Antalya (Türkiye)’dan Bemisia tabaci (Genn., 1889) (Hemiptera: Aleyrodidae) popülasyonlarının cyantraniliprole, pyriproxyfen ve spirotetramata direnç düzeyleri

Öz

Çalışmada, Antalya İli (Türkiye)’nden 2019 ve 2020 yıllarında toplanan Bemisia tabaci (Genn., 1889) (Hemiptera: Aleyrodidae)’nin oniki farklı MEAM1 ve MED popülasyonlarının spirotetramat, pyriproxyfen ve cyantraniliprole karşı duyarlılık düzeyleri belirlenmiştir. Popülasyonların lethal konsantrasyon (LC50) değerleri spirotetramat ve pyriproxyfen için yaprak daldırma yöntemi ile sırasıyla 2. larva ve yumurta dönemine uygulanarak ve cyantraniliprole için sistemik alım yöntemi ile larva dönemine uygulanarak belirlenmiştir. Çalışmada popülasyonların LC50 değerlerinin duyarlı popülasyonunun LC50 değerine bölünmesiyle popülasyonların direnç katları belirlenmiştir. Popülasyonların LC50 değerleri, pyriproxyfen için 0.28 ila 1.70x103 mg a.i./l, spirotetramat için 1.76 ila 228 mg a.i./l ve cyantraniliprole için 0.103 ila 0.382 mg a.i./l aralıklarında belirlenmiştir. Popülasyonların pyriproxyfen için direnç katları çok yüksek seviyede tespit edilmiştir. Direnç oranlarının spirotetramat için 2.38 ile 309 kat arasında ve cyantraniliprole için 4.68 ile 17.4 kat arasında değiştiği tespit edilmiştir. Sonuçlara göre, tüm popülasyonların cyantraniliprole karşı duyarlı olduğu tespit edilirken, pyriproxyfen ve spiroteramata karşı bazı popülasyonlarda yüksek düzeyde direnç tespit edilmiştir. Sonuçlar, insektisit direncinin gelecekte izlenmesi ve yönetimi için referans verileri içermektedir.

Anahtar Kelimeler

• Biyotip
• insektisit
• direnç
• hassasiyet
• beyazsinek

Insecticide resistance status of Bemisia tabaci (Genn., 1889) (Hemiptera: Aleyrodidae) populations to cyantraniliprole, pyriproxyfen and spirotetramat in Antalya (Türkiye)

Abstract

In the study, the susceptibility of twelve Bemisia tabaci (Genn., 1889) (Hemiptera: Aleyrodidae) MEAM1 and MED populations collected from tomato and pepper greenhouses in Antalya Province (Türkiye) in 2019 and 2020 to spirotetramat, pyriproxyfen and cyantraniliprole were determined. To determine the lethal concentrations (LC50) for the populations, spirotetramat and pyriproxyfen were applied using leaf dipping method to second instar and eggs, respectively, while a systemic uptake method was used for testing the susceptibility of whitefly instars to cyantraniliprole. The resistance ratios were calculated by dividing the LC50 of the populations by the LC50 of a susceptible population. The LC50 of the populations ranged from 0.28 to 1.70x103 mg a.i./l for pyriproxyfen, from 1.76 to 228 mg a.i./l for spirotetramat, and from 0.103 to 0.382 mg a.i./l for cyantraniliprole. Resistance ratios for pyriproxyfen were particularly high. For spirotetramat and cyantraniliprole resistance varied between 2.38 and 309, and 4.68 to 17.4 times, respectively. All populations were susceptible to cyantraniliprole, but some populations highly resistance to pyriproxyfen and spirotetramat. The results will be a valuable reference for future monitoring and management of insecticide resistance.

