6(1), 3-8.
  • Patnaik, J., Sahoo, S., Debata, B.K. 1997. Somatic embryogenesis and plantlet regeneration from cell suspension cultures of palmarosa grass (Cymbopogon martini). Plant Cell Rep 16, 430-434.
  • Poudyal, B.K. Dı, G., Zhang Y., Liu, J., Shi, Q. 2008. Studies on browning problem and phenols content on shoots of Yali, Aikansui and Abbe Fetel pears for in vitro culture. Front Agric China 2(3), 321-330.
  • Sharifi, S., Sattari, T.N, Zebarjadi, A., Majd, A., Ghasempour, H.R. 2012. Enhanced callus induction and high-efficiency plant regeneration in Tribulus terrestris L., an important medicinal plant. Journal of Medicinal Plants Research 6 (27), 4401-4408.
  • Taji, A.M., Williams, R.R. 1996. Overview of Plant Tissue Culture. In: Tissue Culture of Australian Plants: Past, Present and Future. Armidale, Australia,Taji, A.M. and Williams, R.R. (eds.), 1-15. University of New England Press.
  • Tan, S.H., Musa, R., Ariff, A., Maziah, M. 2010. Effect of plant growth regulators on a callus, cell suspension and cell line selection for flavonoid production from pegaga (Centella asiatica L. urban). Am J Biochem Biotechnol 6, 284-299.
  • Thomas, P., Ravindra, M.B. 1999. Shoot tip culture in mango: Influence of medium, genotype, explant factors, season and decontamination treatments on phenolic exudation, explant survival and axenic culture establishment. J Horticultural Sci 72(5), 713-722.
  • Venturella, P., Venturella, G., Marino, M. L., Mericli, A. H., Çubukcu, B. 1988. Phytochemical investigation of the Labiatea Dorystoechas hastata. Giornale Botanico Italiano 122, 291–294.
  • Vijaya, S.N., Udayasri, P.V., Aswani, K.Y., Ravi, B.B., Phani, K.Y., Vijay, V.M. 2010. Advancements in the production of secondary metabolites. J Nat Prod 3, 112-123.s
Toplam 23 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Konular Mühendislik
Bölüm Makaleler
Yazarlar

Bengi Erdağ 0000-0001-7042-9011

Yelda Emek 0000-0003-1095-3908

Serap Kurt Aydoğan Bu kişi benim

Proje Numarası FEF-06014
Yayımlanma Tarihi 31 Ağustos 2019
Yayımlandığı Sayı Yıl 2019 Sayı: 16

Kaynak Göster

APA Erdağ, B., Emek, Y., & Kurt Aydoğan, S. (2019). Dorystoechas hastata ’nın Kallus Kültürlerinin Oluşturulmasında Eksplant Tipi ve Bitki Büyüme Düzenleyicilerinin Etkisi. Avrupa Bilim Ve Teknoloji Dergisi(16), 367-373. https://doi.org/10.31590/ejosat.554947