Bean Common Mosaic Virus and Bean Common Mosaic Necrosis Virus Infections in Bean Production Areas in The Lakes Region of Turkey

Yıl 2020, Sayı: 19, 386 - 392, 31.08.2020

Handan Çulal Kılıç Hesna Kök Necla Yardımcı

https://doi.org/10.31590/ejosat.705686

Cited By: 3

Öz

Common bean, belonging to family Leguminosae (Fabaceae), has an important place in people nutrition for thousands of years. Although many viruses infect bean plants, economically most important ones are potyviruses. Among them, BCMV (Bean common mosaic virus) and BCMNV (Bean common mosaic necrosis virus) are the most common viruses in common bean production areas all over the world. During 2014-2015 growing season, total of 361 bean samples (275 leaf and 86 seed) were collected from several localities in the Lakes Region. According to the results of DAS-ELISA (Double Antibody Sandwich Enzyme Linked Immunusorbent Assay) test, percentages of the infections of BCMV and BCMNV in the leaf samples were 26.18% and 15.27%, respectively. In seed samples, BCMV and BCMNV infection rates were 2.32% and 6.97%, respectively. Percentages of the mixed infections of the viruses in the leaf samples were 25.45%, while that in the seed samples were. 55.81%. In the mechanically inoculation studies where two isolates (BCMV-I and BCMN-BT) were used, typical symptoms of the viruses were observed. In molecular studies, a 850 bp fragment of the CP (Coat Protein) gene was amplified by IC-RT-PCR (Immunocapture Reverse Transcription Polimerase Chain Reaction) using BCMV specific primer pairs, but amplification of the CP region of RNA genome of BCMNV could not be succeeded. This is first report of biological and serological methods in Lakes region.  

Anahtar Kelimeler

Bean, BCMV, BCMNV, Detection

Göller Bölgesi Fasulye Üretim Alanlarında Fasulye Adi Mozayik ve Fasulye Adi Mozayik Nekroz Virüs’ü Enfeksiyonları

Öz

Fasulye binlerce yıldır insan beslenmesinde önemli bir yeri olan Leguminosae (Fabaceae) familyası bitkisidir. Fasulye bitkisi çok sayıda virüs hastalığından önemli miktarda etkilense de ekonomik olarak en önemli zararları Potyvirüsler yapmaktadır. Bunların içerisinde en önemlileri Fasulye adi mozayik virüs (BCMV) ve Fasulye adi nekroz mozayik virüsü (BCMNV)’dür. Bu çalışma da, 2014-2015 yıllarında Göller Bölgesi fasulye üretim alanlarından 275 yaprak ve 86 tohum örneği olmak üzere toplam 361 bitki örneği toplanmıştır. DAS-ELISA metoduna göre; alınan yaprak örneklerinde BCMV’nün enfeksiyon yüzdesi, %26.18 iken; BCMNV’nün %15.27’dir. Tohum örneklerinde ise BCMV’nün enfeksiyon yüzdesi %2.32; BCMNV’nün %6.97’dir.Karışık enfeksiyon yüzdesi yaprak örneklerinde %25.45 iken tohumlarda bu oran %55.81 olarak belirlenmiştir. Mekanik inokulasyon çalışmalarında (BCMV-I ve BCMN-BT) iki izolat kullanılmıştır. Çalışmaların sonucunda virüslere özgü tipik simptomlar gözlenmiştir. Moleküler çalışmalarda; BCMV’ne özgü primer çiftleri ile kılıf protein geninin 850 bp’lik bir kısmı IC-RT-PCR (Immunocapture Reverse Transcription Polimerase Chain Reaction) metoduyla çoğaltılabilirken, BCMNV’nün amplifikasyonunda başarı elde edilememiştir.

Anahtar Kelimeler

Fasulye, BCMV, BCMNV, Teşhis

Kaynakça

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