Tokat İli (Türkiye) orman alanlarından izole edilen yerel entomopatojen fungusların patates böceği Leptinotarsa decemlineata (Say, 1824) (Coleoptera: Chrysomelidae)’ya etkisi

Öz

Tokat İli (Türkiye) ve 11 ilçesinden 2014-2017 yılları arasında toplanan orman toprak örneklerinden izole edilen entomopatojenik fungusların, Kolorado patates böceği, Leptinotarsa decemlineata (Say, 1824) (Coleoptera: Chrysomelidae)'nın üçüncü dönem larva ve erginleri üzerindeki etkileri laboratuvar koşullarında değerlendirilmiştir. İlk olarak 33 izolatın L. decemlineata larvaları ve erginleri üzerindeki etkinliğini belirlemek için 1×108 konidi/ml’de tek doz etkinlik denemeleri yapılmıştır. Tek doz etkinlik denemelerinde en yüksek ölüm oranlarını veren dört izolat olan Beauveria bassiana (Balsamo-Crivelli) Vuillemin (Hypocreales: Cordycipitaceae)’yı içeren GOPT-498-4, GOPT-529-2, GOPT-552, GOPT-562 izolatları 1×103, 1×105, 1×107, 1×108, 1×109 konidi/ml’de doz-ölüm denemelerinde kullanılmıştır. En düşük LC50 ve LT50 değerleri sırasıyla 1.4×106 konidi/ml ve 10.6 gün ile GOPT-552 izolatı için kaydedilmiş olup, bunu GOPT-562 ve GOPT-529-2 izolatları takip etmiştir. Beauveria bassiana (GOPT-552, GOPT-562) izolatları L. decemlineata larva ve erginlerine karşı daha etkili olmuştur. Dolayısıyla GOPT-552 ve GOPT-562 izolatlarının Kolorado patates böceğinin biyolojik kontrolü için potansiyele sahip olduğu görülmüştür.

Anahtar Kelimeler

• Beauveria bassiana
• mikrobiyal mücadele
• mikopestisit
• zararlılar
• virulans

Efficacy of local entomopathogenic fungi isolated from forestlands in Tokat Province (Türkiye) against the Colorado potato beetle, Leptinotarsa decemlineata (Say, 1824) (Coleoptera: Chrysomelidae)

Abstract

Effects of the entomopathogenic fungi, isolated from forest soil samples gathered from Tokat Province (Türkiye) and its 11 districts between 2014-2017, were evaluated on the third instars and adults of the Colorado potato beetle, Leptinotarsa decemlineata (Say, 1824) (Coleoptera: Chrysomelidae) under laboratory conditions. Initially, single-dose efficacy experiments were conducted to determine the effect of the 33 isolates on L. decemlineata larvae and adults at 1×108 conidia/ml. The four isolates giving the highest mortality in single-dose efficacy experiments, GOPT-498-4, GOPT-529-2, GOPT-552, GOPT-562 that included Beauveria bassiana (Balsamo-Crivelli) Vuillemin (Hypocreales: Cordycipitaceae), were used in dose-mortality experiments at 1×103, 1×105, 1×107, 1×108, 1×109 conidia/ml. The lowest LC50 and LT50 values were recorded for GOPT-552 with 1.4×106 conidia/ml and 10.6 days, respectively, followed by GOPT-562 and GOPT-529-2. Beauveria bassiana (GOPT-552 and GOPT-562) isolates were more effective against L. decemlineata larvae and adults. Accordingly, GOPT-552 and GOPT-562 isolates are considered to have potential for biological control of Colorado potato beetle.

Keywords

• Beauveria bassiana
• microbial control
• mycopesticide
• pests
• virulence

Kaynakça

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