Keywords

• Biotype
• insecticide
• resistance
• susceptibility
• whitefly

Kaynakça

1. Abdullahi, I., S. Winter, G. I. Atiri & G. Thottappilly, 2003. Molecular characterization of whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae) populations infesting cassava. Bulletin of Entomological Research, 93 (2): 97-106.
2. Anonymous, 2021. Republic of Turkey Ministry of Agriculture and Forestry General Directorate of Food and Control. (https://www.tarimorman.gov.tr/GKGM/Belgeler/DB_Bitki_Koruma_Urunleri/Istatistik/Il_Duzeyinde_BKU_Kullanim_Miktar_2018.pdf) (Date accessed November 2021) (in Turkish).
3. APRD, 2018. Arthropod Pesticide Resistance Management Bemisia tabaci. (Web page: https://www.pesticideresistance.org/) (Date accessed: November 2018).
4. Bahşi, Ş. Ü., F. Dağlı, C. İkten & H. Göçmen, 2012. Susceptibility level of Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) populations collected from Antalya to Acetamiprid, Chlorpyrifos-ethyl and Cypermethrin. Akdeniz Üniversitesi Ziraat Fakültesi Dergisi 25 (1): 17-22 (in Turkish with abstract in English).
5. Bielza, P., I. Moreno, A. Belando, C. Grávalos, J. Izquierdo & R. Nauen, 2019. Spiromesifen and spirotetramat resistance in field populations of Bemisia tabaci Gennadius in Spain. Pest Management Science 75 (1): 45-49.
6. Bielza, P., V. Quinto, J. Contreras, M. Torne, A. Martin & P. J. Espinosa, 2007. Resistance to spinosad in the western flower thrips, Frankliniella occidentalis (Pergande), in greenhouses of south-eastern Spain. Pest Management Science, 63: 682-687.
7. Bretschneider, T., J. Benetbuchhol, R. Fischer & R. Nauen, 2003. Spirodiclofen and spiromesifen novel acaricidal and insecticidal tetronic acid derivatives with a new mode of action. Chimia, 57 (11): 697-701.
8. Caballero, R., S. Cyman, D. J. Schuster, H. E. Portillo & R. Slater, 2013. Baseline susceptibility of Bemisia tabaci (Genn.) biotype B in southern Florida to cyantraniliprole. Crop Protection, 44: 104-108.

