Dopamine Detection From PC12 Cells With A Carbon-fiber Microelectrode Controlled By A Hommade System

Yıl 2023, Sayı: 52, 104 - 109, 15.12.2023

Tuğba Akkaş , Mustafa Şen

Öz

Dopamine (DA) is a neurotransmitter that is naturally produced in the body. Dopamine provides nerve conduction in the central nervous system and plays an important role in the regulation and control of movements, motivation and cognitive development.. Imbalance in the amount of DA can cause many diseases ranging from Parkinson's disease to depression. In this study, PC12 cells were chemically stimulated with potassium ions and local electrochemical measurement of DA released by the cells was performed.First, the production of needle-tipped CFMDE was carried out by micropulling method. Carbon-fiber microdisk electrodes are frequently used for effective and high sensitivity detection of dopamine. Afterwards, cell clusters of PC12 cells were obtained and the change in dopamine amount in PC12 cell clusters was measurement. For the measurement process, a microcontroller unit integrated to a inverted microscope was used to precisely position the needle-tipped electrodes. DA release was successfully measured as a result of chemical stimulation with K+ ions. Basically, a homemade system was developed to analyze DA release triggered by chemical or physical stimuli.

Anahtar Kelimeler

Carbon-fiber Microelectrode, Dopamine, PC12 cells, Electrochemical Detection, Local Analysis

Destekleyen Kurum

TUBITAK

Proje Numarası

1919B012218283

Teşekkür

We thank Dr. Özkur KURAN for his help in the installation of the microcontroller unit.

Ev Yapımı Bir Sistem Tarafından Kontrol Edilen Karbon Fiber Mikroelektrot ile PC12 Hücrelerinden Dopamin Tespiti

Öz

Dopamin (DA), vücutta doğal olarak üretilen bir nörotransmiterdir. Dopamin, merkezi sinir sisteminde sinir iletimini sağlar ve hareketlerin düzenlenmesi ve kontrolünde, motivasyonda ve bilişsel gelişimde önemli rol oynar. DA miktarındaki dengesizlik, Parkinson hastalığından depresyona kadar birçok hastalığa neden olabilir. Bu çalışmada PC12 hücreleri potasyum iyonları ile kimyasal olarak uyarıldı ve hücreler tarafından salınan DA'nın lokal elektrokimyasal ölçümü gerçekleştirildi. İlk olarak mikro çekme yöntemi ile iğne uçlu karbon fiber mikroelektrotların üretimi gerçekleştirildi. Karbon fiber elektrotlar, dopaminin etkili ve yüksek hassasiyetli tespiti için sıklıkla kullanılır. Sonrasında PC12 hücrelerinin hücre kümeleri elde edildi ve Pc12 hücre kümelerindeki dopamin miktarındaki değişim ölçüldü. Ölçüm işlemi için, iğne uçlu elektrotları hassas bir şekilde konumlandırmak için ters bir mikroskoba entegre edilmiş bir mikro denetleyici ünitesi kullanıldı. DA salınımı, K+ iyonları ile kimyasal stimülasyonun bir sonucu olarak başarıyla ölçüldü. Temel olarak, kimyasal veya fiziksel uyaranlarla tetiklenen DA salınımını analiz etmek için ev yapımı bir sistem geliştirildi.

Anahtar Kelimeler

Karbon fiber Mikroelektrot, Dopamin, PC12 hücreleri, Elektrokimyasal Tespit, Lokal Analiz

Proje Numarası

1919B012218283

Kaynakça

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