Determination Of Ciprofloxacin In Pharmaceutical Dosage, Human Serum and Urine, Using Molecularly Imprinted Polymer Modified Electrode By Voltammetry

Yıl 2020, Sayı: 20, 859 - 865, 31.12.2020

Berrin Gürler Akyüz , Sabriye Perçin Özkorucuklu , Esengül Kır , Gizem Yıldırım Baştemur

https://doi.org/10.31590/ejosat.796654

Öz

Molecularly imprinted polymer based working electrode was prepared for the determination of ciprofloxacin (CF). Electrochemical behavior of CF was investigated by differential pulse voltammetric (DPV) method in the range of 10-60% (v/v) acetonitrile (MeCN)-H2O binary mixture at the pH between 3-7 prepared Britton-Robinson (BR) buffers. Voltammetric analyzes were performed with molecularly imprinted (MIP) and non-imprinted (NIP) modified electrodes. The influence of the electropolymerization cycles, solution pH, and MeCN-H2O ratio, on the performance of both electrodes were evaluated and optimized. Detection limit was obtained as 3.34×10-5 M (S/N=3). Recommended method was successfully employed for determination of CF in pharmaceutical, human serum and urine.

Anahtar Kelimeler

Ciprofloxacin, Polypyrrole, Biological sample, Differential puls voltammetry, Molecularly imprinting

Farmasötik Dozaj, İnsan Serumu ve İdrarındaki Siprofloksasinin Moleküler Baskılı Polipirol Modifiye Elektrot Kullanılarak Voltametrik Tayini

Öz

Siprofloksasin tayini için moleküler baskılanmış polimer bazlı çalışma elektrodu hazırlanmıştır. Siprofloksasinin elektrokimyasal davranışı % 10-60 (v/v) asetonitril -su ikili karışımında ve pH 3-7 aralığında hazırlanan Britton-Robinson tamponu kullanılarak diferansiyel puls voltametrisi yöntemiyle incelenmiştir. Voltametrik analizler moleküler baskılanmış (MIP) ve baskılanmamış (NIP) modifiye elektrotlar ile gerçekleştirilmiştir. Elektropolimerizasyon döngü sayısı, çözelti pH'sı ve asetonitril-su oranının her iki tip elektrodun performansı üzerindeki etkisi araştırılmış ve optimize edilmiştir. Dedeksiyon limiti 3.34×10-5 M (S/N = 3) olarak bulunmuştur. Önerilen yöntem ilaçta, insan kanı ve idrarındaki siprofloksasinin tayini için başarıyla uygulanmıştır.

Anahtar Kelimeler

Siprofloksasin, Polipirol, Biyolojik numune, Diferansiyel puls voltametri, Moleküler baskılama

Kaynakça

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