Yıl 2019, Cilt , Sayı 16, Sayfalar 776 - 784 2019-08-31

Development of a potentiometric ractopamine-selective electrode and its application


In this study, a potentiometric ion-selective electrode (ISE) was developed in order to quantifyractopamine. Ractopamine is a phenylethanolamine with ß2-adrenergic agonist properties; was in fact developed for treating respiratory diseases; and is used as tocolytic, bronchodilator and heart tonic in human and veterinary medicine (1). Ion-pair of Ractopamine-Tetraphenylborate was synthesized. The synthesized ion pair was used as ionophore in the structure of electrode membranes. For membrane optimization, PVC membrane ion-selective electrodes were produced in various compositions using the synthesized ion pairs and potentiometric performance characteristics of these electrodes were investigated. It was determined that the electrode with the composition of 5% Rac-TPB+32%PVC+62.6%DBF+0.4% KTpClPB (Potassium tetrakis (4-chlorophenyl borate) demonstrated the best potentiometric performance characteristics. The linear range, slope, limit of quantification, pH range, and response time of this electrode were determined to be 1.0×10-5-1.0×10-1 M, 51.0 mV/decade, 1.0×10-5 M, 3.0-5.0, and< 5s, respectively. The electrode has demonstrated a highlyrepeatable potentiometric response. It was applied in pigswill using ractopamine selective electrode.

Ion-selective electrode, quantification of ractopamine
  • Blanca, J., Munoz, P., Morgado, M., Mendez, N., Aranda, A., Reuvers, T., & Hooghuis, H. (2005). Determination of clenbuterol, ractopamine and zilpaterol in liver and urine by liquid chromatography tandem mass spectrometry. Analytica Chimica Acta, 529, 199–205.
  • Bolera, D. D., Shrecka, A. L., Faulknera, D. B., Killefera, J., McKeitha, F. K., Hommb, J. W. & Scanga, J. A. (2012). Effect of ractopamine hydrochloride (Optaflexx) dose on live animal performance, carcass characteristics and tenderness in early weaned beef steers. Meat Science, 92, 458–463.
  • Brambilla, G., T. Cenci, F. Franconi, R. Galarini, A. Macrì, F. Rondoni, M. Strozzi, A. & Loizzo, (2000). Clinical and pharmacological profile in a clenbuterol epidemic poisoning of contaminated beef meat in Italy, Toxicology Letters, 114, 47–53.
  • Dong, J. X., Li, Z. F., Lei, H. T., Sun, Y. M., Ducancel, F., Xu, Z. L., Boulain, J. C., Yang, J. Y., Shen, Y. D., & Wang, H. (2012). Development of a single-chain variable fragmentalkaline phosphatase fusion protein and a sensitive direct competitive chemiluminescent enzyme immunoassay for detection of ractopamine in pork. Analytica Chimica Acta, 736, 85–91.
  • Halsey, C. H. C., Weber, P. S., Reiter, S. S., Stronach, B. N., Bartosh, J. L., & Bergen, W. G. (2011). The effect of ractopamine hydrochloride on gene expression in adipose tissues of finishing pigs. Journal of Animal Science, 89, 1011–1019.
  • He, L. M., Su, Y. J., Zeng, Z. L., Liu, Y. H., & Huang, X. H. (2007). Determination of ractopamine and clenbuterol in feeds by gas chromatography–mass spectrometry. Animal Feed Science and Technology, 132, 316–323.
  • Lu, X., Zheng, H., Li, X. Q., Yuan, X. X., Li, H., Deng, L. G., Zhang, H., Wang, W. Z., Yang, G. S., Meng, M., Xi, R. M., & Aboul-Enein, H. Y. (2012). Detection of ractopamine residues in pork by surface plasmon resonance-based biosensor inhibition immunoassay. Food Chemistry, 130, 1061–1065.
  • Pleadin, J., Perši, N., Vulic´ , A., Milic´ , D., & Vahcˇic´ , N. (2012). Determination of residual ractopamine concentrations by enzyme immunoassay in treated pig’s tissues on days after withdrawal. Meat Science, 90, 755–758.
  • Shen, L.,& He, P. L. (2007). An electrochemical immunosensor based on agarose hydrogel films for rapid determination of ractopamine. Electrochemistry Communications, 9, 657–662.
  • Turberg, M. P., Rodewald, J. M., & Coleman, M. R. (1996). Determination of ractopamine in monkey plasma and swine serum by high-performance liquid chromatography with electrochemical detection. Journal of Chromatography B, 675, 279– 285.
  • Von Haehling, S., Lainscak, M., Springer, J. & Anker, S.D. (2009). Cardiac cachexia: a systematic overview, Pharmacology & Therapeutics, 121, 227–252.
  • Wang, W. Y., Zhang, Y. L., Wang, J. Y., Shi, X. S., & Ye, J. N. (2010). Determination of ß-agonists in pig feed, pig urine and pig liver using capillary electrophoresis with electrochemical detection. Meat Science, 85, 302–305.
Birincil Dil en
Konular Mühendislik
Bölüm Makaleler

Orcid: 0000-0003-4231-6217
Yazar: Gülşah SAYDAN KANBEROĞLU (Sorumlu Yazar)
Kurum: Van Yüzüncü Yıl Üniversitesi
Ülke: Turkey

Destekleyen Kurum Van Yüzüncü Yıl Üniversitesi
Proje Numarası FBA-2018-7568
Teşekkür Van Yüzüncü Yıl Üniversitesi Bilimsel Araştırma Projeleri Birimine teşekkür ederiz.

Yayımlanma Tarihi : 31 Ağustos 2019

APA Saydan Kanberoğlu, G . (2019). Development of a potentiometric ractopamine-selective electrode and its application . Avrupa Bilim ve Teknoloji Dergisi , (16) , 776-784 . DOI: 10.31590/ejosat.585768