9. Dağlı, F., U. Yükselbaba, C. Ikten, N. Topakçı & H. Göçmen, 2020a. Molecular identification of cotton whitefly Bemisia tabaci Genn. (Hemiptera: Aleyrodidae) populations of Turkey based on mitochondrial cytochrome oxidase subunit I. Applied Ecology and Environmental Research 18 (2): 2351-2361.
10. Dağlı, F., U. Yükselbaba, C. Ikten, N. Topakçı & H. Göçmen, 2020b. Insecticide resistance in Bemisia tabaci (Genn.) Populations collected from the Mediterranean and Aegean regions of Turkey. Applied Ecology & Environmental Research, 18 (6): 7757-7768.
11. De Barro, P. J., F. Driver, J. W. H. Trueman & J. Curran, 2000. Phylogenetic relationships of world populations of Bemisia tabaci (Gennadius) using ribosomal ITS1. Molecular Phylogenetics and Evolution, 16 (1): 29-36.
12. De la Rua, P., B. Simon, D. Cifuentes, C. Martinez-Mora & J. L. Cenis, 2006. New insights into the mitochondrial phylogeny of the whitefly Bemisia tabaci (Hemiptera: Aleyrodidae) in the Mediterranean Basin. Journal of Zoological Systematics and Evolutionary Research, 44 (1): 25-33.
13. Denholm, I., M. Cahill, F. J. Byrne & A. L. Devonshire, 1996. “Progress with Documenting and Combating Insecticide Resistance in Bemisia, 507-603”. In: Bemisia: 1995, Taxonomy, Biology, Damage, Control and Management (Eds. D. Gerling & R. T. Mayer). Intercept Ltd Andover, Hants, 702 pp.
14. El Kady, H. & J. G. Devine, 2003. Insecticide resistance in Egyptian populations of the cotton whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae). Pest Management Science, 59 (8): 865-871.
15. Elbert, A. & R. Nauen, 2000. Resistance of Bemisia tabaci (Homoptera: Aleyrodidae) to insecticides in southern Spain with special reference to neonicotinoids. Pest Management Science, 56 (1): 60-64.
16. Erdoğan, C., G. D. Moores, M. O. Gurkan, K. J. Gorman & I. Denholm, 2008. Insecticide resistance and biotype status of populations of the tobacco whitefly Bemisia tabaci (Hemiptera: Aleyrodidae) from Turkey. Crop Protection, 27 (3-5): 600-605.
17. Fernandez, E., C. Grávalos, P. J. Haro, D. Cifuentes & P. Bielza, 2009. Insecticide resistance status of Bemisia tabaci Q-biotype in south-eastern Spain. Pest Management Science; 65 (8): 885-891.
18. Frohlich, D. R., I. Torrez-jerez, I. D. Bedford, P. G. Markham & J. K. Brown, 1999. A phylogeographical analysis of the Bemisia tabaci species complex based on Mitochondrial DNA markers. Molecular Ecology, 8 (10): 1683-1691.
19. Gravalos, C., E. Fernández, A. Belando, I. Moreno, C. Rosb & P. Bielza, 2015. Cross-resistance and baseline susceptibility of Mediterranean strains of Bemisia tabaci to cyantraniliprole. Pest Management Science, 71 (7): 1030-1036.
20. Hopkinson, J. E. & S. E. Pumpa, 2019. Baseline susceptibility of Bemisia tabaci MEAM 1 (Hemiptera: Aleyrodidae) in Australia to spiroteramat, cyantraniliprole and dinotefuran, with reference to pyriproxyfen cross-resistance. Austral Entomology, 58 (4): 762-771.
21. Hopkinson, J., S. Pumpa, S. Van Brunschot, C. Fang, M. Frese, W. T. Tay & T. Walsh, 2020. Insecticide resistance status of Bemisia tabaci MEAM1 (Hemiptera: Aleyrodidae) in Australian cotton production valleys. Australian Entomology, 59 (1): 202-214.
22. Horowitz, A. R., I. Denholm, K. Gorman, J. L. Cenis, S. Kontsedalov & I. Ishaaya, 2003. Biotype Q of Bemisia tabaci identified in Israel. Phytoparasitica, 31 (1): 94-98.
23. Horowitz, A. R. & I. Ishaaya, 2014. Dynamics of biotypes B and Q of the whitefly Bemisia tabaci and its impact on insecticide resistance. Pest Management Science, 70 (10): 1568-1572.
24. Horowitz, A. R., S. Kontsedalov, I. Denholm & I. Ishaaya, 2002. Dynamics of insecticide resistance in Bemisia tabaci: a case study with the insect growth regulator pyriproxyfen Pest Management Science, 58 (11): 1096-1100.
25. Horowitz, A. R., S. Kontsedalov, V. Khasdan & I. Ishaaya, 2005. Biotypes B and Q of Bemisia tabaci and their relevance to neonicotinoid and pyriproxyfen resistance. Archives of Insect Biochemistry and Physiology, 58 (4): 216-225.
26. Horowitz, A. R., Z. Mendelson, M. Cahill, I. Denholm & I. Ishaaya, 1999. Managing resistance to the insect growth regulator, pyriproxyfen, in Bemisia tabaci. Pest Management Science, 55 (3): 272-276.
27. Horowitz, A. R., Z. Mendelson, P. G. Weintraub & I. Ishaaya, 1998. Comparative toxicity of foliar and systemic applications of acetamiprid and imidacloprid against the cotton whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae). Bulletin of Entomological Research, 88 (4): 437-442.
28. IRAC (Insecticide Resistance Action Committee), 2019. Susceptibility test methods no: 016. (Web page: https://www.irac-online.org/methods) (Date accessed: July 2019).
29. Karut, K., M. M. Karaca, İ. Döker & C. Kazak, 2017. Analysis of species, subgroups, and endosymbionts of Bemisia tabaci (Hemiptera: Aleyrodidae) from southwestern cotton fields in Turkey. Environmental Entomology, 46 (4) 1035-1040.
