The UV protectant properties of tea extracts on entomopathogenic fungus spores and their lethal effect on Galleria mellonella (L., 1758) (Lepidoptera: Pyralidae)

Abstract

The present study aims to investigate the effectiveness of various tea extracts in providing ultraviolet (UV) protection for entomopathogenic fungi. UV radiation could have detrimental effects on viability of these fungi, which are important biocontrol agents against insect pests. This study was carried out in the Microbiology Laboratory of the Department of Biology Karadeniz Technical University in 2023. We evaluated the UV protective properties of various tea extracts in entomopathogenic fungi [Beauveria bassiana (Bals.) Vuil. (Hypocreales: Cordycipitaceae) and Metharizium flavoviride (Gams and Rozsypal 1956) (Hypocreales: Clavicipitaceae)] and tea extracts effectiveness against Galleria mellonella (L.,1758) (Lepidoptera: Pyralidae) larvae. Our findings demonstrate that certain tea extracts exhibit significant UV protection for entomopathogenic fungi, suggesting their potential application in improving the performance of biocontrol agents in outdoor environments. The highest UV-B protection was observed by adding black and green tea extracts to fungal spores, resulting in a radial growth measurement of 14.6 mm and 14.3 mm, respectively, at the end of 10 days of exposure for 120 minutes. These results contribute to the development of eco-friendly strategies for pest management in agriculture.

Keywords

• Beauveria bassiana
• Metharizium flavoviride
• UV protection
• tea extracts
• biological activity

Çay ekstraktlarının entomopatojen fungus sporları üzerindeki UV koruyucu özellikleri ve Galleria mellonella (L., 1758) (Lepidoptera: Pyralidae) üzerindeki öldürücü etkileri

Öz

Bu çalışma, entomopatojenik funguslar için çeşitli çay ekstraktlarının ultraviyole (UV) koruması etkinliğini araştırmayı amaçlamaktadır. UV radyasyonu, böcek zararlılarına karşı önemli biyokontrol etmenleri olan bu fungusların canlılığı üzerinde olumsuz etkilere sahip olabilir. Bu çalışma 2023 yılında Karadeniz Teknik Üniversitesi Biyoloji Bölümü Mikrobiyoloji Laboratuvarında yürütülmüştür. Çay ekstraktlarının entomopatojen funguslar [Beauveria bassiana (Bals.) Vuil. (Hypocreales: Cordycipitaceae) ve Metharizium flavoviride (Gams and Rozsypal 1956) (Hypocreales: Clavicipitaceae)] üzerindeki UV koruyucu özelliklerini ve Galleria mellonella (L.,1758) (Lepidoptera: Pyralidae) larvalarına karşı çay ekstraktlarının etkinliklerini değerlendirilmiştir. Bulgularımız, belirli çay özlerinin entomopatojen funguslar için önemli bir UV koruması sergilediğini göstermekte ve bu ekstraktların açık hava ortamlarında biyokontrol ajanlarının performansını artırmada potansiyel uygulamalarına işaret etmektedir. En yüksek UV-B koruması, fungus sporlarına siyah ve yeşil çay ekstraktları eklenerek elde edilmiş ve 10 günlük 120 dakikalık maruziyet sonunda sırasıyla 14.6 mm ve 14.3 mm’lik bir radyal büyüme ölçümü elde edilmiştir. Bu sonuçlar, tarımda çevre dostu zararlı yönetimi stratejilerinin geliştirilmesine katkıda bulunacaktır.

Anahtar Kelimeler

• Beauveria bassiana
• biyolojik aktivite
• Metharizium flavoviride
• çay ekstraktları
• UV koruma

Kaynakça

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