Dolerus Panzer, 1801 (Hymenoptera: Tenthredinidae) cinsinde ITS2'nin karakterizasyonu ve taksonomik kullanımı

Öz

Böceklerde ITS2'nin moleküler belirteç olarak kullanılmasının yaygınlaşması, tür düzeyindeki filogeni çalışmalarına hız kazandırmıştır. Bu belirtecin hem hızlı evrime sahip olması hem de ikincil yapı oluşturması gibi özellikler sayesinde güvenilir ve kaliteli veriler elde edilebilir. Bu makalede, Dolerus Panzer, 1801 cinsine ait 2002-2018 yılları arasında toplanmış olan 36 bireyin (Hymenoptera: Tenthredinidae) aralarındaki filogenetik ilişkiyi ve ITS2 ikincil yapı özelliklerine ilişkin ilk verileri sunuyoruz. ITS2'nin hizalanmış verileri, örnekler arasındaki ilişkiyi görmek için maksimum likelihood yöntemi uygulanarak analiz edildi. Aynı zamanda ITS2'nin yapısal özellikleri, uzunluk polimorfizmi ve CBC'lerin olması, yakından ilişkili türlerin tür sınırlarının belirlenmesinde faydalıdır, Dolerus cinsine ait 36 bireyden, 4 tür (Dolerus triplicatus (Klug, 1818), Dolerus germanicus (Fabricus, 1775), Dolerus puncticollis Thomson, 1871 ve Dolerus nigratus (Müller, 1776) ve 2 olası tür (Dolerus spp. 1 ve 2) belirlenmiştir.

Anahtar Kelimeler

• CBC
• Dolerus
• Hymenoptera
• ITS2
• moleküler belirteç

Characterization and taxonomic utility of ITS2 in Dolerus Panzer, 1801 (Hymenoptera: Tenthredinidae)

Abstract

The widespread use of ITS2 as a potential marker in insects has accelerated species-level phylogenetic studies. Reliable and quality data can be obtained thanks to the features such as rapid evolution and secondary structure of this marker. This paper presents the phylogenetic relationship among 36 individuals of Dolerus Panzer, 1801 (Hymenoptera: Tenthredinidae) collected between 2002 and 2018 to obtain the first data on ITS2 secondary structure. Aligned of ITS2 data were analyzed by application of maximum likelihood method to reveal phylogenetic relationship among the specimens. Also, the structural properties, length variation and presence of compensatory base changes make the ITS2 useful marker in determination of species boundaries of closely related species. Four species (Dolerus triplicatus (Klug, 1818), Dolerus germanicus (Fabricius, 1775), Dolerus puncticollis Thomson, 1871 and Dolerus nigratus (Müller, 1776) and two putative species (Dolerus spp. 1 and 2) were determined from 36 individuals belonging to Dolerus.

Keywords

• Compensatory base changes
• Dolerus
• Hymenoptera
• ITS2
• molecular marker

Kaynakça

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