30. Khasdan, V., I. Levin, A. Rosner, S. Morin, S. Kontsedalov, L. Maslenin & A. R. Horowitz, 2005. DNA markers for identifying biotypes B and Q of Bemisia tabaci (Hemiptera: Aleyrodidae) and studying population Dynamics. Bulletin of Entomological Research, 95 (6): 605-613.
31. Kontsedalov, S., F. Abu-Moch, G. Lebedev, H. Czosnek, A. R. Horowitz & M. Ghanim, 2012. Bemisia tabaci biotype dynamics and resistance to insecticides in Israel during the years 2008-2010. Journal of Integrative Agriculture, 11 (2): 312-320.
32. Li, X. C., B. A. Degain, V. S. Harpold, P. G. Marcon, R. L. Nichols, A. J. Fournier, S. E. Naranjo, J. C. Palumbo & P. C. Ellsworth, 2012. Baseline susceptibilities of B and Q biotype Bemisia tabaci to anthranilic diamides in Arizona. Pest Management Science, 68 (1): 83-91.
33. Ma, D., K. Gorman, G. Devine, W. Luo & I. Denholm, 2007. The biotype and insecticide-resistance status of whiteflies, Bemisia tabaci (Hemiptera: Aleyrodidae), invading cropping systems in Xinjiang Uygur Autonomous Region, northwestern China. Crop Protection, 26 (4): 612-617.
34. Ma, W., X. Li, T.J. Dennehy, C. Lei, M. Wang, B. A. Degain & R. L. Nichols, 2010. Pyriproxyfen resistance of Bemisia tabaci (Homoptera: Aleyrodidae) biotype B: metabolic mechanism. Journal of Economic Entomology, 103 (1): 158-165.
35. Martin, J. H., D. Mifsud & C. Rapisarda, 2000. The whiteflies (Hemiptera: Aleyrodidae) of Europe and the Mediterranean basin. Bulletin of Entomological Research, 90 (5): 407-448.
36. Mohammed, M. A., M. M. Karaca, İ. Döker & K. Karut, 2020. Monitoring insecticide resistance and endosymbiont composition, in greenhouse populations of Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) from Mersin, Turkey. Phytoparasitica, 48 (4): 659-672.
37. Nauen, R., H. J. Schnorbach & A. Elbert, 2005. The biological profile of spiromesifen (Oberon) a new tetronic acid insecticide/acaricide. Pflanzenschutz-Nachrichten Bayer, 58: 417-440.
38. Peng, Z., H. Zheng, W. Xie, S. Wang, Q. Wu & Y. Zhang, 2017. Field resistance monitoring of the immature stages of the whitefly Bemisia tabaci to Field resistance monitoring of the immature stages of the whitefly Bemisia tabaci to spirotetramat in China. Crop Protection, 98 (2017): 243-247.
39. Perring, T. M., 2001. The Bemisia tabaci species complex. Crop Protection, 20 (9): 725-737.
40. Prabhaker, N., S. Castle & T. M. Perring, 2014. Baseline susceptibility of Bemisia tabaci B biotype (Hemiptera: Aleyrodidae) populations from California and Arizona to spirotetramat. Journal of Economic Entomology, 107 (2): 773-780.
41. Satar, G., M. R. Ulusoy, R. Nauen & K. Dong, 2018. Neonicotinoid insecticide resistance among populations of Bemisia tabaci in the Mediterranean region of Turkey. Bulletin of Insectology, 71 (2): 171-177.
42. Torres-Vila, L. M., M. C. Rodriguez-Molina, A. Lacasa-Plasencia & P. Bielza-Lino, 2002. Insecticide resistance of Helicoverpa armigera to endosulfan, carbamates and organophosphates: the Spanish case. Crop Protection, 21 (19): 1003-1013.
43. Toscano, N. C., N. Prabhaker, S. J. Castle & T. J. Henneberry, 2001. Inter-regional differences in baseline toxicity of Bemisia argentifolii (Homoptera: Aleyrodidae) to the two insect growth regulators, buprofezin and pyriproxyfen. Journal of Economic Entomology, 94 (6): 1538-1546.
44. TUIK, 2019. Turkish Statistical Institute: Crop production statistics. (Web page: http: //www.tuik.gov.tr) (Date accessed: January 2019) (in Turkish).
45. Wang, F., J. Liu, P. Chen, H. Li, J. Ma, Y. Liu & K. Wang, 2020. Bemisia tabaci (Hemiptera: Aleyrodidae) insecticide resistance in Shandong Province, China. Journal of Economic Entomology, 113 (2): 911-917.
46. Wang, R., J. D. Wang, W. N. Che & C. Luo, 2018. First report of field resistance to cyantraniliprole, a new anthranilic diamide insecticide, on Bemisia tabaci MED in China. Journal of Integrative Agriculture, 17 (1): 158-163.
47. Xie, W., Y. Liu, S. Wang, Q. Wu, H. Pan, X. Yang, L. Guo & Y. Zhang, 2014. Sensitivity of Bemisia tabaci (Hemiptera: Aleyrodidae) to several new insecticides in China: effects of insecticide type and whitefly species, strain and stage. Journal of Insect Science, 14 (1): 261-268.
48. Yükselbaba, U. & H. Göçmen, 2016. Determination of the resistance development potential to spiromesifen and the enzyme activities of the B and Q biotypes of cotton whitefly Bemisia tabaci (Genn.) (Hemiptera: Aleyrodidae). Fresenius Environmental Bulletin, 25 (7): 2461-2465.
49. Yükselbaba, U., C. İkten & H. Göçmen, 2012. “Determination of the biotypes of Bemisia tabaci (Genadius) (Hemiptera: Aleyrodidae) populations from Antalya Province of Turkey by sequence analysis of Mitochondrial Cytochrome Oxidase I (mtCOI) gene region, 29”. QBOL-EPPO Conference on DNA Barcoding and diagnostic methods for plant pests (21-25 June 2012, Haarlem, Netherlands), 71 pp.