Determination of Antioxidant Activity of Dietary Selenium, Oleuropein, Glutathione Mixture

Abstract

Free radicals area unit involved with cellular disorders through their damaging actions on proteins, lipids and DNA and area unit causative factors for an outsized variety of chronic diseases and therefore the aging method. Antioxidants of plant origin hold nice significance and have so gained utmost importance within the recent past. The current research spills inhibition effect of selenium, glutathione and oleuropein mixtures against free radicals and supermolecule chemical action. It was determined that the mixture of oleuropein, glutathione and selenium used in the study inhibited free radicals. It was determined that it competed with the positive control at increasing concentrations. The mixture is intended to be used as a daily support supplement. The aim of the study is to use the prepared mixture as an alternative to drugs known to have artificial antioxidant properties in the market. One of the aims of this study is to have both drug therapy in the literature and inhibition of ROS-induced damage in the biological system at an early stage.

Keywords

• free radicals
• gluthatione
• selenium
• oleuropein
• ROS(reactive oxygen specie

References

1. [1] Pietta PG. Flavonoids as antioxidants. Journal of natural products, 63(2000) 1035–1042. https://doi.org/10.1021/np9904509
2. [2] Tokusoglu, O. (2016). Effect of high hydrostatic pressure processing strategies on retention of antioxidant phenolic bioactives in foods and beverages–a review. Polish Journal of Food and Nutrition Sciences, 66(4).
3. [3] Leopoldini, M., Marino, T., Russo, N., & Toscano, M. (2004). Antioxidant properties of phenolic compounds: H-atom versus electron transfer mechanism. The Journal of Physical Chemistry A, 108(22), 4916-4922. 2
4. [4] Bonnefont-Rousselot D, Thérond P, Beaudeux JL, Peynet J, Legrand A, Delattre J. High density lipoproteins (HDL) and the oxidative hypothesis of atherosclerosis. Clin Chem Lab Med. 37(1999) 939-948 doi:10.1515/CCLM.1999.139
5. [5] Pillai, S. S., Sugathan, J. K., & Indira, M. (2012). Selenium downregulates RAGE and NFκB expression in diabetic rats. Biological trace element research, 149, 71-77.
6. [6] Hwang, J. Y., Zhang, J., Kang, M. J., Lee, S. K., Kim, H. A., Kim, J. J., & Kim, J. I. (2007). Hypoglycemic and hypolipidemic effects of Saururus chinensis Baill in streptozotocin-induced diabetic rats. Nutrition Research and Practice, 1(2), 100-104.
7. [7] Ayaz, M., Ozdemir, S., Ugur, M., Vassort, G., & Turan, B. (2004). Effects of selenium on altered mechanical and electrical cardiac activities of diabetic rat. Archives of Biochemistry and Biophysics, 426(1), 83-90.
8. [8] Liu, H., Wang, H., Shenvi, S., Hagen, T. M., & LIU, R. M. (2004). Glutathione metabolism during aging and in Alzheimer disease. Annals of the New York Academy of Sciences, 1019(1), 346-349

9. [9] Hassen, I., Casabianca, H., & Hosni, K. (2015). Biological activities of the natural antioxidant oleuropein: Exceeding the expectation–A mini-review. Journal of Functional Foods, 18, 926-940.
10. [10] Ahamad, J., Toufeeq, I., Khan, M. A., Ameen, M. S. M., Anwer, E. T., Uthirapathy, S., ... & Ahmad, J. (2019). Oleuropein: A natural antioxidant molecule in the treatment of metabolic syndrome. Phytotherapy Research, 33(12), 3112-3128.
11. [11] Khalatbary, A. R., & Ahmadvand, H. (2012). Neuroprotective effect of oleuropein following spinal cord injury in rats. Neurological research, 34(1), 44-51.
12. [12] Gülçin I, Küfrevioglu OI, Oktay M, Büyükokuroglu ME, Antioxidant, antimicrobial, antiulcer and analgesic activities of nettle (Urtica dioica L.). Journal of ethnopharmacology, 90(2004) 205–215. https://doi.org/10.1016/j.jep.2003.09.028
13. [13] Wang L, Yen JH, Liang HL and Wu MJ. "Antioxidant effect of methanol extracts from lotus plumule and blossom (Nelumbo nucifera Gertn.)," Journal of Food and Drug Analysis 11 (2003) https://doi.org/10.38212/2224-6614.2726.
14. [14] Shimada K, Fujikawa K, Yahara K, Nakamura T, Antioxidative properties of xanthan on the autoxidation of soybean oil in cyclodextrin emulsion. Journal of agricultural and food chemistry, 40(1992) 945-948.
15. [15] Mazumder A, Gerlt JA, Absalon MJ., Stubbe J, Cunningham RP, Withka J, Bolton PH, Stereochemical studies of the. Beta.-elimination reactions at aldehydic abasic sites in DNA: endonuclease III from Escherichia Coli, sodium hydroxide, and Lys-Trp-Lys. Biochemistry, 30(1991) 1119-1126.