Erzincan İlinde İki Noktalı Kırmızı Örümcek [Tetranychus urticae Koch, 1836 (Acari: Tetranychidae)]’den Entomopatojenik Fungus İzolasyonu

Yıl 2021, Sayı: 21, 568 - 571, 31.01.2021

Engin Kılıç

Öz

Bu çalışma 2015-2017 yılları arasında yürütülmüştür. Kuru fasulye üretim alanlarından akarlarla bulaşık fasulye yaprakları toplanıp, polietilen torbabalara konularak laboratuvara getirilmiştir. Laboratuvarda bir fırça yardımıyla fasulye yapraklarındaki akarlar Patates Dekstroz Aagarlı (PDA) petrilere aktarılmıştır. Petriler fungusların gelişimi ve sporulasyonu için inkübatöre konulmuştur (2 hafta-25±1ºC). Tetranychus urticae Koch, 1836 (Acari:Tetranychidae) kadavralarından 6 adet entomopatojenik fungus (EPF) izolatı elde edilmiştir. Bunlardan 4’ü Beauveria bassiana (Balsamo) Vuillemin (Deuteromycetes: Hyphomycetes) ve 2’si Metharizium anisopliae (Metsch.) Sorokin (Deuteromycotina: Hyphomycetes) olduğu teşhis edilmiştir. Teşhisten sonra izolatlar kodlanmış ve daha sonraki çalışmalar için Erzincan Binali Yıldırım Üniversitesi, Eczacılık Fakültesi, Farmasötik Mikrobiyoloji Laboratuvarında buzdolabı şartlarında depolanmıştır.

Anahtar Kelimeler

Beauveria bassiana, Metharizium anisopliae, Tetranychus urticae, Erzincan

Destekleyen Kurum

Erzincan Binali Yıldırım Üniversitesi

Proje Numarası

FEN-A-300614-0104

Teşekkür

Erzincan Binali Yıldırım Üniversitesi BAP birimine teşekkür ederim

Isolation Entomopathogenic fungi From The Two-Spotted Spider Mite (Teranychus urticae) at Erzincan Provience

Öz

This study was conducted between 2015-2017. The infested with mites of the bean leaves were collected from the field. Then put in polyethylene bags, and brought to the laboratory. The mites on the bean leaves were transferred to the petri dish with Potato Dexrose Aagar (PDA) by a brush at the laboratory. The petri dishes were incubated for growth and sporulation of the fungus (2 weeks in the incubator at 25±1ºC). As a result, six entomopathogenic fungi (EPF) ısolates were isolated from the cadaver of the Tetranychus urticae Koch, 1836(Acari: Tetranychidae) Four of them were Beauveria bassiana (Balsamo) Vuillemin (Deuteromycetes: Hyphomycetes) and two of them were Metharizium anisopliae (Metsch.) Sorokin (Deuteromycotina: Hyphomycetes). After the identification of isolates, they have been coded and stored under refrigerator conditions for use in other studies at the Pharmaceutical Microbiology Laboratory of the Faculty of Pharmacy, Erzincan Binali Yıldırım University.

