Heterorhabditis bacteriophora Poinar, 1976 (Rhabditida: Heterorhabditidae) HBH hibrit ırkının in vitro katı kültürde üretiminin optimizasyonu

Öz

Entomopatojen nematodlar (EPN), toprakta yaşayan biyolojik mücadele ajanlarıdır. EPN'ler yaşam döngüleri boyunca bir konukçu böceğe ihtiyaç duyarlar ve gelişimleri sırasında konukçusunu öldürürler. EPN'lerin en büyük dezavantajı, ticari ürünlerin yüksek maliyetidir. Bu nedenle kitle üretimin optimizasyonu ile üretim maliyetlerinin düşürülmesine odaklanan birçok çalışma bulunmaktadır. Daha önceki bir proje kapsamında iki yerel izolattan Heterorhabditis bacteriophora Poinar, 1976 (Rhabditida: Heterorhabditidae) HBH hibrit ırkı geliştirilmiştir. Bu hibrit ırk, üstün biyo-ekolojik özelliklerinden dolayı patentlidir. Bu çalışmada, hibrit HBH ırkının in vitro katı kültürde kitle üretiminin optimize edilmesi amaçlanmıştır. Tüm laboratuvar denemeleri 2017-2018 yılları arasında Bursa Uludağ Üniversitesi, Ziraat Fakültesi, Bitki Koruma Bölümü, Nematoloji Laboratuvarı'nda gerçekleştirilmiştir. Optimizasyonda standart ortama ek katkı maddeleri (soya lesitini ve yumurta sarısı), sıcaklık (24, 28 ve 32°C) ve ortam pH'ı (5, 7 ve 9) üretim parametreleri olarak seçilmiştir. Optimizasyon, hermafrodit yumurta sayıları, toplam infektif jüvenil (IJ) sayısı, IJ vücut uzunluğu ve IJ etkinliği ile değerlendirilmiştir. Sonuçlara göre, en iyi üretim kombinasyonu, soya lesitini içeren agar, 28°C ve pH 7 olarak bulunmuştur. Ayrıca pH 9'lu agarlar, üretim verimini önemli ölçüde azaltmıştır. Sonuç olarak, HBH hibrit ırkının bazı önemli in vitro katı üretim parametreleri için optimum değerler belirlenmiştir.

Anahtar Kelimeler

• Heterorhabditis bacteriophora
• kitle üretimi
• monoksenik kültür
• optimizasyon

Optimization of in vitro solid culture of Heterorhabditis bacteriophora Poinar, 1976 (Rhabditida: Heterorhabditidae) HBH hybrid strain

Abstract

Entomopathogenic nematodes are soil-dwelling biocontrol agents. EPNs need an insect host to complete their life cycle, and they kill their host during its development. The major disadvantage of EPNs is the high cost of commercial products. Thus, there are many studies focused on reducing production costs by optimization of mass production. In a previous project, Heterorhabditis bacteriophora Poinar, 1976 (Rhabditida: Heterorhabditidae) HBH hybrid strain was developed from local isolates. This hybrid strain was patented due to superior bioecological characteristics. This study aimed to optimize in vitro solid mass production of hybrid strain. All laboratory trials were performed between 2017 and 2018, in Nematology Laboratory of Bursa Uludağ University, Faculty of Agriculture, Department of Plant Protection. For optimization, additional supplementary ingredients (soy lecithin and egg yolk), temperature (24, 28 and 32°C) and medium pH (5, 7 and 9) were selected as production parameters. Optimization was evaluated based on hermaphrodite egg numbers, total infective juveniles (IJs), IJ body length and IJ virulence. Based on results, best production combination was found as agar containing soy lecithin, 28°C and pH 7. Also, agar media with pH 9 markedly reduced production yield. Consequently, optimum values for some important in vitro solid production parameters of HBH hybrid strain were determined.

Keywords

• Heterorhabditis bacteriophora
• mass production
• monoxenic culture
• optimization

Kaynakça

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