Ditylenchus dipsaci (Kühn, 1857) (Tylenchida: Anguinidae)’ye karşı dayanıklı Türk nohut genotiplerini belirlemek için in vivo biyolojik testlerin geliştirilmesi

Öz

Soğan sak nematodu, Ditylenchus dipsaci (Kühn, 1857) (Tylenchida: Anguinidae) en önemli zarar yapan bitki paraziti nematodlarından biridir ve nohut gibi birçok baklagillere zarar vermektedir. Çalışmanın amacı, D. dipsaci' nin nohut ile etkileşimini araştırmak için bir in-vivo biyolojik test protokolü geliştirmektir. Ditylenchus dipsaci'ye dayanıklılık açısından nohut genotipini değerlendirmek için üçü Cicer reticulatum Ladiz. (Fabales: Fabaceae), üçü Cicer echinospermum P. H. Davis (Fabales: Fabaceae) ve üçü Cicer arietinum L. (Fabales: Fabaceae)] dahil olmak üzere yabani ve yerli Cicer türlerinin dokuz genotipi 2019-2020 yılları arasında Çukurova Üniversitesi Nematoloji laboratuvarında test edilmiştir. İnkübasyon zamanı ve inokulum yoğunluğunun, üretilen nematod popülasyonu miktarıyla bağlantılı olduğu belirlenmiştir. Ditylenchus dipsaci'nin havuç disklerinde en yüksek üremesinin, 20°C ile 25°C arasında 45-60 günde olduğu ve başlangıç inokulum yoğunluğunun havuç disk başına 100 nematod olarak saptanmıştır. Ayrıca, 300 adet bireyden oluşan inokulum yoğunluğu ve 16 hafta inkübasyon süresi nohut genotiplerinde D. dipsaci ’ye dayanıklılık için en iyi uygulama olmuştur. Bu yöntemler D. dipsaci’ye karşı dayanıklılık çalışmalarında hızlı testleme yapılması için; D. dipsaci en yüksek üreme periyodunun zaman, sıcaklık ve inokulum yoğunluğu olarak gözlenmesini sağlamıştır.

Anahtar Kelimeler

• Havuç kültürü
• nohut
• Ditylenchus dipsaci
• inokulum yoğunluğu
• testleme yöntemleri

Development of an in vivo bioassay to identify Turkish chickpea genotypes resistance to Ditylenchus dipsaci (Kühn, 1857) (Tylenchida: Anguinidae)

Öz

Stem and bulb nematode, Ditylenchus dipsaci (Kühn, 1857) (Tylenchida: Anguinidae) is one of the most damaging plant-parasitic nematodes and damage most legume crops such as chickpea. The aim of the study was to develop a protocol for in-vivo bioassay to investigate D. dipsaci interaction with chickpea. Nine accessions of wild and domesticated Cicer spp. including three Cicer reticulatum Ladiz., three Cicer echinospermum P.H.Davis and three Cicer arietinum L. (Fabales: Fabaceae) were used to evaluate chickpea genotype for resistance to D. dipsaci between 2019 and 2020 at Çukurova University, Nematology laboratory. The incubation time and inoculum density were determined to correlate with the size of the produced nematode population. It was concluded that the highest reproduction of D. dipsaci on carrot discs occurred between 20 and 25°C at 45-60 days and an initial inoculum density were determined 100 nematodes per carrot disc. Also, the initial inoculum density of 300 and the growing time of 16 weeks was the best practice to identify reaction of chickpea genotypes to D. dipsaci. These methods provided that quick screening for resistance studies in chickpea against D. dipsaci to observe information of highest reproduction period of D. dipsaci as time, temperature, inoculum density.

Anahtar Kelimeler

• Carrot culture
• chickpea
• Ditylenchus dipsaci
• inoculum density
• screening methods

Kaynakça

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