Anahtar Kelimeler

Beauveria bassiana, Metharizium anisopliae, Tetranychus urticae, Erzincan

Proje Numarası

FEN-A-300614-0104

Kaynakça

• Alves SB, Tamai MA, Lopes RB (1998) Avaliação de Beauveria bassiana (Bals.) Vuill. para controle de Tetranychus urticae Koch em crisântemo. In: Abstracts 17th Brazil. congr entomol Rio de Janeiro, p 1068
• Alves SB, Rossi LS, Lopes RB, Tamai MA, Pereira RM (2002) Beauveria bassiana yeast phase on agar medium and its pathogenicity against Diatraea saccharalis (Lepidoptera: Crambidae) and Tetranychus urticae (Acari: Tetranychidae). J Invertebr Pathol 81:70–77
• Alves SB, Tamai MA, Rossi LS, Castiglioni E. 2005. Beauveria bassiana pathogenicity to the citrus rust mite Phyllocoptruta oleivora. Experimental and Applied Acarology 37: 117–122.Aydın, L., (2005). Varroa destructor’un kontrolünde yeni stratejiler’, Uludağ Arıcılık Dergisi, 5 pp. 59–62
• Balcı M.H.,İNANICI M. A., AY, R. (2020) Effects of Some Pesticides on Tetranychus urticae and Predatory Mite Phytoseiulus persimilis in Laboratory Conditions. Journal of Tekirdag Agricultural Faculty. Cilt 17, Sayı 2, Sayfalar 172 - 179
• Barreto, R. S., Marques, E. J., Jr. Gondim, M. G. C. Vargas de Oliveira, J. (2004), Selection of Beauveria bassiana (Bals.) Vuill. and Metarhizium anisopliae (Metsch.) Sorok. for the control of the mite Mononychellus tanajoa (Bondar), Sci. Agric. (Piracicaba, Braz.), 61 pp. 659–664
• De Hoog, G. (1972). The genera Beauveria, isaria, Tritirachium and Acrodontium gen. nov. Studies in Mycology 1, 1-41.
• Van der Geest LPS, Elliot SL, Breeuwer JAJ, Beerling EAM (2000) Diseases of mites. Exp Appl Acarol 24:497–560
• Humber RA, de Moraes GJ, Santos dos JM (1981) Natural infection of Tetranychus evansi (Acarina: Tetranychidae) by a Triplosporium spp. (Zygomycetes: Entomophthorales) in Northeastern Brazil. Entomophaga 26:421–425
• Irigaray FJSC, Marco-Mancebon V, Perez-Moreno I (2003) The entomopathogenic fungus Beauveria bassiana and its compatibility with triXumuron: eVects on the two-spotted spider mite Tetranychus urticae. Biol Control 26:168–173
• Jackson, M.A. & Jaronskı, S.T. 2009. Production of microsclerotia of the fungal entomopathogen Metarhizium anisopliae and their potential for use as a biocontrol agent for soil-inhabiting insects. Mycological Research 113: 842-850. Kaya, G. P., Hassan, S. 2000. Entomogeneus fungi as promising biopesticides for tick control’, Experimental and Applied Acarology, 24: 913-926.
• Lekimme, M. Mignon, B. Tombeux, S. Focant, C. Maréchal, F. Losson, B. (2006). In vitro entomopathoge- nic activity of Beauveria bassiana against Psoroptes spp. (Acari: Psoroptidae), Veteri- nary Parasitology, 139 pp. 196–202 LIU, Z.Y., Z.Q. LIANG, A.J.S. WHALLEY, Y.-. YAO, AND A.Y. LIU. 2001. Cordyceps brittlebankisoides, a New Pathogen of Grubs and Its Anamorph, Metarhizium anisopliae var. majus. Journal of Invertebrate Pathology 78: 178-182.
• Maniania, N. K., Ekesi, S., Kungu, M. M., Salifu, D. and Srinivasan, R. 2016. The effect of combined application of the entomopathogenic fungus Metarhizium anisopliae and the release of predatory mite Phytoseiulus longipes for the control of the spider mite Tetranychus evansi on tomato. Crop Protect. 90: 49-5).
• Meikle, W. G Mercadier, G. Girod, V.. Derouané, F. Jones, W. A., (2006). Evaluation of Beauveria bassiana (Balsamo) Vuillemin (Deuteromycota: Hyphomycetes) strains isolated from varroa mites in southern France’, Journal of Apicultural Research, 45 pp. 219–220
• Morris, H. and D. F. Waterhouse 2001. The Distribution and Importance of Arthropod Pests and Weeds of Agriculture in Myanmar. ACIAR Monograph 67, ACIAR (Australian Centre for International Agricultural Research) Canberra, Australia
• Ocak I., S. Doğan, N. Ayyıldız & I. Hasenekoğlu 2007. Akarlardan izole edilmiş entomopatojen bir fungus türü: Beauveria bassiana (Balsamo). Journal of the Society for Art and Science, 7: 125-132.
• Rossi-Zalaf, L. and Alves S. B., 2006. Susceptibility of Brevipalpus phoenicis to entomopathogenic fungi. Experimental and Applied Acarology 40(1):37-47
• Sáenz-de-Cabezón Irigaray, F. J. Marco–Mancebón, V. Pérez-Moreno, I., (2003). The entomopathogenic fun- gus Beauveria bassiana and compatibility with triflumeron: effects on the twospotted spi- der mite Tetranychus urticae’, Biological Control, 26 pp. 168–173
• Samson RA, Evans HC, Latgé J-P. 1988.Atlas of entomopathogenic fungi. Berlin: Springer. Shaw, K. E. Davidson, G. Clark, S. J.. Ball, B. V. Pell, J. K Chandler, D. Sunderland, K. D. (2002). Labora- tory bioassays to assess the pathogenicity of mitosporic fungi to Varroa destructor (Aca- ri: Mesostigmata), an ectoparasitic mite of the honeybee, Apis mellifera’, Biological Cont rol, 24 pp. 266–276
• Shi, W. B. Feng, M. G. (2004). Lethal effect of Beauveria bassiana, Metarhizium anisopliae, and Paeci- lomyces fumosoroseus on the eggs of Tetranychus cinnabarinus (Acari: Tetranychidae) with a description of a mite egg bioassay system’, Biological Control, 30 pp. 165–173
• Tamai MA, Alves SB, Almeida JEM, Faion M. 2002. Avaliacão de fungos entomopatogêncos para o controle de Tetranychus urticae (Acari: Tetranychidae). Arquivos do Instituto Biológico 64: 77–84.
• van der Geest LPS, Elliot SL, Breeuwer JAJ, Beerling EAM (2000) Diseases of mites. Exp Appl Acarol 24:497–560
• Wekesa VW, Maniania NK, Knapp M, Boga HI (2005) Pathogenicity of Beauveria bassiana and Metarhizium anisopliae to the tobacco spider mite Tetranychus evansi. Exp Appl Acarol 36:41–50