Histochemical and ultrastructural analysis of macromolecules in trophocytes of the Oriental cockroach, Blatta orientalis (L., 1758) (Blattodea: Blattidae)

Yıl 2023, , 87 - 100, 25.04.2023

Tuğba Zülfikaroğlu , Gamze Turgay İzzetoğlu , Mehmet Salih Yıkılmaz , Savaş İzzetoğlu

https://doi.org/10.16970/entoted.1118639

Öz

The fat body is a major storage area for glycogen, lipid and protein. The trophocyte is the main cell of fat body and stores these macromolecules. The fat body consists of two parts; peripheral and perivisceral. Peripheral fat body is located below the integument while perivisceral fat body is around the digestive tract. The study was conducted in EGEMIKAL Analysis Laboratory and Histology Laboratory of Ege University between 2018 and 2020. The fat body contents of insects at all stages were examined comparatively in three selected sections through histochemical and ultrastructural studies. We identified macromolecules stored in the trophocytes. Both the granular form of proteins and asterisk structure of glycogen localized around the lipid droplets were observed clearly. It was found that accumulation of protein continued in the trophocytes, but glycogen accumulation decreased considerably in adults compared to all nymphal stages. We also found that larger lipid droplets were stored in the PF fat body, while glycogen and protein accumulation was much higher in the PV fat body. These results may contribute to understanding of the mechanisms underlying activities such as amino acid, nitrogen, lipid and carbohydrate metabolism and protein synthesis in insects.

Anahtar Kelimeler

Blatta orientalis, fat body, histochemistry, TEM, trophocyte

Destekleyen Kurum

Ege University Scientific Research Fund

Proje Numarası

FYL-2018-20133

Teşekkür

This work was supported by the Ege University Scientific Research Fund (FYL-2018-20133). Also, we are sincerely grateful to PhD Student Dirim Şendoğan for proofreading and Dr. Agâh Kozan for helping the statistical analyses.

Doğu hamam böceği Blatta orientalis (L., 1758) (Blattodea: Blattidae)’in trofositlerindeki makromoleküllerin histokimyasal ve ince yapı analizi

Öz

Böcek yağ dokusu glikojen, lipid ve protein için başlıca depolama alanıdır. Trofosit yağ dokusunun temel hücresidir ve bu makro molekülleri depolamaktadır. Yağ dokusu integümentin hemen altında yer alan periferal ve sindirim kanalının etrafında yer alan perivisseral yağ dokusu olmak üzere iki kısımdan oluşmaktadır. Bu çalışma 2018-2020 yılları arasında Ege Üniversitesi EGEMİKAL Analiz Laboratuvarı ve Histoloji Laboratuvarı’nda yürütülmüştür. Böceklerin tüm dönemlerine ait seçilen üç bölgesindeki yağ dokusu karşılaştırmalı olarak histokimyasal ve ince yapı çalışmalarıyla incelenmiştir. Trofositlerde depolanan makromoleküller belirlenmiştir. Hem granüler protein hem de lipid damlalarının etrafında yerleşim gösteren yıldız şeklindeki glikojen yapıları net bir şekilde gözlenmiştir. Tüm nimfal evrelerle erginler karşılaştırıldığında trofositlerde protein birikiminin devam ettiği, ancak glikojen birikiminin önemli ölçüde azaldığı belirlenmiştir. Ayrıca, periferal yağ dokusunda daha iri lipid damlaları depo edilirken, perivisseral yağ dokusunda ise daha fazla glikojen ve protein birikiminin olduğu tespit edilmiştir. Bu bulgular, böceklerde amino asit, nitrojen, lipid ve karbonhidrat metabolizmaları ve protein sentezi gibi faaliyetlerin altında yatan mekanizmaların anlaşılmasına katkı sağlayabilecektir.

Anahtar Kelimeler

Blatta orientalis, böcek yağ dokusu, histokimya, TEM, trofosit

Proje Numarası

FYL-2018-20133

Kaynakça

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